NCT02065310

Brief Summary

The purpose of this study is to see if the CEdG assay can produce internally consistent results and initiate its validation as a biomarker for diabetes in humans.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
26

participants targeted

Target at below P25 for not_applicable diabetes

Timeline
Completed

Started Oct 2014

Longer than P75 for not_applicable diabetes

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

January 31, 2014

Completed
19 days until next milestone

First Posted

Study publicly available on registry

February 19, 2014

Completed
7 months until next milestone

Study Start

First participant enrolled

October 1, 2014

Completed
4.4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 11, 2019

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

February 11, 2019

Completed
Last Updated

November 5, 2019

Status Verified

October 1, 2019

Enrollment Period

4.4 years

First QC Date

January 31, 2014

Last Update Submit

November 1, 2019

Conditions

Keywords

AGEDiabetes BiomarkersCEdG

Outcome Measures

Primary Outcomes (1)

  • Percentage of quality controls that fall within 15% of the nominal value for a analytical run of the CEdG assay.

    One time urine collection

Secondary Outcomes (1)

  • Correlation of Hemoglobin A1c and CEdG levels

    12 months

Study Arms (1)

Diabetes, Non-diabetes

NO INTERVENTION
Other: N2-(1-carboxyethyl)-2'deoxyguanosine (CEdG)

Interventions

Glucose and its adducts, such as HbA1c, decompose non-enzymatically to yield α-oxo-aldehydes are up to 20,000 x more reactive than the parent molecule. Amounts of an α-oxo-aldehyde, methylglyoxal (MG), are elevated up to 6-fold in patients with Type 1 dm. Quantitation of MG, but MG does form stable, irreversible adducts termed "advanced glycation endproducts" (AGEs) that can be measured. MG also reacts with DNA to yield primarily one stable DNA-AGE, CEdG, and suggests that measuring CEdG might allow for a direct method for assessing glycemic status and α-oxo-aldehyde burden. Investigators have shown that CEdG levels are significantly elevated in urine and tissue of Type 1 \& 2 dm animal models. DNA-AGE may correlate with the development of diabetes complications and identify patients at higher risk. Therefore, the investigators hypothesize that CEdG, a DNA-AGE, can be used as a marker of diabetes control and complications.

Diabetes, Non-diabetes

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • years of age or older
  • Registered patient of City of Hope
  • Documentation of a diagnosis of diabetes identified by the problem list in the patient's electronic health record

You may not qualify if:

  • Current pregnancy
  • An active diagnosis of cancer, as CEdG levels may potentially be affected by malignant processes

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

City of Hope Medical Center

Duarte, California, 91010, United States

Location

MeSH Terms

Conditions

Diabetes Mellitus

Condition Hierarchy (Ancestors)

Glucose Metabolism DisordersMetabolic DiseasesNutritional and Metabolic DiseasesEndocrine System Diseases

Study Officials

  • John Termini, PhD

    City of Hope Medical Center

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

January 31, 2014

First Posted

February 19, 2014

Study Start

October 1, 2014

Primary Completion

February 11, 2019

Study Completion

February 11, 2019

Last Updated

November 5, 2019

Record last verified: 2019-10

Locations