Safety and Preliminary Efficacy of the Malaria Vaccine Candidates Falciparum Merozoite Protein-1 (FMP1) and SmithKlineBeecham (SKBB) Candidate Malaria Vaccine RTS,S

NCT01556945 | Completed Phase 1

• 1 other identifier: WRAIR #849
• Study Type: interventional
• Target: 72
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to see if two new malaria vaccines called FMP1 and RTSS, combined with an adjuvant (called SBAS2) which helps stimulate the body's immune system, are safe, demonstrate an immune response through blood tests, and lastly, to see if the vaccines can prevent malaria infection. The RTS,S vaccine contains a malaria protein in combination with a portion of the commercially available hepatitis B vaccine. The FMP1 vaccine also contains a malaria protein. The adjuvant called SBAS2, is a special oil in water emulsion. Vaccinations are done at study days 0, 28 and 84, followed by a malaria challenge approximately 14 days after the 3rd vaccination.

Conditions

Malaria, Falciparum

Keywords

P. falciparum; vaccine; healthy volunteers

Outcome Measures

Primary Outcomes (1)

• Safety

  Measured through adverse event collection and immunogenicity results

  two years

Study Arms (5)

Group A : FMP1/AS02 + RTS,S/AS02

EXPERIMENTAL

FMP1 malaria vaccine given with the GlaxoSmithKline (GSK) adjuvant system, number 2 (AS02) and a second experimental malaria vaccine RTS,S also given with AS02 adjuvant concomitantly as separate sites of injection on days 0, 28 and 84. Malaria challenge phase began 14-30 days after the last vaccine.

Biological: FMP1/AS02; Biological: RTS,S/AS02; Other: Malaria challenge

Group B : FMP1/AS02 + RTS,S/AS02

EXPERIMENTAL

FMP1 malaria vaccine given with the adjuvant AS02 and a second experimental malaria vaccine RTS,S also given with AS02 adjuvant at one injection site and saline at the opposite site on days 0, 28 and 84. Malaria challenge phase began 14-30 days after the last vaccine.

Biological: FMP1/AS02; Biological: RTS,S/AS02; Other: Malaria challenge

Group C: FMP1/AS02 + AS02

EXPERIMENTAL

FMP1 malaria vaccine given with the adjuvant AS02 and a second experimental malaria vaccine RTS,S also given with adjuvant AS02 adjuvant alone at one injection site and saline at the opposite site on days 0, 28 and 84. Malaria challenge phase began 14-30 days after the last vaccine.

Biological: FMP1/AS02; Other: AS02 adjuvant alone; Other: Malaria challenge

Group D : RTS,S/AS02 + AS02

EXPERIMENTAL

RTS,S malaria vaccine given with the adjuvant AS02 and an adjuvant AS02 alone concomitantly at separate sites of injection on days 0, 28 and 84. Malaria challenge phase began 14-30 days after the last vaccine.

Biological: RTS,S/AS02; Other: AS02 adjuvant alone; Other: Malaria challenge

Control cohort

PLACEBO COMPARATOR

Infectivity controls (unvaccinated). Non-randomized infectivity controls were recruited specifically for the malaria challenge phase of the trial.

Other: Malaria challenge

Interventions

FMP1/AS02 BIOLOGICAL

The vaccine antigen FMP1 consists of a recombinant histidine-tagged (His6) fusion protein expressed in E. coli. The lyophilized pellet contained per vaccine vial 62.5 μg merozoite surface protein-142 (MSP-142) with 3.1% lactose as cryoprotectant in each 3 ml monodose vial. The pellet was reconstituted with AS02.

Group A : FMP1/AS02 + RTS,S/AS02; Group B : FMP1/AS02 + RTS,S/AS02; Group C: FMP1/AS02 + AS02

RTS,S/AS02 BIOLOGICAL

The vaccine antigen RTS,S, is a recombinant subunit vaccine produced in, and purified from yeast cells. The final lyophilized pellet contained 62.5 μg RTS,S with 3.15% lactose as cryoprotectant per 3 ml monodose vial. The pellet was reconstituted in AS02 and each 0.5 ml dose contained 50 μg RTS,S.

