Metabolic Availability of Lysine From Rice

NCT01474226 | Completed Phase 1

• 2 other identifiers: 0019850580CIHR, grant MT10321
• Study Type: interventional
• Target: 5
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to investigate the digestibility of the amino acid lysine in cooked rice in adult men using stable isotope technique.

Conditions

Healthy Adults

Keywords

lysine; rice; bioavailability

Outcome Measures

Primary Outcomes (4)

• Metabolic Availability of lysine

  3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

  Baseline

• Metabolic Availability of lysine

  3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

  15 min after intake of the fourth hourly meal (before tracer infusion))

• Metabolic Availability of lysine

  3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

  30 min after intake of the fourth hourly meal (before tracer infusion))

• Metabolic Availability of lysine

  3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

  45 min after intake of the fourth hourly meal (before tracer infusion))

Secondary Outcomes (1)

• Metabolic Availability of Lysine

  Post Tracer Infusion (between 150 and 240 min of tracer infusion)

Study Arms (1)

Lysine Amino Acid

EXPERIMENTAL

Dietary Supplement: Amino Acid Crystalline Lysine Amino Acid Mix; Dietary Supplement: Cooked Rice containing Lysine

Interventions

Amino Acid Crystalline Lysine Amino Acid Mix DIETARY_SUPPLEMENT

crystalline lysine from AA MIX (20, 40, and 60% of the mean lysine requirement of 35 mg/kg/d) Lysine will be given in the form of L-Lysine-HCl, Sigma-Aldrich Brand.

Lysine Amino Acid

Cooked Rice containing Lysine DIETARY_SUPPLEMENT

Lysine (20,40 and 60 % of the mean lysine requirement) from Cooked Rice

Lysine Amino Acid

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy Adult males (18 to 50years old)

You may not qualify if:

• A history of recent weight loss or illness
• Use of any medication at the time of entry into the study.

Sponsors & Collaborators

• The Hospital for Sick Children: lead

Study Sites (1)

The Hospital for Sick Children

Toronto, Ontario, M5G-1X8, Canada

Location

Related Publications (1)

• Prolla IR, Rafii M, Courtney-Martin G, Elango R, da Silva LP, Ball RO, Pencharz PB. Lysine from cooked white rice consumed by healthy young men is highly metabolically available when assessed using the indicator amino acid oxidation technique. J Nutr. 2013 Mar;143(3):302-6. doi: 10.3945/jn.112.166728. Epub 2013 Jan 16.

  PMID: 23325920 DERIVED

Study Officials

• Paul B Pencharz, MD, PhD

  The Hospital for Sick Children, Toronto, Canada

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: phase 1
• Allocation: NA
• Masking: NONE
• Purpose: HEALTH SERVICES RESEARCH
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor of Paediatrics and Nutritional Sciences (Emeritus) University of Toronto, Senior Scientist Research Institute,The Hospital for Sick Children

Study Record Dates

• First Submitted: November 10, 2011
• First Posted: November 18, 2011
• Study Start: January 1, 2011
• Primary Completion: November 1, 2011
• Study Completion: November 1, 2011
• Last Updated: July 29, 2013

Record last verified: 2013-07

Locations

CA (1)