Action of Essential Fatty Acids on the Expression of Antioxidant Genes and Athletic Performance

NCT02177383 | Completed

• 1 other identifier: IB 994/08 PI
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

There are conflicting evidences of the effects of dietary supplementation with polyunsaturated fatty acids (PUFAs) on athletic performance. The investigators working hypothesis is based on the polyunsaturated fatty acids, given its pro-oxidative character and also by its action on transcription factors can modulate the antioxidant response and oxidative damage induced by physical activity.The overall objective of the study is to establish situations improving athletic performance and study the oxidative balance by manipulation of feeding patterns and consumption of nutritional supplements and investigate the involved mechanisms.

Conditions

Oxidative Stress; Exercise; Training Season; Antioxidant Capabilities; Athletic Performance

Outcome Measures

Primary Outcomes (1)

• DHA incorporation in erythrocyte cellular membranes

  Docosahexaenoic acid (DHA) is a conditionally essential fatty acid. Primary outcome: DHA incorporation in the erythrocyte cellular membranes by gas chromatography of soccer players during a training season

  Baseline, week 8

Secondary Outcomes (2)

• DHA on oxidative stress

  Baseline, week-8 (pre- and post-exercise)

• DHA on inflammation

  Baseline, week-8 (pre- and post-exercise)

Study Arms (2)

Docosahexaenoic acid

EXPERIMENTAL

1 liter/day of one experimental beverage (containing 0.2% olive oil + 0.6% DHA-S Martek) provides 1.14 g DHA/daily

Dietary Supplement: Docosahexaenoic acid diet supplementation

Olive oil

PLACEBO COMPARATOR

1 liter/day of placebo beverage (containing 0.8% olive oil)

Dietary Supplement: Placebo supplementation

Interventions

Docosahexaenoic acid diet supplementation DIETARY_SUPPLEMENT

Nine soccer players (19 and 22 years old) took 1 liter/day of one experimental beverage (containing 0.2% olive oil + 0.6% DHA-S Martek). Follow-up 8 weeks. At week 8 an acute exercise season was programed. One blood sample was taken in resting conditions at beginning of the nutritional intervention (week 0, baseline). Another two blood samples were taken at the end of the nutritional intervention, in resting (week 8, pre-exercise) and post-exercise conditions (week 8, post-exercise). Biochemical measures, oxidative damage markers on lipids, proteins and DNA, antioxidant capabilities (enzyme activities and quantity of enzyme protein and gene expression) and inflammatory markers were measured in serum, plasma, erythrocytes, neutrophils and peripheral blood mononuclear cells.

Docosahexaenoic acid

Placebo supplementation DIETARY_SUPPLEMENT

Six professionals soccer players (19 and 22 years old) took 1 liter/day of placebo beverage (containing 0.8% olive oil). Follow-up 8 weeks. At week 8 an acute exercise season was programed. One blood sample was taken in resting conditions at beginning of the nutritional intervention (week 0, baseline). Another two blood samples were taken at the end of the nutritional intervention, in resting (week 8, pre-exercise) and post-exercise conditions (week 8, post-exercise). Biochemical measures, oxidative damage markers on lipids, proteins and DNA, antioxidant capabilities (enzyme activities and quantity of enzyme protein and gene expression) and inflammatory markers were measured in serum, plasma, erythrocytes, neutrophils and peripheral blood mononuclear cells.

Olive oil

Eligibility Criteria

• Age: 16 Years - 35 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)

You may qualify if:

• Ages eligible for study: 16 to 35 years
• Genders eligible for study: Male
• Equilibrate diet
• Physical activity of 1-2 hours daily 5-7 days weekly.
• Body mass index (19-25 kg/m2)

You may not qualify if:

• Smokers

Sponsors & Collaborators

• University of the Balearic Islands: lead
• Instituto de Salud Carlos III: collaborator

Study Sites (1)

University of Balearic Islands

Palma de Mallorca, Balearic Islands, 07122, Spain

Location

Related Publications (3)

• Capo X, Martorell M, Sureda A, Llompart I, Tur JA, Pons A. Diet supplementation with DHA-enriched food in football players during training season enhances the mitochondrial antioxidant capabilities in blood mononuclear cells. Eur J Nutr. 2015 Feb;54(1):35-49. doi: 10.1007/s00394-014-0683-2. Epub 2014 Mar 19.

  PMID: 24643755 RESULT

• Martorell M, Capo X, Sureda A, Tur JA, Pons A. Effects of docosahexaenoic acid diet supplementation, training, and acute exercise on oxidative balance in neutrophils. Appl Physiol Nutr Metab. 2014 Apr;39(4):446-57. doi: 10.1139/apnm-2013-0331. Epub 2013 Nov 4.

  PMID: 24669986 RESULT

• Capo X, Martorell M, Sureda A, Tur JA, Pons A. Effects of dietary Docosahexaenoic, training and acute exercise on lipid mediators. J Int Soc Sports Nutr. 2016 Apr 5;13:16. doi: 10.1186/s12970-016-0126-y. eCollection 2016.

  PMID: 27051354 DERIVED

MeSH Terms

Conditions

Motor Activity

Condition Hierarchy (Ancestors)

Behavior

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor

Study Record Dates

• First Submitted: June 17, 2014
• First Posted: June 27, 2014
• Study Start: September 1, 2011
• Primary Completion: December 1, 2011
• Last Updated: June 27, 2014

Record last verified: 2014-06

Locations

ES (1)