NCT02043964

Brief Summary

This is a multicentric and prospective study for the validation of a diagnostic method in multiple sclerosis. Our hypothesis is that there is a characteristic profile of tears that can be recognized in order to help in the diagnosis of this pathology and that could possibly replace the lumbar punction which is an invasive method. Semi-automatic and automatic techniques of isoelectric focusing will be developed for analyzing data from tears. These results will help in the identification of markers of this disease.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
165

participants targeted

Target at P75+ for not_applicable

Timeline
Completed

Started Oct 2012

Longer than P75 for not_applicable

Geographic Reach
1 country

15 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

October 1, 2012

Completed
1.1 years until next milestone

First Submitted

Initial submission to the registry

November 22, 2013

Completed
2 months until next milestone

First Posted

Study publicly available on registry

January 23, 2014

Completed
4.4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2018

Completed
6 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2018

Completed
Last Updated

March 26, 2024

Status Verified

March 1, 2024

Enrollment Period

5.7 years

First QC Date

November 22, 2013

Last Update Submit

March 25, 2024

Conditions

Keywords

Multiple SclerosisClinically Isolated SyndromeTearsIsoelectric focusingOligoclonal profile

Outcome Measures

Primary Outcomes (1)

  • Test of the concordance coefficient between the analysis of tears and the analysis of cerebrospinal fluid

    Test of the concordance coefficient between the analysis of tears and the analysis of cerebrospinal fluid

    two years after

Secondary Outcomes (5)

  • Diagnostic performance of isoelectric focusing of tears

    inclusion, two years after

  • Concordance between visual and automatic reading of electrophoretic profiles

    two years

  • Calculation of sensitivity/specificity

    two years

  • Calculation of positive/negative predictive value

    two years

  • Calculation of efficiency odds ratio

    two years

Study Arms (1)

tears and cerebro-spinal fluid sampling

EXPERIMENTAL
Procedure: tears and cerebrospinal fluid sampling

Interventions

All patients recruited in this study will undergo a cerebrospinal fluid sampling which is an invasive procedure included in the actual routine practice. In addition a sampling from eyes will be obtain using the Schirmer test to get tears from the lacrimal gland.

tears and cerebro-spinal fluid sampling

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • years old or more
  • Clinically isolated syndrome of less than 3 months of onset
  • Informed consent to participate

You may not qualify if:

  • Progressive primary multiple sclerosis
  • Relapsing remitting multiple sclerosis
  • Persons wearing contact lenses
  • Ocular Infection
  • Corticoid treatment at least 30 days before sampling
  • Immunosuppressive or immunomodulatory treatment at least 3 months before sampling
  • Pregnancy or breast feeding
  • No coverage by the Social Insurance

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (15)

Centre Hospitalier Universitaire de Bordeaux

Bordeaux, 33300, France

Location

Centre Hospitalier Universitaire Caen

Caen, 14000, France

Location

Centre Hospitalier Universitaire de Clermont-Ferrand

Clermont-Ferrand, 63000, France

Location

Centre Hospitalier Universitaire de Dijon

Dijon, 21000, France

Location

Centre Hospitalier Régional Universitaire de Lille

Lille, 59000, France

Location

Groupe Hospitalier de l'Institut Catholique de Lille

Lomme, 59 462, France

Location

Centre Hospitalier Universitaire de Lyon

Lyon, 69000, France

Location

Hopital de la Timone

Marseille, 13000, France

Location

Universitaire de Nancy

Nancy, 54100, France

Location

Centre Hospitalier Universitaire de Nantes

Nantes, 44200, France

Location

Centre Hospitalier Universitaire de Nice

Nice, 6200, France

Location

Centre Hospitalier Intercommunal Poissy-St-Germain

Poissy, 78300, France

Location

Centre Hospitalier Univetrsitaire de Rouen

Rouen, 76100, France

Location

Centre Hospitalier Régional Universitaire de Strasbourg

Strasbourg, 67100, France

Location

Centre Hospitalier de Valenciennes

Valenciennes, 59300, France

Location

MeSH Terms

Conditions

Multiple SclerosisLacerations

Condition Hierarchy (Ancestors)

Demyelinating Autoimmune Diseases, CNSAutoimmune Diseases of the Nervous SystemNervous System DiseasesDemyelinating DiseasesAutoimmune DiseasesImmune System DiseasesWounds and Injuries

Study Officials

  • Gérard Forzy, MD

    Groupement des Hôpitaux de l'Institut Catholique de Lille

    STUDY DIRECTOR
  • Patrick Hautecoeur, MD

    Groupement des Hôpitaux de l'Institut Catholique de Lille

    PRINCIPAL INVESTIGATOR
  • Patrick Vermersch, MD, PhD

    Centre Hospitalier Régional, Universitaire de Lille

    PRINCIPAL INVESTIGATOR
  • Gilles Defer, MD

    Centre Hospitalier Universitaire Caen

    PRINCIPAL INVESTIGATOR
  • Christine Lebrun-Fresnay, MD, PhD

    Centre Hospitalier Universitaire de Nice

    PRINCIPAL INVESTIGATOR
  • Jérôme de Seze, MD, PhD

    Centre Hospitalier Régional Universitaire de Strasbourg

    PRINCIPAL INVESTIGATOR
  • Thibault Moreau, MD

    Centre Hospitalier Universitaire Dijon

    PRINCIPAL INVESTIGATOR
  • Pierre Clavelou, MD

    University Hospital, Clermont-Ferrand

    PRINCIPAL INVESTIGATOR
  • Olivier Heinzlef, MD

    Centre Hospitalier Intercommunal Poissy-St-Germain

    PRINCIPAL INVESTIGATOR
  • Christian Confavreux, MD

    Centre Hospitalier Universitaire de Lyon

    PRINCIPAL INVESTIGATOR
  • Marc Debouverie, MD, PhD

    Central Hospital, Nancy, France

    PRINCIPAL INVESTIGATOR
  • Jean Pelletier, MD, PhD

    Hopital de la Timone, Marseille

    PRINCIPAL INVESTIGATOR
  • Bruno Brochet, MD, PhD

    University Hospital, Bordeaux

    PRINCIPAL INVESTIGATOR
  • Sandrine Wiertlewski, MD

    Nantes University Hospital

    PRINCIPAL INVESTIGATOR
  • Albert Verier, MD

    Centre Hospitalier de Valenciennes

    PRINCIPAL INVESTIGATOR
  • Bertrand Bourre, MD

    University Hospital, Rouen

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NA
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

November 22, 2013

First Posted

January 23, 2014

Study Start

October 1, 2012

Primary Completion

June 1, 2018

Study Completion

December 1, 2018

Last Updated

March 26, 2024

Record last verified: 2024-03

Locations