NCT01925560

Brief Summary

The objective of this study is to assess the tolerance and utilization of agave inulin. This will be accomplished through analyses of breath and fecal samples to quantify fermentation end-products- hydrogen, carbon dioxide, and methane will be measured in the breath, and short-chain fatty acids, ammonia, phenol, and indole will be measured in feces. Microbial populations will also be measured in fecal samples.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
30

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Jan 2013

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 1, 2013

Completed
4 months until next milestone

First Submitted

Initial submission to the registry

April 17, 2013

Completed
14 days until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 1, 2013

Completed
4 months until next milestone

First Posted

Study publicly available on registry

August 19, 2013

Completed
9 months until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2014

Completed
Last Updated

May 14, 2014

Status Verified

May 1, 2014

Enrollment Period

4 months

First QC Date

April 17, 2013

Last Update Submit

May 13, 2014

Conditions

Outcome Measures

Primary Outcomes (2)

  • Breath gas

    On the 21st day of each treatment period, participants will come into the laboratory after an overnight (12 hour) fast and begin breath gas testing. The baseline breath gas sample will occur upon arrive. Breath gas collection will utilize the Quintron EasySampler and tubes. Participants will blow into the EasySampler for 1-2 seconds while inserting a tube into a needle holder that allows the collection of the breath gas. After the baseline sample, the participants will consume their respective treatments. Participants then collect subsequent breath gas samples outside of the laboratory every hour for the next 8 hours. All breath gas samples will then be analyzed for CO2, methane, and H2 gases.

    21st day of each treatment period

  • Gastrointestinal tolerance

    Participants will complete daily and weekly questionnaires to assess tolerance of up to 7.5 g/d agave inulin. Adults completed a weekly survey while meeting with study personnel. They were asked (Yes or No) if they experienced any of the following gastrointestinal symptoms during the past 7 days: nausea, bloating, gastrointestinal rumblings, gas/flatulence, abdominal pain, diarrhea (watery stools). If yes, they rated the intensity of the symptom as no more than usual (0), somewhat more than usual (1), or much more than usual (2). Participants are provided a journal to complete on their own each day, a questionnaire about their stool characteristics. Participants were asked to score the ease of stool passage using the following scale: 1=very easy, 2=easy, 3=neither easy nor difficult, 4=difficult, 5=very difficult.

    Daily and weekly

Secondary Outcomes (6)

  • Fecal fermentation end products

    Days 16-20 of each treatment period

  • Fecal bacteria

    Days 16-20 of each treatment period

  • Gastrointestinal Tolerance

    Daily during each 21 day treatment period

  • Weekly Gastrointestinal Tolerance Questions

    Weekly during each treatment period

  • Daily Stool Characteristics

    Daily during each 21 day treatment period

  • +1 more secondary outcomes

Study Arms (3)

Group A- agave inulin or placebo

EXPERIMENTAL

Participant in this group will receive Agave Inulin dose of 5 or 7.5 grams per day or placebo.

Other: Agave InulinOther: Placebo

Group B- agave inulin or placebo

EXPERIMENTAL

Participants in this group will receive Agave Inulin dose of 5 or 7.5 grams per day or placebo

Other: Agave InulinOther: Placebo

Group C-agave inulin or placebo

EXPERIMENTAL

Participants in this group will receive Agave Inulin dose of 5 or 7.5 grams per day or placebo

Other: Agave InulinOther: Placebo

Interventions

3x3 Latin square with 3 periods

Group A- agave inulin or placeboGroup B- agave inulin or placeboGroup C-agave inulin or placebo
PlaceboOTHER

control- 0 (zero) grams/day inulin

Group A- agave inulin or placeboGroup B- agave inulin or placeboGroup C-agave inulin or placebo

Eligibility Criteria

Age20 Years - 40 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • have body mass index (BMI) between 18.5 and 29.5 kg/m2
  • free of metabolic and gastrointestinal diseases

You may not qualify if:

  • BMI less than 18.5 or greater than 29.5 kg/m2
  • presence of metabolic and gastrointestinal diseases
  • pregnant or lactating
  • taking medications that impact bowel function

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of Illinois

Urbana, Illinois, 61801, United States

Location

Related Publications (2)

  • Kaczmarek JL, Musaad SM, Holscher HD. Time of day and eating behaviors are associated with the composition and function of the human gastrointestinal microbiota. Am J Clin Nutr. 2017 Nov;106(5):1220-1231. doi: 10.3945/ajcn.117.156380. Epub 2017 Sep 27.

  • Holscher HD, Bauer LL, Gourineni V, Pelkman CL, Fahey GC Jr, Swanson KS. Agave Inulin Supplementation Affects the Fecal Microbiota of Healthy Adults Participating in a Randomized, Double-Blind, Placebo-Controlled, Crossover Trial. J Nutr. 2015 Sep;145(9):2025-32. doi: 10.3945/jn.115.217331. Epub 2015 Jul 22.

Study Officials

  • Kelly Swanson, PhD

    UIUC

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
BASIC SCIENCE
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

April 17, 2013

First Posted

August 19, 2013

Study Start

January 1, 2013

Primary Completion

May 1, 2013

Study Completion

May 1, 2014

Last Updated

May 14, 2014

Record last verified: 2014-05

Locations