NCT01991626

Brief Summary

Previous studies on iron absorption have focused on the effects on the addition of iron absorption enhancers like ascorbic acid and EDTA to a meal and more recently the enzymatic phytic acid removal through phytase. The investigators want here to investigate the effects of fat on iron absorption with and without the addition of exogenous phytase as a potential iron absorption enhancer in lipid nutrient supplements (LNS) products compared to micronutrient powder(MNP) products. Furthermore the investigators will evaluate the effects of Lipids- on iron absorption to induce the ileal brake by ingestion of a fat emulsion prior to a meal. Increasing the caloric density of a meal by increasing its fat content might enhance iron absorption by delaying gastric emptying, gastric acid secretion and increasing Fe stomach residence time.

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
47

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Sep 2013

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

September 1, 2013

Completed
2 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 1, 2013

Completed
17 days until next milestone

First Submitted

Initial submission to the registry

November 18, 2013

Completed
7 days until next milestone

First Posted

Study publicly available on registry

November 25, 2013

Completed
8 months until next milestone

Study Completion

Last participant's last visit for all outcomes

August 1, 2014

Completed
Last Updated

September 23, 2015

Status Verified

September 1, 2015

Enrollment Period

2 months

First QC Date

November 18, 2013

Last Update Submit

September 22, 2015

Conditions

Outcome Measures

Primary Outcomes (1)

  • Iron bio-availability from Oral Iron Supplements

    Iron bioavailability will be assessed with stable isotopic labels. The shift in the isotopic ratio in human whole blood 14 days after administration will be measured with mass spectrometry.

    14 days

Study Arms (10)

LNS

OTHER

Maize meal mixed with Lipid Nutrient Supplement (LNS) containing micronutrient powder

Dietary Supplement: micronutrient powder

FePP-Emulsion mixed

OTHER

Fat emulsion mixed to the meal containing FePP

Dietary Supplement: Iron pyrophosphate (FePP)

FeSO4-Mixed

OTHER

meals containing FeSO4 mixed with a fat emulsion

Dietary Supplement: Iron sulphate

FePP-Emulsion before

OTHER

Fat emulsion taken before a meal containing FePP

Dietary Supplement: Iron pyrophosphate (FePP)

FeSO4- Emulsion before

OTHER

Fat emulsion taken before a maize meal containing FeSO4

Dietary Supplement: Iron sulphate

LNS-Phytase

OTHER

Maize meal mixed with LNS containing micronutrient powder and phytase

Dietary Supplement: micronutrient powder (containing FeSO4), phytase

phytase

OTHER

Maize meal containing micronutrient powder and phytase

Dietary Supplement: micronutrient powder (containing FeSO4), phytase

MNP-control

OTHER

maize meal containing micronutrient powder (MNP)

Dietary Supplement: micronutrient powder

FePP control

OTHER

Maize meal containing FePP

Dietary Supplement: Iron pyrophosphate (FePP)

FeSO4 control

OTHER

Maize meal containing FeSO4

Dietary Supplement: Iron sulphate

Interventions

micronutrient powderDIETARY_SUPPLEMENT
LNSMNP-control
LNS-Phytasephytase
Iron pyrophosphate (FePP)DIETARY_SUPPLEMENT
FePP controlFePP-Emulsion beforeFePP-Emulsion mixed
Iron sulphateDIETARY_SUPPLEMENT
FeSO4 controlFeSO4- Emulsion beforeFeSO4-Mixed

Eligibility Criteria

Age18 Years - 45 Years
Sexfemale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Reproductive age females 18-45 years
  • Normal body mass index (17.5-25 kg/m2)
  • No intake of mineral/vitamin supplements 2 weeks before and during the study
  • No metabolic or gastrointestinal disorders
  • No food allergies or other chronic disorders or eating disorders assessed by self-report
  • Having received full oral and written information about the aims and procedures of the study
  • Willing to comply with the study procedure
  • Having provided oral and written informed consent

You may not qualify if:

  • Regular intake of medication (except oral contraceptives)
  • Blood donation or significant blood loss (accident, surgery) over the past 4 months
  • Currently participating in another clinical trial or having participated in another clinical trial during the last 30 days prior to the beginning of this study
  • Former participation in a study involving administration of iron stable isotopes
  • Subject who cannot be expected to comply with study protocol
  • Pregnancy or lactation

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Related Publications (1)

  • Monnard A, Moretti D, Zeder C, Steingotter A, Zimmermann MB. The effect of lipids, a lipid-rich ready-to-use therapeutic food, or a phytase on iron absorption from maize-based meals fortified with micronutrient powders. Am J Clin Nutr. 2017 Jun;105(6):1521-1527. doi: 10.3945/ajcn.116.142976. Epub 2017 May 3.

MeSH Terms

Conditions

Iron Deficiencies

Interventions

6-PhytaseIron-Dextran Complex

Condition Hierarchy (Ancestors)

Iron Metabolism DisordersMetabolic DiseasesNutritional and Metabolic Diseases

Intervention Hierarchy (Ancestors)

Phosphoric Monoester HydrolasesEsterasesHydrolasesEnzymesEnzymes and CoenzymesCoordination ComplexesOrganic ChemicalsDextransGlucansPolysaccharidesCarbohydrates

Study Officials

  • Michael B Zimmermann, Prof

    ETH Zürich

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
PARTICIPANT
Purpose
BASIC SCIENCE
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Prof

Study Record Dates

First Submitted

November 18, 2013

First Posted

November 25, 2013

Study Start

September 1, 2013

Primary Completion

November 1, 2013

Study Completion

August 1, 2014

Last Updated

September 23, 2015

Record last verified: 2015-09