Very Low Density Lipoprotein Turnover in Young and Elderly Individuals

NCT02113215 | Terminated

• 2 other identifiers: 11-3181R01AG033761-01A1
• Study Type: observational
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

Intrahepatic and intramyocellular lipid concentrations are elevated in elderly individuals relative to young people, despite no significant difference in fasting plasma TAG concentrations. Our general hypothesis is that the increase in intrahepatic fat with age results from reduced VLDL turnover, and therefore elderly individuals in this study will display lower VLDL turnover rates than young individuals. The aims of this study are a) to compare VLDL turnover in young and elderly individuals, and b) to compare a bolus method and a constant infusion method of assessing VLDL turnover.

Conditions

Aging

Keywords

aging; lipid metabolism; very low density lipoproteins

Outcome Measures

Primary Outcomes (1)

• Very Low Density Lipoprotein (VLDL) Turnover

  Turnover rates of VLDL-triglycerides and VLDL-ApoB (apolipoprotein B)-100, including synthesis and breakdown rates, measured during a 9-hour infusion trial.

  9-hours

Study Arms (4)

Young Males

Males, age 18-35 years. 9-hour Stable isotope infusion

Procedure: Stable Isotope Infusion

Young Females

Females, age 18-35 years. 9-hour Stable isotope infusion

Procedure: Stable Isotope Infusion

Older Males

Males, age 60-75. 9-hour Stable isotope infusion

Procedure: Stable Isotope Infusion

Older Females

Females, age 60-75. 9-hour Stable isotope infusion

Procedure: Stable Isotope Infusion

Interventions

Stable Isotope Infusion PROCEDURE

9-hour infusion of stable isotope tracers

Older Females; Older Males; Young Females; Young Males

Eligibility Criteria

• Age: 60 Years - 75 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Healthy individuals from the Galveston area.

You may qualify if:

• Age 18 to 35 years, or 60 to 75 years.
• Body mass index ≤ 27 kg•m2
• Ability to sign informed consent.
• Score of ≥26 for Mini-Mental State Exam

You may not qualify if:

• Diabetics (plasma glucose concentration \>200 mg/dl at 2 hr after oral intake of 75 g glucose).
• Fasting plasma triacylglycerol concentration above 500 mg/dl
• Kidney disease.
• Liver disease.
• Bleeding disorders.
• Anaemia (Hb \< 13 g/dl in males or \< 12 g/dl in females).
• Endocrine disease (with the exception of well-regulated hypothyroidism).
• Positive hepatitis or HIV screens.
• Alcohol (CAGE questionnaire; abnormal liver enzyme values) or drug abuse (amphetamines, cocaine, opioids, marijuana).
• Lipid altering agents.
• Score of \<26 for Mini-Mental State Exam

Sponsors & Collaborators

• The University of Texas Medical Branch, Galveston: lead
• National Institute on Aging (NIA): collaborator

Study Sites (1)

University of Texas Medical Branch, Dept of Surgery

Galveston, Texas, 77550, United States

Location

Biospecimen

Retention: SAMPLES WITH DNA

Blood samples and muscle biopsies will be collected.

Study Officials

• Elisabet Borsheim, Ph.D.

  University of Texas

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: April 10, 2014
• First Posted: April 14, 2014
• Study Start: December 1, 2011
• Primary Completion: July 1, 2013
• Study Completion: July 1, 2013
• Last Updated: May 12, 2016

Record last verified: 2016-05

Locations

US (1)