Effect of Darapladib on Cantharidin-Induced Inflammatory Blisters in Subjects With Type 2 Diabetes Mellitus (T2DM)

NCT02058641 | Completed Phase 1

• 1 other identifier: 200934
• Study Type: interventional
• Target: 9
• Locations: 1 country
• Sites: 1

Brief Summary

This will be an exploratory, open-label, single sequence, two part study (Part A and an optional Part B). The aim of this study will be to assess whether systemic inhibition of Lipoprotein associated phospholipase A2 (Lp-PLA2) in humans, effected by 11 days of once daily dosing to steady state with 160 milligrams (mg) of enteric coated (EC) darapladib, will specifically reduce the number of macrophages and/or result in a higher proportion of M2 macrophages in skin blisters induced by cantharidin (a chemical agent that causes blisters). In Part A of the study, a cohort of 8 subjects with type 2 diabetes mellitus will be recruited. In Part B of the study, a cohort of 8 additional healthy subjects with matching age (+/- 24 months) and gender to Part A may be recruited.

Conditions

Atherosclerosis

Keywords

cellular accumulation; macrophage phenotype; cantharidin blister; M1/M2; inflammatory mediators; Type 2 diabetes mellitus; darapladib; cytokines

Outcome Measures

Primary Outcomes (1)

• Macrophage cell count and surface expression of markers of M1 and M2 polarization in blister fluid

  To assess the effect of an 11 day course of once daily darapladib EC 160 mg on the number and phenotype (M1/M2 polarization) of macrophages isolated from blisters on subjects with type 2 diabetes mellitus (blisters induced by exposure to cantharidin for 48 hours). Biomarkers may include, but not be limited to: Cluster of differentiation (CD)11b, CD14, CD16, CD33, CD40, CD64, CD68, CD86, CD163, CD206, C-C chemokine receptor type 2 (CCR2), CX3 chemokine receptor 1 (CX3CR1)

  Up to 6 weeks

Secondary Outcomes (5)

• Number of monocytes and surface expression of markers of M1 or M2 polarization in peripheral blood

  Up to 6 weeks

• Enzymatic activity of Lp-PLA2 in blister fluid

  Up to 6 weeks

• Total cell count, lymphocyte count, neutrophil count and macrophage count in blister fluid

  Up to 6 weeks

• Concentrations of soluble mediators of macrophage polarization

  Up to 6 weeks

• Time to healing of cantharidin-induced blisters

  Up to 8 weeks

Study Arms (2)

Part A

EXPERIMENTAL

Part A will consist of 2 sessions. In Session 1, 3 blisters will be induced by a challenging agent (cantharidin solution 0.2 %, with 5 microliter administered topically) in T2DM subjects on Day 1. Blisters will be harvested 48 (+/-2) hour (hr) post induction. In Session 2, the same subjects will be administered darapladib EC tablet 160 mg orally, once daily for 11 days. On Day 10, 3 blisters will be induced by cantharidin. Blisters will be harvested 48 (+/-2) hr post induction.

Drug: Darapladib

Part B

EXPERIMENTAL

In Part B, healthy subjects will be enrolled and will follow the same dosing procedure as in Part A. The decision to initiate Part B will be made by the GSK study team based on an evaluation of data from Part A.

Drug: Darapladib

Interventions

Darapladib DRUG

EC tablet with a unit dose strength of 160 mg administered once daily orally for 11 days.

