NCT01530893

Brief Summary

The primary aim of this placebo-controlled study is to determine whether flavonoids beneficially affect markers of cardiovascular disease risk in healthy subjects at elevated cardiovascular risk. The underlying mechanisms of action of flavonoids will be assessed on blood biomarkers and we will assess the effects of the food matrix, and aspects of isoflavone metabolism.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
64

participants targeted

Target at P50-P75 for not_applicable

Timeline
Completed

Started Feb 2012

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

February 1, 2012

Completed
Same day until next milestone

Study Start

First participant enrolled

February 1, 2012

Completed
9 days until next milestone

First Posted

Study publicly available on registry

February 10, 2012

Completed
1.8 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2013

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2013

Completed
Last Updated

February 28, 2014

Status Verified

February 1, 2014

Enrollment Period

1.8 years

First QC Date

February 1, 2012

Last Update Submit

February 27, 2014

Conditions

Keywords

flavonoidsvascular functionrandomised controlled trialplacebo controlled

Outcome Measures

Primary Outcomes (1)

  • Change from baseline in blood vessel control (pressure and endothelial function) at time points coinciding with postprandial [anticipated] peak plasma flavonoid concentration.

    Assessment of vascular function

    acute postprandial; up to 24 hours, dependent on flavonoid sub-classification studied

Secondary Outcomes (1)

  • Differences in effects of flavonoids introduced by food matrix

    acute postprandial; up to 24 hours dependent on flavonoid sub-classification studied

Study Arms (4)

Intervention group A: flavanones

EXPERIMENTAL

All experimental days will be exactly the same. All volunteers will attend the clinical research and trial unit in a fasted state. Prior to test administration, baseline vascular function will be assessed and biological samples taken by a trained and qualified research nurse. Subsequently, assessments will be repeated at times corresponding to anticipated peak plasma concentration of the flavonoid of interest.

Dietary Supplement: Flavanone - supplementOther: Flavanone - foodOther: Flavanone - placebo

Intervention group B: isoflavones

EXPERIMENTAL

All experimental days will be exactly the same. All volunteers will attend the clinical research and trial unit in a fasted state. Prior to test administration, baseline vascular function will be assessed and biological samples taken by a trained and qualified research nurse. Subsequently, assessments will be repeated at times corresponding to anticipated peak plasma concentration of the flavonoid of interest.

Dietary Supplement: Isoflavone - supplementOther: Isoflavone - foodOther: Isoflavone - placeboDietary Supplement: Isoflavone - metabolite supplement

Intervention group C: Flavan-3-ols

EXPERIMENTAL

All experimental days will be exactly the same. All volunteers will attend the clinical research and trial unit in a fasted state. Prior to test administration, baseline vascular function will be assessed and biological samples taken by a trained and qualified research nurse. Subsequently, assessments will be repeated at times corresponding to anticipated peak plasma concentration of the flavonoid of interest.

Dietary Supplement: Flavan-3-ol - supplementOther: Flavan-3-ol - foodOther: Flavan-3-ol - placebo

Intervention group D: Anthocyanins

EXPERIMENTAL

All experimental days will be exactly the same. All volunteers will attend the clinical research and trial unit in a fasted state. Prior to test administration, baseline vascular function will be assessed and biological samples taken by a trained and qualified research nurse. Subsequently, assessments will be repeated at times corresponding to anticipated peak plasma concentration of the flavonoid of interest.

Dietary Supplement: Anthocyanin - supplementOther: Anthocyanin - foodOther: Anthocyanin - placebo

Interventions

Flavanone - supplementDIETARY_SUPPLEMENT

acute single optimal dose

Intervention group A: flavanones

acute single dose

Intervention group A: flavanones

acute single dose (flavanone free)

Intervention group A: flavanones
Isoflavone - supplementDIETARY_SUPPLEMENT

acute single optimal dose

Intervention group B: isoflavones

acute single optimal dose

Intervention group B: isoflavones

acute single dose (isoflavone free)

Intervention group B: isoflavones

acute single optimal dose of commercial product

Intervention group B: isoflavones
Flavan-3-ol - supplementDIETARY_SUPPLEMENT

acute single optimal dose

Intervention group C: Flavan-3-ols

acute single optimal dose

Intervention group C: Flavan-3-ols

acute single dose (flavan-3-ol free)

Intervention group C: Flavan-3-ols
Anthocyanin - supplementDIETARY_SUPPLEMENT

acute single optimal dose

Intervention group D: Anthocyanins

acute single optimal dose

Intervention group D: Anthocyanins

acute single dose (anthocyanin free)

Intervention group D: Anthocyanins

Eligibility Criteria

Age50 Years - 75 Years
Sexmale
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Males
  • to 75 years old
  • At elevated risk of CV disease
  • Screening blood pressure of \< 160/90mmHg (at rest)
  • Successful biochemical, haematological and urinalysis assessment at screening (as judged by clinical advisor)

You may not qualify if:

  • Current smokers, or ex-smokers ceasing \< 3 months ago
  • Subjects with existing or significant past medical history of vascular disease or medical conditions likely to affect the study measures (as judged by clinical advisor)
  • Those with known allergies to the intervention treatments
  • Those unprepared to adhere to dietary restrictions during the trial
  • Parallel participation in another research project which involves concurrent dietary intervention and/or sampling of biological fluids/material
  • Having vaccinations (excluding the flu vaccination) or antibiotics within 3 months of start of trial, and those with vaccinations scheduled for during the trial
  • Taking flavonoid containing food / dietary supplements likely to affect the study results
  • Prescribed lipid lowering, hypertension, vasodilators (e.g. Viagra) or antibiotic medication
  • Unsatisfactory biochemical, haematological or urinary assessment at screening, indicating abnormal; renal or liver function, full blood profile, impaired glucose handling, deranged lipids or measurements considered to be counter indicative of the study by the clinical advisor

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Department of Nutrition, Norwich Medical School

Norwich, Norfolk, NR4 7TJ, United Kingdom

Location

Related Publications (2)

  • Hazim S, Curtis PJ, Schar MY, Ostertag LM, Kay CD, Minihane AM, Cassidy A. Acute benefits of the microbial-derived isoflavone metabolite equol on arterial stiffness in men prospectively recruited according to equol producer phenotype: a double-blind randomized controlled trial. Am J Clin Nutr. 2016 Mar;103(3):694-702. doi: 10.3945/ajcn.115.125690. Epub 2016 Feb 3.

  • Schar MY, Curtis PJ, Hazim S, Ostertag LM, Kay CD, Potter JF, Cassidy A. Orange juice-derived flavanone and phenolic metabolites do not acutely affect cardiovascular risk biomarkers: a randomized, placebo-controlled, crossover trial in men at moderate risk of cardiovascular disease. Am J Clin Nutr. 2015 May;101(5):931-8. doi: 10.3945/ajcn.114.104364. Epub 2015 Mar 18.

Study Officials

  • Aedin Cassidy, PhD

    University of East Anglia

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
BASIC SCIENCE
Intervention Model
FACTORIAL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

February 1, 2012

First Posted

February 10, 2012

Study Start

February 1, 2012

Primary Completion

December 1, 2013

Study Completion

December 1, 2013

Last Updated

February 28, 2014

Record last verified: 2014-02

Locations