NCT02059538

Brief Summary

Supported by state-of-the-art systems medicine competences including integrative computational and functional genomics, the overarching goal of the trial is to investigate the impact of qualitative and quantitative changes in the gut microbiota on the pathogenesis of cardiometabolic diseases (CMDs) and their associated co-morbidities. A major objective will be to translate the clinical and fundamental based discoveries into new diagnosis and preventive actions paving the way to novel modes of treatment in the successive stages of CMD progression.

Trial Health

47
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
2,350

participants targeted

Target at P75+ for not_applicable

Timeline
Completed

Started Jun 2013

Typical duration for not_applicable

Geographic Reach
3 countries

3 active sites

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 1, 2013

Completed
5 months until next milestone

First Submitted

Initial submission to the registry

November 7, 2013

Completed
3 months until next milestone

First Posted

Study publicly available on registry

February 11, 2014

Completed
1.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2015

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2016

Completed
Last Updated

August 29, 2014

Status Verified

June 1, 2013

Enrollment Period

2 years

First QC Date

November 7, 2013

Last Update Submit

August 28, 2014

Conditions

Keywords

ObesityType 2 diabetesCoronary artery diseasesMicrobiotaOmics (ie transcriptomics, metabolomics, metagenomics, lipidomics)

Outcome Measures

Primary Outcomes (1)

  • Look for differences in gut microbiota signatures using metagenomic approach in the 8 different groups

    Look for differences in gut microbiota signatures using metagenomic approach in the 8 different groups (metabolic syndrome, type 2 diabetes, obesity, acute coronary event, chronic coronaropathy with or without cardiac insufficiency, cardiac insufficiency without coronaropathy and controls), in order to uncover gut derived signature associated with CMD stages

    baseline

Secondary Outcomes (1)

  • Establish differences in fecal metabolomic signatures using 1H nuclear magnetic resonance (NMR) spectroscopy and Ultra-high Performance Liquid Chromatography Mass Spectrometry ((UPLC-MS) on fecal samples) in the 8 different groups (cf above mentioned)

    baseline

Other Outcomes (6)

  • Establish differences in systemic and adipose tissue inflammatory patterns (using multiplex array and adipose tissue transcriptomic) in the 8 above mentioned groups

    Baseline

  • Establish host metabolomic differences in the 8 groups in order to obtain a CMD metabolomic derived signature (using 1H nuclear magnetic resonance) spectroscopy and Ultra-high Performance Liquid Chromatography Mass Spectrometry (in serum and urine)

    Baseline

  • Establish a statistical association between host metabolomic signatures (cf above 3 in serum and urine) and the gut metagenomic signatures in the 8 groups

    Baseline

  • +3 more other outcomes

Study Arms (8)

Group 1

OTHER

Metabolic syndrome

Other: Adipose tissue biopsies (omental and subcutaneous) liverRadiation: CT-scanOther: Stools samplingOther: DNA samplingOther: Blood samplingOther: Urine samplingRadiation: Dual energy X-ray absorptiometry-scan (DEXA-scan)

Group 2

OTHER

Severly obese patients

Other: Adipose tissue biopsies (omental and subcutaneous) liverRadiation: CT-scanOther: Stools samplingOther: DNA samplingOther: Blood samplingOther: Urine samplingRadiation: Dual energy X-ray absorptiometry-scan (DEXA-scan)

Group 3

OTHER

Type-2 diabetics patients

Other: Adipose tissue biopsies (omental and subcutaneous) liverRadiation: CT-scanOther: Stools samplingOther: DNA samplingOther: Blood samplingOther: Urine samplingRadiation: Dual energy X-ray absorptiometry-scan (DEXA-scan)

Group 4

OTHER

Patients with first recent (\< 2 weeks) acute coronary syndrome (ACS)

Other: Adipose tissue biopsies (omental and subcutaneous) liverRadiation: CT-scanOther: Stools samplingOther: DNA samplingOther: Blood samplingOther: Urine samplingRadiation: Dual energy X-ray absorptiometry-scan (DEXA-scan)

Group 5

OTHER

Patients with stable chronic coronary artery disease without heart failure

Other: Adipose tissue biopsies (omental and subcutaneous) liverRadiation: CT-scanOther: Stools samplingOther: DNA samplingOther: Blood samplingOther: Urine samplingRadiation: Dual energy X-ray absorptiometry-scan (DEXA-scan)

Group 6

OTHER

Patients with ischemic systolic heart failure (CHF)

Other: Adipose tissue biopsies (omental and subcutaneous) liverRadiation: CT-scanOther: Stools samplingOther: DNA samplingOther: Blood samplingOther: Urine samplingRadiation: Dual energy X-ray absorptiometry-scan (DEXA-scan)

Group 7

OTHER

Patients with non-ischemic chronic heart failure

Other: Adipose tissue biopsies (omental and subcutaneous) liverRadiation: CT-scanOther: Stools samplingOther: DNA samplingOther: Blood samplingOther: Urine samplingRadiation: Dual energy X-ray absorptiometry-scan (DEXA-scan)

Group 8

OTHER

Healthy volunteers

Other: Stools samplingOther: DNA samplingOther: Blood samplingOther: Urine samplingRadiation: Dual energy X-ray absorptiometry-scan (DEXA-scan)

Interventions

Group 1Group 2Group 3Group 4Group 5Group 6Group 7
CT-scanRADIATION
Group 1Group 2Group 3Group 4Group 5Group 6Group 7
Group 1Group 2Group 3Group 4Group 5Group 6Group 7Group 8
Group 1Group 2Group 3Group 4Group 5Group 6Group 7Group 8
Group 1Group 2Group 3Group 4Group 5Group 6Group 7Group 8
Group 1Group 2Group 3Group 4Group 5Group 6Group 7Group 8
Group 1Group 2Group 3Group 4Group 5Group 6Group 7Group 8

