Genetic and Physiological Aspects of Oxidative Profile in Sleep and Well-succeed Aging
1 other identifier
observational
38
1 country
1
Brief Summary
The present study proposes the characterization of sleep patterns of healthy young adults, elderly individuals and individuals above 85 years old, using polysomnographic recordings, in order to clarify the importance of sleep in longevity. Furthermore, this study intends to analyze the oxidative stress-related gene expression in peripheral blood of the three studied groups, using the Superarray - RT2 Profiler" PCR Array System. After the identification of genes whose expression pattern among groups suggest a more specific role in longevity, the mechanisms of gene expression regulation, including DNA methylation patterns and microRNA expression, as well as the possible genomic sources of variation in these genes will be investigated. In addition, the oldest individual (105 years-old) will have his whole genome sequenced using next-generation sequencing technology, in order to identify rare variants associated with longevity. Subsequently, the effect of the polymorphisms and rare variants identified will be confirmed in an expanded sample constituted of individuals with various age-ranges. The study will provide a better characterization of molecular and physiological mechanisms involved in longevity, hoping to contribute to the development of more advanced clinical tools, capable to offer a better quality of life for the elderly.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at P25-P50 for all trials
Started Mar 2010
Typical duration for all trials
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
March 1, 2010
CompletedPrimary Completion
Last participant's last visit for primary outcome
June 1, 2011
CompletedFirst Submitted
Initial submission to the registry
November 23, 2011
CompletedFirst Posted
Study publicly available on registry
November 28, 2011
CompletedStudy Completion
Last participant's last visit for all outcomes
February 1, 2013
CompletedJanuary 14, 2015
January 1, 2015
1.3 years
November 23, 2011
January 13, 2015
Conditions
Outcome Measures
Primary Outcomes (2)
Polysomnography parameters
1 night
Gene expression data
1 day
Study Arms (3)
Elderly
Individuals between 60 and 70 years-old
Long-lived
Individuals above 85 years-old
Young
Individuals between 20 and 30 years-old
Eligibility Criteria
Residents of São Paulo city, Brazil
You may qualify if:
- men aged between 20-30 years-old,
- years-old and above 90 years-old
You may not qualify if:
- Uncontrolled chronic disease (cancer, cardiopathy, type 2 diabetes)
- neurological and/or psychiatric antecedents
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (1)
Associação Fundo de Incentivo à Pesquisa
São Paulo, São Paulo, 04024002, Brazil
Biospecimen
Blood samples collected for DNA and RNA extraction, as well as for laboratory examinations
Study Officials
- STUDY DIRECTOR
Sergio Tufik, MD PhD
Associação Fundo de Incentivo à Pesquisa
Study Design
- Study Type
- observational
- Observational Model
- CASE CONTROL
- Time Perspective
- CROSS SECTIONAL
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Diego Robles Mazzotti
Study Record Dates
First Submitted
November 23, 2011
First Posted
November 28, 2011
Study Start
March 1, 2010
Primary Completion
June 1, 2011
Study Completion
February 1, 2013
Last Updated
January 14, 2015
Record last verified: 2015-01