NCT01909063

Brief Summary

The goal of this investigator-initiated study is to determine whether the fortification of orange juice with vitamin D, vitamin A, and vitamin E will enhance the vitamin D, vitamin A, and vitamin E status children ages 6-10 that are seen at the Division of Pediatrics at Boston University Medical Center. Circulating concentrations of 25-hydroxyvitamin D \[25(OH)D\], vitamin A, and vitamin E before, will be measured at mid-intervention (week 6), and after a period of twelve weeks. This study plans to recruit 180 male and female subjects between the ages of 6 and 10. An informed consent will be explained and discussed with the subjects and their parents/guardians willing to participate in the study. The study will be twelve weeks. Blood will be drawn during the initial visit, mid-intervention (week 6), and week 12. Dietary intake will be assessed at baseline and at the conclusion of the 12-week intervention using a 3-day food record. The subjects will be randomized in a double-blinded manner via an electronically shuffled listed. Subjects will be randomized to receive one of three beverages: (1) calcium plus vitamin D fortified orange juice (intervention A), (2) calcium plus vitamins D, A, and E fortified orange juice (intervention B) or (3) calcium-only fortified orange juice (controls). Subjects in all groups will drink two 8-oz. glasses of juice at least six hours apart (morning and afternoon) per day for a period of 12 weeks. Subjects randomized to intervention A will receive 200 IU vitamin D and 700 mg of calcium per day in 2 glasses of juice, intervention B will receive 200 IU vitamin D, 12 IU vitamin E, 2000 IU vitamin A as beta carotene, and 700 mg of calcium per day in 2 glasses of juice, while controls will receive 700 mg of calcium per day in 2 glasses of juice. A blood sample will be obtained before the subjects begin drinking the orange juice and at week 12 to determine levels of 25(OH)D which is a measure of vitamin D status. Blood will also be used for determining osteocalcin, parathyroid hormone (PTH), alkaline phosphatase, phosphorus, calcium, C-telopeptide (CTX), albumin, vitamin A, and vitamin E. A blood sample will also be obtained at week 6 for 25(OH)D and PTH.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
180

participants targeted

Target at P75+ for not_applicable

Timeline
Completed

Started Jan 2005

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 1, 2005

Completed
3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 1, 2008

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 1, 2008

Completed
4.5 years until next milestone

First Submitted

Initial submission to the registry

June 25, 2012

Completed
1.1 years until next milestone

First Posted

Study publicly available on registry

July 26, 2013

Completed
Last Updated

February 28, 2017

Status Verified

February 1, 2017

Enrollment Period

3 years

First QC Date

June 25, 2012

Last Update Submit

February 27, 2017

Conditions

Outcome Measures

Primary Outcomes (1)

  • Improve Vitamin A, Vitamin E, and Vitamin D Status in Children

    The primary outcome of this study is to determine if fortification of orange juice with vitamin D, vitamin A, and vitamin E will enhance the vitamin D, vitamin A, and vitamin E status children ages 6-10 that are seen at the Division of Pediatrics at Boston University Medical Center.

    12 weeks

Study Arms (3)

Arm A

EXPERIMENTAL

200 IU vitamin D and 700 mg of calcium per day in 2 glasses of juice per day for 2 weeks

Dietary Supplement: Vitamin D

Arm B

EXPERIMENTAL

200 IU vitamin D, 12 IU vitamin E, 2000 IU vitamin A as beta carotene, and 700 mg of calcium per day in 2 glasses of juice Vitamin D, Vitamin E, and Vitamin A

Dietary Supplement: Vitamin D, Vitamin E, and Vitamin A

Arm C

PLACEBO COMPARATOR

Control, 700 mg of calcium per day in 2 glasses of juice Control, 700 mg Calcium

Dietary Supplement: Control, 700 mg Calcium

Interventions

Vitamin DDIETARY_SUPPLEMENT

Fortification of orange juice with 200 IU vitamin D to be drunk twice per day for 12 weeks

Arm A

Fortification of orange juice with Vitamin D, Vitamin E, and Vitamin A to be drunk twice per day for 12 weeks

Arm B
Control, 700 mg CalciumDIETARY_SUPPLEMENT

Orange juice with 700 mg calcium to be drunk twice per day for 12 weeks

Arm C

Eligibility Criteria

Age6 Years - 10 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17)

You may qualify if:

  • \. Male and female children of all races with ages between 6 and 10

You may not qualify if:

  • History of rickets
  • History of intestinal malabsorption (i.e. Cystic Fibrosis, Fat Malabsorption Syndrome, Crohn's Disease)
  • History of severe medical illness, including renal failure (decrease 2/3 kidney function)
  • Allergies to orange juice
  • Any medical conditions in which it is not advisable to receive two 8 oz. glasses of orange juice per day
  • History of Diabetes
  • Currently taking, or having taken less than one month prior to start of study, a prescription vitamin D
  • Unwilling to consent/assent to this trial

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Boston Medical Center

Boston, Massachusetts, 02118, United States

Location

MeSH Terms

Conditions

Vitamin D DeficiencyAvitaminosisVitamin E Deficiency

Interventions

Vitamin DVitamin EVitamin ACalcium

Condition Hierarchy (Ancestors)

Deficiency DiseasesMalnutritionNutrition DisordersNutritional and Metabolic Diseases

Intervention Hierarchy (Ancestors)

SecosteroidsSteroidsFused-Ring CompoundsPolycyclic CompoundsBenzopyransPyransHeterocyclic Compounds, 1-RingHeterocyclic CompoundsHeterocyclic Compounds, 2-RingHeterocyclic Compounds, Fused-RingRetinoidsCarotenoidsPolyenesAlkenesHydrocarbons, AcyclicHydrocarbonsOrganic ChemicalsCyclohexenesCyclohexanesCycloparaffinsHydrocarbons, AlicyclicHydrocarbons, CyclicTerpenesDiterpenesPigments, BiologicalBiological FactorsMetals, Alkaline EarthElementsInorganic ChemicalsMetalsBlood Coagulation Factors

Study Officials

  • Micahel F Holick, PhD, MD

    BUMC

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

June 25, 2012

First Posted

July 26, 2013

Study Start

January 1, 2005

Primary Completion

January 1, 2008

Study Completion

January 1, 2008

Last Updated

February 28, 2017

Record last verified: 2017-02

Locations