NCT01930032

Brief Summary

The purpose of this study is to learn more about infection by Clostridium difficile (also known as C. difficile). C. difficile is a common bacterium (a germ that may cause disease) that can live in the human gut. Some people have it without having any symptoms. In other people it can cause illness ranging from mild diarrhea to severe colitis (infection of the colon). C. difficile makes toxins that damage the cells that line the colon. The study doctors want to find out how these toxins cause damage to the cells in the colon.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
24

participants targeted

Target at below P25 for all trials

Timeline
Completed

Started Aug 2013

Shorter than P25 for all trials

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

August 1, 2013

Completed
19 days until next milestone

First Submitted

Initial submission to the registry

August 20, 2013

Completed
8 days until next milestone

First Posted

Study publicly available on registry

August 28, 2013

Completed
4 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 1, 2014

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 1, 2014

Completed
Last Updated

March 8, 2017

Status Verified

March 1, 2017

Enrollment Period

5 months

First QC Date

August 20, 2013

Last Update Submit

March 6, 2017

Conditions

Keywords

Clostridium difficile infection

Outcome Measures

Primary Outcomes (1)

  • Binding of Toxin A (and B) to TLR9 on the human colorectal epithelial cell surface

    As assessed by confocal fluorescence microscopy

    24 hours

Secondary Outcomes (5)

  • effects of a TLR9 antagonist (ODN-TTAGGG) on toxin binding

    0 hours

  • Binding of Toxin A (and B) to TLR9 on the human colorectal epithelial cell surface

    0 hours

  • Binding of Toxin A (and B) to TLR9 on the human colorectal epithelial cell surface

    6 hours

  • Effects of a TLR9 antagonist (ODN-TTAGGG) on toxin binding

    6 hours

  • Effects of a TLR9 antagonist (ODN-TTAGGG) on toxin binding

    24 hours

Study Arms (1)

All subjects

Eligibility Criteria

Age18 Years - 75 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Subjects will be selected from patients undergoing a routine screening colonoscopy at Beth Israel Deaconess Medical Center

You may qualify if:

  • Age greater than 18 yrs and less than 75 years
  • Undergoing a clinically indicated colonoscopy

You may not qualify if:

  • Diarrhea (an average of more than 3 bowel movements per day at baseline)
  • Constipation (an average of fewer than 2 bowel movements per week at baseline).
  • Use of systemic steroid or systemic immunosuppressive medication
  • Severe renal impairment
  • Relative contraindication to colon biopsy including a bleeding diathesis or anti-coagulant use. Note: nonsteroidal antiinflammatory drug or asprin use will not constitute a contra-indication.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Beth Israel Deaconess Medical Center

Boston, Massachusetts, 02215, United States

Location

Biospecimen

Retention: SAMPLES WITH DNA

Colonic biopsy samples

MeSH Terms

Conditions

Clostridium Infections

Condition Hierarchy (Ancestors)

Gram-Positive Bacterial InfectionsBacterial InfectionsBacterial Infections and MycosesInfections

Study Design

Study Type
observational
Observational Model
OTHER
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Professor of Medicine

Study Record Dates

First Submitted

August 20, 2013

First Posted

August 28, 2013

Study Start

August 1, 2013

Primary Completion

January 1, 2014

Study Completion

January 1, 2014

Last Updated

March 8, 2017

Record last verified: 2017-03

Locations