Eligibility Criteria

Age18 Years - 75 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Group 1 : metabolic syndrome As defined by the IDF
  • Central obesity: defined as waist circumference \> 94 cm for European men and \> 80 cm for European women (with knowledge on ethnicity for other groups)
  • plus 2 of the following criteria out of 4:
  • Elevated blood pressure with systolic ≥ 130 mmHg and/or diastolic ≥85 mmHg (or patients receiving anti-hypertensive drug treatment)
  • Triglycerides ≥1.50mg/dl (1.71mmol/l) (or patients receiving drug treatment for elevated triglycerides)
  • HDL-c\<40mg/dl (1.03 mmol/l) in males or HDL-c\<50mg/dl (1.29mmol/l) in females (or patients receiving drug treatment that reduces HDL-c)
  • Elevated fasting glucose: Glycemia ≥100mg/dl
  • Aged 18 to 75 years old
  • To avoid overlapping with the other groups (in particular group III): only patients with normal glucose tolerance (NGT), impaired glucose tolerance (IGT) and/or impaired fasting glucose (IFG) and an HbA1c \< 6.5 % will be included in this group.
  • Group II: severely Obese patients
  • Aged 18 to 75 years old
  • BMI ≥35kg/m²
  • Half of the effective will be standard obese patients (150 in Paris and 150 in Leipzig)
  • Half of the effective will be patients candidate to a bariatric surgery either Sleeve gastrectomy or Roux-en-Y bypass (with the following clinical conditions: according to European and national guidelines for obesity surgery):
  • BMI ≥40 kg/m² or
  • +21 more criteria

You may not qualify if:

  • \<18 or \>75 years old
  • Past history of abdominal cancer+/- radiation therapy on the abdomen
  • past history of intestinal resection except for appendicectomy
  • Participants with acute or chronic inflammatory or infectious diseases (including VHC, VHB and HIV)
  • Organ transplantation
  • Patient on Immunosuppressive therapy
  • Patients with severe kidney failure and or patients on dialysis therapy or eGFR \< 50 ml/min per 1.73 m2 body surface area)
  • Non affiliated to social security
  • Patients who do not understand the research procedures or those that are institutionalized, or those unable to give informed consent
  • Patients who decline participation
  • Patients with drug or alcohol addiction
  • Recent lost of weight \>10 kilos in the past 3 months
  • Recent antibiotic treatment (\<2months)
  • Patients on non-steroid anti-inflammatory treatment for the past 48 hours
  • Patients on laxative therapy that could modify the bowel transit in the past week
  • +13 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (3)

University of Copenhagen

Copenhagen, Denmark

RECRUITING

Hôpital Pitié-Salpétrière

Paris, 75013, France

RECRUITING

University of Leipzig

Leipzig, Germany

RECRUITING

Related Publications (1)

  • Belda E, Voland L, Tremaroli V, Falony G, Adriouch S, Assmann KE, Prifti E, Aron-Wisnewsky J, Debedat J, Le Roy T, Nielsen T, Amouyal C, Andre S, Andreelli F, Bluher M, Chakaroun R, Chilloux J, Coelho LP, Dao MC, Das P, Fellahi S, Forslund S, Galleron N, Hansen TH, Holmes B, Ji B, Krogh Pedersen H, Le P, Le Chatelier E, Lewinter C, Manneras-Holm L, Marquet F, Myridakis A, Pelloux V, Pons N, Quinquis B, Rouault C, Roume H, Salem JE, Sokolovska N, Sondertoft NB, Touch S, Vieira-Silva S; MetaCardis Consortium; Galan P, Holst J, Gotze JP, Kober L, Vestergaard H, Hansen T, Hercberg S, Oppert JM, Nielsen J, Letunic I, Dumas ME, Stumvoll M, Pedersen OB, Bork P, Ehrlich SD, Zucker JD, Backhed F, Raes J, Clement K. Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism. Gut. 2022 Dec;71(12):2463-2480. doi: 10.1136/gutjnl-2021-325753. Epub 2022 Jan 11.

MeSH Terms

Conditions

ObesityDiabetes Mellitus, Type 2Coronary Artery Disease

Interventions

Injections, SubcutaneousLiver ExtractsBlood Specimen CollectionAbsorptiometry, Photon

Condition Hierarchy (Ancestors)

OverweightOvernutritionNutrition DisordersNutritional and Metabolic DiseasesBody WeightSigns and SymptomsPathological Conditions, Signs and SymptomsDiabetes MellitusGlucose Metabolism DisordersMetabolic DiseasesEndocrine System DiseasesCoronary DiseaseMyocardial IschemiaHeart DiseasesCardiovascular DiseasesArteriosclerosisArterial Occlusive DiseasesVascular Diseases

Intervention Hierarchy (Ancestors)

InjectionsDrug Administration RoutesDrug TherapyTherapeuticsTissue ExtractsComplex MixturesSpecimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative TechniquesRadiographyDiagnostic ImagingDensitometryPhotometryChemistry Techniques, Analytical

Study Officials

  • Karine Clement, Professor

    Assistance Publique - Hôpitaux de Paris

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Karine Clement, Professor

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
SCREENING
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 7, 2013

First Posted

February 11, 2014

Study Start

June 1, 2013

Primary Completion

June 1, 2015

Study Completion

June 1, 2016

Last Updated

August 29, 2014

Record last verified: 2013-06

Locations