NCT01667341

Brief Summary

Randomized, double-blind, placebo-controlled, dose escalation study. There will be 3 cohorts of patients defined by the antigen dose (10, 30 or 100 µg of each antigen), and within each cohort, patients will be randomized at a ratio of 3:1:1 to one of the following:

  • 10 µg Cohort: 10 Pilot Group, 40 Continuation Group (50 Total)
  • 30 µg Cohort: 10 Pilot Group, 40 Continuation Group (50 Total)
  • 100 µg Cohort: 10 Pilot Group, 40 Continuation Group (50 Total)
  • Totals per group: 30 Pilot Group, 120 Continuation Group (150 Total Patients) Subjects will receive 3 doses of the assigned treatment (GEN-003/M-2, GEN-003, or placebo) at 3 week intervals. Sampling from mucocutaneous genital sites for viral shedding will be done twice daily for 28 days prior to the first immunization (baseline shedding), and again following the last immunization. Follow-up for safety monitoring will be conducted for 12 months after the last immunization.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
143

participants targeted

Target at P75+ for phase_1

Timeline
Completed

Started Jul 2012

Geographic Reach
1 country

7 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

July 1, 2012

Completed
1 month until next milestone

First Submitted

Initial submission to the registry

August 14, 2012

Completed
3 days until next milestone

First Posted

Study publicly available on registry

August 17, 2012

Completed
1.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 9, 2014

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

May 9, 2014

Completed
Last Updated

June 1, 2018

Status Verified

May 1, 2018

Enrollment Period

1.9 years

First QC Date

August 14, 2012

Last Update Submit

May 30, 2018

Conditions

Keywords

HSVHerpesgenital infectionvaccine

Outcome Measures

Primary Outcomes (1)

  • Number of patients with adverse events as a measure of safety and tolerability

    57 Weeks

Secondary Outcomes (2)

  • Immunogenicity measured by humoral (antibody) and T-cell responses to vaccine antigens

    33 weeks

  • Change in proportion of days with detectable viral shedding

    6 weeks

Study Arms (7)

Low Dose GEN-003 with Matrix M-2

EXPERIMENTAL

10µg GEN-003, 50µg Matrix M-2 Adjuvant

Biological: GEN-003 with Matrix M-2

Mid Dose GEN-003 with Matrix M-2

EXPERIMENTAL

30µg GEN-003, 50µg Matrix M-2 Adjuvant

Biological: GEN-003 with Matrix M-2

High Dose GEN-003 with Matrix M-2

EXPERIMENTAL

100µg GEN-003, 50µg Matrix M-2 Adjuvant

Biological: GEN-003 with Matrix M-2

Low Dose GEN-003 Only

EXPERIMENTAL

10µg GEN-003

Biological: GEN-003

Mid Dose GEN-003 Only

EXPERIMENTAL

30µg GEN-003

Biological: GEN-003

High Dose GEN-003 Only

EXPERIMENTAL

100µg GEN-003

Biological: GEN-003

Placebo

PLACEBO COMPARATOR

0.5 mL phosphate buffered saline

Biological: Placebo

Interventions

IM administration of GEN-003 Vaccine with 50ug Matrix M-2 Adjuvant.

High Dose GEN-003 with Matrix M-2Low Dose GEN-003 with Matrix M-2Mid Dose GEN-003 with Matrix M-2
GEN-003BIOLOGICAL

IM administration of GEN-003 Vaccine, antigens alone (without adjuvant).

High Dose GEN-003 OnlyLow Dose GEN-003 OnlyMid Dose GEN-003 Only
PlaceboBIOLOGICAL

IM administration of 0.5 mL dose of Dulbecco's phosphate buffered saline.

Also known as: PBS, DPBS
Placebo

Eligibility Criteria

Age18 Years - 50 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • Men and women ages 18 to 50 years, inclusive.
  • Willing to practice a highly effective method of contraception that includes the use of a barrier method such as a condom.
  • Diagnosis of genital HSV-2 infection for \> 1 year supported by ONE of the following documented in the medical history or performed at screening:
  • Western blot for HSV-2
  • Type-specific polymerase chain reaction (PCR) or viral culture
  • Compatible clinical history AND HSV-2 ELISA (HerpSelect) index value \>3.5
  • A history of at least 3 and no more than 9 reported clinical occurrences in the prior 12 months, or, if currently on suppressive therapy, history of at least 3 and no more than 9 reported clinical occurrences in the 12 months prior to initiation suppressive therapy.
  • Good general health status as determined by screening evaluation completed within 90 days prior to immunization. Any out of range screening clinical laboratory values should be considered not clinically significant by the Investigator.
  • Patient has provided written informed consent.
  • Ability and willingness to perform and comply with all study procedures including attending clinic visits as scheduled. Note: patients must provide, by the day of randomization, a minimum of 28 (equivalent of 14 days) baseline viral swab samples to continue to be eligible and be randomized).

You may not qualify if:

  • On suppressive antiviral medication within 7 days of baseline viral shedding evaluation.
  • Immunocompromised individuals, including those receiving systemic corticosteroids or other immunosuppressive agents.
  • Positive serologic test for HIV-1 infection; positive hepatitis B surface antigen (HBsAg) or antibody for hepatitis C (anti-HCV).
  • Active lesions consistent with herpetic disease at the time of scheduled immunization.
  • Pregnant or nursing women.
  • Receipt of any investigational drug within 30 days of the first scheduled day of immunization.
  • History of hypersensitivity to any component of the vaccine.
  • History of genital HSV-1 infection.
  • History of: (1) any form of ocular HSV infection, (2) HSV-related erythema multiforme, or (3) herpes meningitis or encephalitis.
  • Any other condition which in the opinion of the Investigator would interfere with the successful completion of the study protocol.
  • History of drug or alcohol abuse that, in the opinion of the Investigator, would interfere with the patient's ability to comply with the requirements of the study.
  • Prior immunization with a vaccine containing HSV-2 antigens.
  • Receipt of blood products within 90 days of the first immunization.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (7)

University of Alabama Vaccine Research Unit

Birmingham, Alabama, 35294-0006, United States

Location

Indiana University Infectious Disease Research

Indianapolis, Indiana, 46202, United States

Location

Cincinnati Children's Hospital Medical Center

Cincinnati, Ohio, 45229-3039, United States

Location

Westover Heights Clinic

Portland, Oregon, 97210, United States

Location

Center for Clinical Studies - Houston

Houston, Texas, 77030, United States

Location

Center for Clinical Studies - Clear Lake/Webster

Webster, Texas, 77598, United States

Location

UW Virology Research Clinic

Seattle, Washington, 98104, United States

Location

Related Publications (1)

  • Bernstein DI, Wald A, Warren T, Fife K, Tyring S, Lee P, Van Wagoner N, Magaret A, Flechtner JB, Tasker S, Chan J, Morris A, Hetherington S. Therapeutic Vaccine for Genital Herpes Simplex Virus-2 Infection: Findings From a Randomized Trial. J Infect Dis. 2017 Mar 15;215(6):856-864. doi: 10.1093/infdis/jix004.

MeSH Terms

Conditions

Herpes Simplex

Condition Hierarchy (Ancestors)

Herpesviridae InfectionsDNA Virus InfectionsVirus DiseasesInfectionsSkin Diseases, ViralSkin Diseases, InfectiousSkin DiseasesSkin and Connective Tissue Diseases

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

August 14, 2012

First Posted

August 17, 2012

Study Start

July 1, 2012

Primary Completion

May 9, 2014

Study Completion

May 9, 2014

Last Updated

June 1, 2018

Record last verified: 2018-05

Locations