Part A; Part B

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• Male or female between 18 and 60 years of age inclusive, at the time of signing the informed consent.
• A female subject is eligible to participate if she is of: non-childbearing potential defined as pre-menopausal females with a documented tubal ligation or hysterectomy; or postmenopausal defined as 12 months of spontaneous amenorrhea \[in questionable cases a blood sample with simultaneous follicle stimulating hormone (FSH) \> 40 milli-international units (MIU)/milliliter (mL) and estradiol \< 40 picograms (pg)/mL (\<147 picomoles per liter \[pmol/L\]) is confirmatory\]. \[Females on hormone replacement therapy (HRT) and whose menopausal status is in doubt will be required to use one of the contraception methods listed in the protocol if they wish to continue their HRT during the study. Otherwise, they must discontinue HRT to allow confirmation of post-menopausal status prior to study enrollment. For most forms of HRT, at least 2-4 weeks should elapse between the cessation of therapy and the blood draw; this interval depends on the type and dosage of HRT. Following confirmation of their post-menopausal status, they can resume use of HRT during the study without use of a contraceptive method\]; Child-bearing potential and is abstinent (abstinence from penile-vaginal intercourse must be consistent with the preferred and usual lifestyle of the subject) or agrees to use one of the contraception methods listed in the protocol for an appropriate period of time (as determined by the product label or investigator) prior to the start of dosing to sufficiently minimize the risk of pregnancy at that point. Female subjects must agree to use contraception until 42 days after the last dose of study medication.
• Body mass index (BMI) within the range of 19.0-35.0 kilograms per meter square (kg/ m\^2) (inclusive).
• QTc interval Fridericia correction (QTcF) \<480 milliseconds (msec) in all subjects, including those with bundle branch block at screening electrocardiogram (ECG). Note that if the initial QTc value is prolonged, the ECG should be repeated two more times (with at least 5 minutes between ECG readings) and the average of the 3 QTc values used to determine eligibility.
• Additional Criteria for Diabetic Subjects
• A diagnosis of T2DM as determined by a responsible physician based on a medical evaluation including medical history, physical examination, and laboratory tests, with onset at least 6 months prior to screening and on stable treatment for 3 months prior to screening.
• Subjects have no recent changes or anticipation of future changes in anti-hyperglycaemic therapies during the 3-month period before and during the study respectively.
• Subjects will have good peripheral pulses and no peripheral sensory loss as determined by physical examination.
• Glycosylated hemoglobin (HbA1c) levels \<= 8.0% at screening. Additional Criteria for Healthy Subjects (if recruited in Part B)
• Healthy as determined by a responsible and experienced physician, based on a medical evaluation including medical history, physical examination, vital signs, complete blood count and clinical chemistry. A subject with a clinical abnormality or laboratory parameters outside the reference range for the population being studied may be included only if the Investigator and the GSK Medical Monitor agree that the finding is unlikely to introduce additional risk factors and will not interfere with the study procedures.

You may not qualify if:

• A positive pre-study Hepatitis B surface antigen or positive Hepatitis C antibody result within 3 months of screening
• Current or chronic history of liver disease or known hepatic or biliary abnormalities (with the exception of Gilbert's syndrome or asymptomatic gallstones). Abnormal liver function tests at screening. For healthy subjects: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase and bilirubin \>=1.5xupper limit of normal (ULN) (isolated bilirubin \>1.5xULN is acceptable if bilirubin is fractionated and direct bilirubin \<35%) at screening.
• A positive pre-study drug/alcohol screen.
• A positive test for HIV antibody.
• History of regular alcohol consumption within 6 months of the study defined as: For United Kingdom (UK) sites: an average weekly intake of \>21 units for males or \>14 units for females. One unit is equivalent to 8 grams (g) of alcohol: a half-pint (approximately 240 mL) of beer, 1 glass (125 mL) of wine or 1 (25 mL) measure of spirits.
• The subject has participated in a clinical trial and has received an investigational product within the following time period prior to the first dosing day in the current study: 90 days, 5 half-lives or twice the duration of the biological effect of the investigational product (whichever is longer).
• Exposure to more than four new chemical entities within 12 months prior to the first dosing day.
• Unable to refrain from the use of prescription drugs taken on an intermittent (as needed) basis or non-prescription drugs; these include vitamins, herbal and dietary supplements (including St John's Wort) within 7 days (or 14 days if the drug is a potential enzyme inducer) or 5 half-lives (whichever is longer) prior to Day 1 of session 1 and continuing until the final follow up visit (with the exception of paracetamol).
• History of sensitivity to any of the study medications, or components thereof or a history of drug or other allergy that, in the opinion of the Investigator or GSK Medical Monitor, contraindicates their participation.
• History of anaphylaxis, and /or anaphylactoid (resembling anaphylaxis) reactions, or severe allergic responses.
• Where participation in the study would result in donation of blood or blood products in excess of 500 mL within a 56 day period.
• Currently in a study of an investigational device.
• Pregnant females (as determined by positive serum beta human chorionic gonadotropin test at screening and on Day1 of Session 1, and pre-dose on day 1 of session 2) or lactating females.
• Unwillingness or inability to follow the procedures outlined in the protocol.
• Subject is mentally or legally incapacitated.

Sponsors & Collaborators

• GlaxoSmithKline: lead

Study Sites (1)

GSK Investigational Site

Cambridge, CB2 0GG, United Kingdom

Location

Related Links

• Results for study 200934 can be found on the GSK Clinical Study Register.

MeSH Terms

Conditions

Atherosclerosis; Diabetes Mellitus, Type 2

Interventions

darapladib

Condition Hierarchy (Ancestors)

Arteriosclerosis; Arterial Occlusive Diseases; Vascular Diseases; Cardiovascular Diseases; Diabetes Mellitus; Glucose Metabolism Disorders; Metabolic Diseases; Nutritional and Metabolic Diseases; Endocrine System Diseases

Study Officials

• GSK Clinical Trials

  GlaxoSmithKline

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: phase 1
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: INDUSTRY
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 6, 2014
• First Posted: February 10, 2014
• Study Start: February 26, 2014
• Primary Completion: August 18, 2014
• Study Completion: August 18, 2014
• Last Updated: May 15, 2017

Record last verified: 2017-05

Locations

GB (1)