NCT01668836

Brief Summary

Women have a natural protection that gives them greater longevity. One hypothesis most commonly used is the estrogen protection in the premenopausal period. However many studies of various forms of hormone replacement therapy proved ineffective in promoting additional protection for women. Thus, it is discussed other ways of protection associated with longevity in women. Of these, the sirtuin system was found in several animal studies to be associated with longevity. This system also showed, through the involvement of several metabolic pathways, an important protection against the process of atherosclerosis. But the activity of this system in humans is unknown and if it is more active in women than in men. The study's main objectives are to analyze this system in healthy 24 women and 24 men aged 55 to 65 years, and their influence on the main metabolic pathways related to longevity and the process of atherosclerosis. The research protocol includes analysis of the influence of sirtuin (SIRT1) in vascular reactivity, lipid profile, antioxidant capacity, markers of inflammation and homeostasis, before and after the interventions with caloric restriction or resveratrol administration. It is expected of this study mechanistic conclusion for longevity and possible clinical applications in the mechanism of atherosclerosis prevention.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
48

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Aug 2012

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

August 1, 2012

Completed
13 days until next milestone

First Submitted

Initial submission to the registry

August 14, 2012

Completed
6 days until next milestone

First Posted

Study publicly available on registry

August 20, 2012

Completed
1.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 1, 2014

Completed
5 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2014

Completed
1.4 years until next milestone

Results Posted

Study results publicly available

April 18, 2016

Completed
Last Updated

April 18, 2016

Status Verified

March 1, 2016

Enrollment Period

1.9 years

First QC Date

August 14, 2012

Results QC Date

August 24, 2015

Last Update Submit

March 17, 2016

Conditions

Keywords

sirtuinatherosclerosislongevitycalorie restrictionresveratrolvascular reactivity

Outcome Measures

Primary Outcomes (2)

  • Direct Evaluation of the Sirtuin 1 Gene Expression

    The Sirtuin 1 gene expression was measured by real time PCR in peripheric blood. Unit of measure was arbitrary unit. "Arbitrary unit was relative to the control gene expression (control gene = 1)".

    30 days post-treatment

  • Sirtuin

    Sirtuin plasma levels before and 30 days post-treatment

    30 days post-treatment

Secondary Outcomes (10)

  • Influence of the Sirtuin 1 System on Lipid Profile, Glucose, and C-reactive Protein.

    30 days post-treatment

  • Influence of the Sirtuin 1 System on Apolipoproteins AI and B.

    30 days post-treatment

  • Influence of the Sirtuin 1 System on Estradiol

    30 days post-treatment

  • Influence of the Sirtuin 1 System on Platelet Aggregation.

    30 days post-treatment

  • Influence of the Sirtuin 1 System on Biomarkers

    30 days post-treatment

  • +5 more secondary outcomes

Other Outcomes (1)

  • Differences Between Men and Women.

    30 days

Study Arms (4)

men with resveratrol

EXPERIMENTAL

12 men will receive a pill with 500mg of resveratrol daily for 30 days

Drug: Resveratrol

women with resveratrol

EXPERIMENTAL

12 women will receive a pill with 500mg of resveratrol daily for 30 days

Drug: Resveratrol

men with caloric restriction

ACTIVE COMPARATOR

12 men will follow a 1000 calories per day diet for 30 days

Behavioral: Caloric restriction

women with caloric restriction

ACTIVE COMPARATOR

12 women will follow a 1000 calories per day diet for 30 days

Behavioral: Caloric restriction

Interventions

1 pill daily containing 500 mg/d of resveratrol for 30 days

men with resveratrolwomen with resveratrol

Diet of 1000kcal per day for 30 days

men with caloric restrictionwomen with caloric restriction

Eligibility Criteria

Age55 Years - 65 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • clinical history, physical examination and electrocardiogram normal

You may not qualify if:

  • premenopausal women (less than 1 year of natural amenorrhea)
  • Body mass index ≥ 35 kg/m2
  • Smoking
  • Severe hypertension (diastolic blood pressure ≥ 110 mmHg)
  • Severe dyslipidemia (triglycerides ≥ 500mg/dL, total cholesterol ≥ 300mg/dL)
  • Diabetes

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

INCOR - Heart Institute

São Paulo, São Paulo, 05403-900, Brazil

Location

MeSH Terms

Conditions

Vascular System InjuriesLipid Metabolism DisordersAtherosclerosis

Interventions

ResveratrolCaloric Restriction

Condition Hierarchy (Ancestors)

Vascular DiseasesCardiovascular DiseasesWounds and InjuriesMetabolic DiseasesNutritional and Metabolic DiseasesArteriosclerosisArterial Occlusive Diseases

Intervention Hierarchy (Ancestors)

StilbestrolsStilbenesBenzylidene CompoundsBenzene DerivativesHydrocarbons, AromaticHydrocarbons, CyclicHydrocarbonsOrganic ChemicalsPolyphenolsPhenolsDiet TherapyNutrition TherapyTherapeuticsEnergy IntakeDietNutritional Physiological PhenomenaDiet, Food, and NutritionPhysiological Phenomena

Results Point of Contact

Title
Antonio de Padua Mansur - Associate Professor
Organization
Heart Institute (InCor), University of São Paulo Medical School

Study Officials

  • Antônio de Pádua Mansur, PHD

    InCor Heart Institute

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
PHD

Study Record Dates

First Submitted

August 14, 2012

First Posted

August 20, 2012

Study Start

August 1, 2012

Primary Completion

July 1, 2014

Study Completion

December 1, 2014

Last Updated

April 18, 2016

Results First Posted

April 18, 2016

Record last verified: 2016-03

Locations