NCT02151279

Brief Summary

Chitosan, the main component of the exoskeletons of crustaceans, mollusks and cephalopods, has been used as a fining agent in wines. However, its safety among patients allergic to shellfish has never been evaluated. Adult patients followed at the Allergy and Clinical Immunology Department who have been diagnosed with anaphylaxis to shrimp will be invited to participate in the study. Clinical data will be collected to ascertain for eligibility and written information will be provided. After signing informed consent, included subjects will perform skin prick-to-prick tests (PTP) with shrimp boiling water condensate and with fined and unfined wines. All will perform double blind oral challenge with the fined and unfined wines during 1visit day; the placebo (unfined wine) and active challenge (fined wine with chitosan) will be separated by 2 hours. Challenge protocol will be performed with successive increasing doses administered in 4 steps at 15-minute intervals for a total of 100 mL. During the challenge signs and symptoms will be monitored by a trained physician. Results will be presented as negative or positive (defined by presence of symptoms and signs of an allergic reaction). Categorical data will be compared by chi-square test. P\<0.05 will be considered statistically significant.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
15

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Mar 2014

Shorter than P25 for not_applicable

Geographic Reach
1 country

2 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

March 1, 2014

Completed
3 months until next milestone

First Submitted

Initial submission to the registry

May 20, 2014

Completed
10 days until next milestone

First Posted

Study publicly available on registry

May 30, 2014

Completed
1 month until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 1, 2014

Completed
2 months until next milestone

Study Completion

Last participant's last visit for all outcomes

September 1, 2014

Completed
Last Updated

May 30, 2014

Status Verified

May 1, 2014

Enrollment Period

4 months

First QC Date

May 20, 2014

Last Update Submit

May 29, 2014

Conditions

Keywords

Shrimp allergyChitosanfining agent

Outcome Measures

Primary Outcomes (1)

  • Number of participants with adverse events to a Double Blind Placebo Controlled Food Challenge with chitosan fined wine

    Participants will be followed until one to two hours after the end of the Food Challenge, an expected average of 6 to 8 hours

Study Arms (2)

control group

PLACEBO COMPARATOR

Chitosan as wine fining agent

Other: Control

Shrimp allergic patients

ACTIVE COMPARATOR

Chitosan as wine fining agent

Other: Chitosan as wine fining agent

Interventions

The possibility of allergic reactions attributed to traces of chitosan used as a fining agent in wine has not been ruled out, particularly in shrimp allergic patients to whom small traces of the potential allergen may be enough to trigger anaphylactic reactions.

Shrimp allergic patients
ControlOTHER
control group

Eligibility Criteria

Age18 Years - 65 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Aged above 18 and less than 65 years
  • Willing to comply with all study procedures and available for the duration of the study;
  • Diagnosed with shrimp allergy, based on clinical history, plus positive skin tests and/or positive IgE;
  • Previous anaphylactic reaction to shrimp;
  • Either sex and of any race
  • Provide signed and dated informed consent form

You may not qualify if:

  • Dermatological disease which precludes or alters the results of skin tests
  • History of wine/alcohol intolerance
  • Acquired or hereditary immunodeficiency
  • Neoplasia
  • Psychiatric disease
  • FEV1\<70%
  • Taking any systemic medication that might interfere with the study and that is not possible to withdraw, namely oral corticosteroids or immunomodulators in the last 4 weeks, or antihistamines in the last 10 days
  • Under beta-blockers or ACE inhibitors
  • Presence of any significant illness that could interfere with the study or alter its results or increase the risk of anaphylaxis, such as systemic mastocytosis
  • Pregnancy or lactation

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Universidade de Aveiro

Aveiro, Portugal

RECRUITING

Serviço de Imunoalergologia Hospital São João

Porto, 4200-239, Portugal

RECRUITING

Related Publications (1)

  • Amaral L, Silva D, Couto M, Nunes C, Rocha SM, Coimbra MA, Coimbra A, Moreira A. Safety of chitosan processed wine in shrimp allergic patients. Ann Allergy Asthma Immunol. 2016 May;116(5):462-3. doi: 10.1016/j.anai.2016.02.004. Epub 2016 Mar 19. No abstract available.

MeSH Terms

Conditions

Shellfish Hypersensitivity

Interventions

Chitosan

Condition Hierarchy (Ancestors)

Food HypersensitivityHypersensitivity, ImmediateHypersensitivityImmune System Diseases

Intervention Hierarchy (Ancestors)

ChitinBiopolymersPolymersMacromolecular SubstancesPolysaccharidesCarbohydrates

Study Officials

  • Luís P Amaral, MD

    * Intern

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 20, 2014

First Posted

May 30, 2014

Study Start

March 1, 2014

Primary Completion

July 1, 2014

Study Completion

September 1, 2014

Last Updated

May 30, 2014

Record last verified: 2014-05

Locations