NCT01589991

Brief Summary

Fluoride toothpaste has been associated not only with declining dental caries prevalence but also with an increase in dental fluorosis. In the balance of benefits/risks of fluoride toothpaste use, the purpose of this study is to evaluate the availability of fluoride concentration in the mouth (biofilm fuild), as an indicator of fluoride benefits (anticaries effect), and the fluoride concentration in urine, as an indicator of fluoride absorption from ingested toothpastes (with potential to cause dental fluorosis), in a sample of young Brazilian children using toothpaste formulations representative of those available and most used by this age-group.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
24

participants targeted

Target at P25-P50 for phase_1

Timeline
Completed

Started Jan 2011

Typical duration for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 1, 2011

Completed
1.3 years until next milestone

First Submitted

Initial submission to the registry

April 30, 2012

Completed
2 days until next milestone

First Posted

Study publicly available on registry

May 2, 2012

Completed
7 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2012

Completed
7 months until next milestone

Study Completion

Last participant's last visit for all outcomes

July 1, 2013

Completed
Last Updated

October 28, 2013

Status Verified

October 1, 2013

Enrollment Period

1.9 years

First QC Date

April 30, 2012

Last Update Submit

October 25, 2013

Conditions

Keywords

toothpastesbioavailabilitysodium fluoridemonofluorphosphatelow-fluoride

Outcome Measures

Primary Outcomes (2)

  • Fluoride concentration in biofilm fluid

    Remants of dental plaque (biofilm) will be collected 30 min after brushing with the toothpastes, the fluid will be separated from the solids by centrifugation and the fluoride concentration in the biofilm fluid will be determined by a fluoride electrode adapted for microanalysis.

    30min

  • Urinary fluoride excretion

    Urine will be collected at two time-points, either before the use of the toothpaste or at the last day of use, in each experimental phase. Fluoride concentration was determined by an inverted ion-specific fluoride electrode and fluoride excretion will be measured by fluoride/cratinine ratio (mg F/mg creatinine).

    8h

Secondary Outcomes (3)

  • Fluoride concentration in biofilm solid

    30min

  • Fluoride concentration in saliva

    30min

  • Amount of toothpaste ingested

    January 2013 to July 2013

Study Arms (4)

Non-fluoride toothpaste

PLACEBO COMPARATOR
Drug: Toothpaste

Toothpaste 550 µg F/g (NaF/SiO2)

EXPERIMENTAL
Drug: Toothpaste

Toothpaste 1100 µg F/g (NaF/SiO2)

EXPERIMENTAL
Drug: Toothpaste

Toothpaste 1450 µg F/g (MFP/CaCO3)

EXPERIMENTAL
Drug: Toothpaste

Interventions

The volunteers brushed their teeth with 0.3 g of the assigned toothpastes, 3x/day.

Non-fluoride toothpasteToothpaste 1100 µg F/g (NaF/SiO2)Toothpaste 1450 µg F/g (MFP/CaCO3)Toothpaste 550 µg F/g (NaF/SiO2)

Eligibility Criteria

Age3 Years - 4 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17)

You may qualify if:

  • Good general health
  • Good oral health

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Piracicaba Dental School, University of Campinas

Piracicaba, São Paulo, 13414903, Brazil

Location

Related Publications (8)

  • Cury JA, do Amaral RC, Tenuta LM, Del Bel Cury AA, Tabchoury CP. Low-fluoride toothpaste and deciduous enamel demineralization under biofilm accumulation and sucrose exposure. Eur J Oral Sci. 2010 Aug;118(4):370-5. doi: 10.1111/j.1600-0722.2010.00745.x.

    PMID: 20662910BACKGROUND
  • Lima TJ, Ribeiro CC, Tenuta LM, Cury JA. Low-fluoride dentifrice and caries lesion control in children with different caries experience: a randomized clinical trial. Caries Res. 2008;42(1):46-50. doi: 10.1159/000111749. Epub 2007 Nov 27.

    PMID: 18042987BACKGROUND
  • Cury JA, Tenuta LM, Ribeiro CC, Paes Leme AF. The importance of fluoride dentifrices to the current dental caries prevalence in Brazil. Braz Dent J. 2004;15(3):167-74. doi: 10.1590/s0103-64402004000300001. Epub 2005 Mar 18.

    PMID: 15798817BACKGROUND
  • Paes Leme AF, Dalcico R, Tabchoury CP, Del Bel Cury AA, Rosalen PL, Cury JA. In situ effect of frequent sucrose exposure on enamel demineralization and on plaque composition after APF application and F dentifrice use. J Dent Res. 2004 Jan;83(1):71-5. doi: 10.1177/154405910408300114.

    PMID: 14691117BACKGROUND
  • Cury JA, Francisco SB, Simoes GS, Del Bel Cury AA, Tabchoury CP. Effect of a calcium carbonate-based dentifrice on enamel demineralization in situ. Caries Res. 2003 May-Jun;37(3):194-9. doi: 10.1159/000070444.

    PMID: 12740543BACKGROUND
  • Cury JA, Simoes GS, Del Bel Cury AA, Goncalves NC, Tabchoury CP. Effect of a calcium carbonate-based dentifrice on in situ enamel remineralization. Caries Res. 2005 May-Jun;39(3):255-7. doi: 10.1159/000084807.

    PMID: 15914990BACKGROUND
  • Cury JA, Oliveira MJ, Martins CC, Tenuta LM, Paiva SM. Available fluoride in toothpastes used by Brazilian children. Braz Dent J. 2010;21(5):396-400. doi: 10.1590/s0103-64402010000500003.

    PMID: 21180793BACKGROUND
  • Martins CC, Oliveira MJ, Pordeus IA, Cury JA, Paiva SM. Association between socioeconomic factors and the choice of dentifrice and fluoride intake by children. Int J Environ Res Public Health. 2011 Nov;8(11):4284-99. doi: 10.3390/ijerph8114284. Epub 2011 Nov 10.

    PMID: 22163207BACKGROUND

MeSH Terms

Conditions

Dental CariesFluorosis, Dental

Interventions

Toothpastes

Condition Hierarchy (Ancestors)

Tooth DemineralizationTooth DiseasesStomatognathic DiseasesDental Enamel HypomineralizationDevelopmental Defects of EnamelTooth AbnormalitiesStomatognathic System AbnormalitiesCongenital AbnormalitiesCongenital, Hereditary, and Neonatal Diseases and Abnormalities

Intervention Hierarchy (Ancestors)

DentifricesBiomedical and Dental MaterialsManufactured MaterialsTechnology, Industry, and Agriculture

Study Officials

  • Livia MA Tenuta, PhD

    Piracicaba Dental School, University of Campinas

    STUDY CHAIR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
INVESTIGATOR
Purpose
PREVENTION
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assistant Professor, Biochemistry and Cariology

Study Record Dates

First Submitted

April 30, 2012

First Posted

May 2, 2012

Study Start

January 1, 2011

Primary Completion

December 1, 2012

Study Completion

July 1, 2013

Last Updated

October 28, 2013

Record last verified: 2013-10

Locations