Culturing TM Cells From Primary Open-angle Glaucoma Patient Biopsies

NCT02151318 | Completed

• 1 other identifier: 15309
• Study Type: observational
• Target: 12
• Locations: 1 country
• Sites: 2

Brief Summary

In this study, the investigators will collect tissue from patients undergoing trabeculectomy for primary open-angle glaucoma treatment. This tissue would normally be discarded. These trabecular meshwork (TM) cells can be cultured and used experimentally to better understand the mechanisms of glaucoma. These TM biopsies will be cultured on the same day as the trabeculectomy using established techniques.

Conditions

Glaucoma

Outcome Measures

Primary Outcomes (1)

• Efficacy of new glaucoma medications to reduce TM cellular pathologies associated with the disease glaucoma.

  TM cell biopsy samples from patients with glaucoma will be harvested and cultured in a laboratory setting to grow primary human TM cells. These cells will be used to test different medications, looking at the medications ability to reduce cellular pathologies commonly seen in TM tissue of patients with glaucoma. Pathologies include myocilin aggregation, and cell death. This will help researchers to determine the most effective compounds in treating glaucoma.

  6 weeks

Secondary Outcomes (1)

• Cultured TM cell toxicity caused by new glaucoma medications

  6 weeks

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients having glaucoma and undergoing a trabeculotomy

You may qualify if:

• Patients having glaucoma and undergoing a trabeculotomy
• Sign ICF (Informed Consent Form)

You may not qualify if:

• None

Sponsors & Collaborators

• University of South Florida: lead

Study Sites (2)

Tampa General Hosipital

Tampa, Florida, 33606, United States

Location

Byrd Alzheimer's Institute

Tampa, Florida, 33613, United States

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

The ophthalmologist will collect samples from the tissue that is removed during patients eye surgery that would otherwise be discarded or thrown away. Researchers will collect the sample and grow in the laboratory conditions. These cells will then be studied for further research on glaucoma.

MeSH Terms

Conditions

Glaucoma

Condition Hierarchy (Ancestors)

Ocular Hypertension; Eye Diseases

Study Officials

• Chad A Dickey, PhD

  University of South Florida department of Molecular Medicine and Byrd Alzheimer's Institute

  PRINCIPAL INVESTIGATOR

• Carla Bourne, MD

  University of South Florida and Tampa General Hospital

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: May 21, 2014
• First Posted: May 30, 2014
• Study Start: December 1, 2014
• Primary Completion: July 1, 2015
• Study Completion: November 1, 2016
• Last Updated: June 26, 2024

Record last verified: 2024-06

Locations

US (2)