Group A : FMP1/AS02 + RTS,S/AS02; Group B : FMP1/AS02 + RTS,S/AS02; Group D : RTS,S/AS02 + AS02

AS02 adjuvant alone OTHER

AS02 adjuvant contains 50 μg monophosphoryl lipid A (MPL) and 50 μg Quillaja saponaria 21 (QS-21), 250 μl of SB62 (oil/water emulsion) in phosphate buffered saline (PBS) per volume of 0.5 ml.

Group C: FMP1/AS02 + AS02; Group D : RTS,S/AS02 + AS02

Malaria challenge OTHER

Experimental challenge homologous strain of P.falciparum sporozoites. Mosquitoes infected with malaria approximately 17 to 19 days earlier and that contained sporozoites in their salivary glands. For each volunteer, five mosquitoes were allowed to feed over five minutes, after which they were dissected to confirm how many were infected, and the salivary glands scored.

Control cohort; Group A : FMP1/AS02 + RTS,S/AS02; Group B : FMP1/AS02 + RTS,S/AS02; Group C: FMP1/AS02 + AS02; Group D : RTS,S/AS02 + AS02

Eligibility Criteria

• Age: 18 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy adult, 18-45
• Available for duration of study (9 months)
• Written informed consent prior to any study procedures

You may not qualify if:

• Prior receipt of an investigational malaria vaccine or one containing MPL or QS-21
• Use of any investigational or non-registered drug/vaccine or planned administration of vaccine not foreseen by study protocol; each issue within 30 days preceding the first dose of study vaccine
• Administration of chronic immunosuppressants
• Chronic use of antibiotics
• History of malaria ever, or use of malaria chemoprophylaxis within 60 days prior to vaccination
• Known exposure to malaria within the past 12 months or planned travel to malarious area during the study period
• Confirmed or suspected immunosuppressive or immunodeficient condition
• Family history of congenital or hereditary immunodeficiency
• History of allergic disease or reactions likely to be exacerbated by any component of the vaccine
• Chronic or active neurologic disease including seizures
• History of splenectomy
• Seropositive for hepatitis B or hepatitis C or Human Immunodeficiency Virus (HIV), or other abnormal labs such as significant anemia, elevated creatinine
• Hepatomegaly, or right upper quadrant abdominal pain
• Pregnant or lactating female
• Chronic or active drug or alcohol use

Sponsors & Collaborators

• U.S. Army Medical Research and Development Command: lead
• GlaxoSmithKline: collaborator

Study Sites (1)

WRAIR Clinical Trials Center

Silver Spring, Maryland, 20910, United States

Location

Related Publications (1)

• Cummings JF, Polhemus ME, Kester KE, Ockenhouse CF, Gasser RA Jr, Coyne P, Wortmann G, Nielsen RK, Schaecher K, Holland CA, Krzych U, Tornieporth N, Soisson LA, Angov E, Heppner DG; RTS,S Vaccine Evaluation Group. A phase IIa, randomized, double-blind, safety, immunogenicity and efficacy trial of Plasmodium falciparum vaccine antigens merozoite surface protein 1 and RTS,S formulated with AS02 adjuvant in healthy, malaria-naive adults. Vaccine. 2024 Apr 30;42(12):3066-3074. doi: 10.1016/j.vaccine.2024.03.072. Epub 2024 Apr 6.

  PMID: 38584058 DERIVED

MeSH Terms

Conditions

Malaria, Falciparum

Condition Hierarchy (Ancestors)

Malaria; Protozoan Infections; Parasitic Diseases; Infections; Mosquito-Borne Diseases; Vector Borne Diseases

Study Design

• Study Type: interventional
• Phase: phase 1
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: PREVENTION
• Intervention Model: FACTORIAL
• Sponsor Type: FED
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 14, 2012
• First Posted: March 19, 2012
• Study Start: April 1, 2001
• Primary Completion: February 1, 2002
• Last Updated: May 2, 2014

Record last verified: 2014-05

Locations

US (1)