The Effect of a Short Term Exercise Schedule on Oral Iron Bio-availability and Iron Incorporation

NCT01730521 | Completed

• 1 other identifier: EK 2012-N-27
• Study Type: observational
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

Iron metabolism may undergo changes during exercise, with reductions in classical iron status markers due to a variety of postulated mechanism which include hemodilution, increased iron loss, hemolysis and increased iron storage in muscles. Furthermore, it has been reported that vigorous training increases hepcidin, a central regulatory peptide in iron metabolism. This increase has been ascribed to the presence of subclinical inflammation. Increased hepcidin levels may reduce iron bioavailability and iron incorporation in erythrocytes. Twenty healthy men subjects will be recruited as subjects for this study. Subjects should be generally healthy, with no history of blood donation in the last 6 months, should weigh less than 85 Kg, and not take iron supplements and/or multivitamin supplements. Subjects should have familiarity to sports and running, but not currently (i.e. in the past 3 months) training for more than 1h per week on average. The aim of this study is to measure an iron bioavailability during a resting and an exercise phase lasting approx. 14 days with training sessions on alternate days. Subjects will participate in both restign and exercising protocols and act as their own controls during the study. Iron bioavailability will be measured via the incorporation of stable isotopic labels 14 days after administration. To control for changes in blood volume during the course of the study, blood volume of the participating subjects will be measured before and after the exercise phase with the CO-rebreathing method. Measurement of iron bioavailability and iron incorporation in a resting and exercising phase will allow determine if the increased level of hepcidin seen in in exercise will induce a lower iron bioavailability and iron incorporation during exercise.

Conditions

Generally Healthy

Outcome Measures

Primary Outcomes (1)

• Iron bioavailability from Stable isotopic labels

  Up to 2 months

Secondary Outcomes (1)

• Blood volume increase during exercise

  Up to 2 months

Other Outcomes (1)

• Hepcidin levels and inflammation markers (C-Reactive Protein, 1-Alpha acid glycoprotein)

  Up to 2 months

Study Arms (1)

Difference in Iron bioavailabilty exercise and resting phase

the subjects will act as their own control during the study

Behavioral: Exercise (Running)

Interventions

Exercise (Running) BEHAVIORAL

the study foresees a measurement of iron biavailability in a resting and in a exercising phase and subjects will act as their own control during the study.

Difference in Iron bioavailabilty exercise and resting phase

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Subjects studying or working at the University of Zürich or ETH Zürich

You may qualify if:

• men, Generally Healthy age between 18-50 years;
• BMI between 18-25;
• nonanemic (Hb \< 120 g/L);
• no intake of vitamins and nutritional supplements;
• no recent blood donation (\<4 months);
• no previous participation in studies with stable iron isotopes in the past.

You may not qualify if:

• Chronic diseases, Metabolic diseases, GI tract diseases (self reported)

Sponsors & Collaborators

• Swiss Federal Institute of Technology: lead

Study Sites (1)

ETH Zürich

Zurich, 8092, Switzerland

Location

Related Publications (2)

• Cercamondi CI, Egli IM, Ahouandjinou E, Dossa R, Zeder C, Salami L, Tjalsma H, Wiegerinck E, Tanno T, Hurrell RF, Hounhouigan J, Zimmermann MB. Afebrile Plasmodium falciparum parasitemia decreases absorption of fortification iron but does not affect systemic iron utilization: a double stable-isotope study in young Beninese women. Am J Clin Nutr. 2010 Dec;92(6):1385-92. doi: 10.3945/ajcn.2010.30051. Epub 2010 Oct 6.

  PMID: 20926522 BACKGROUND

• Moretti D, Mettler S, Zeder C, Lundby C, Geurts-Moetspot A, Monnard A, Swinkels DW, Brittenham GM, Zimmermann MB. An intensified training schedule in recreational male runners is associated with increases in erythropoiesis and inflammation and a net reduction in plasma hepcidin. Am J Clin Nutr. 2018 Dec 1;108(6):1324-1333. doi: 10.1093/ajcn/nqy247.

  PMID: 30351387 DERIVED

Biospecimen

Retention: SAMPLES WITH DNA

Whole blood and serum samples

MeSH Terms

Interventions

Exercise; Running

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Locomotion

Study Officials

• Diego Moretti, PhD

  ETH Zürich

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor, MD

Study Record Dates

• First Submitted: November 7, 2012
• First Posted: November 21, 2012
• Study Start: October 1, 2012
• Primary Completion: December 1, 2012
• Study Completion: June 1, 2013
• Last Updated: September 13, 2013

Record last verified: 2013-09

Locations

CH (1)