Disturbances in BCAA Metabolism and the Effects of Feeding and Exercise in COPD

NCT01418469 | Completed

• 1 other identifier: MEC 02-059.3
• Study Type: interventional
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

Studies on resting human muscle show that ingestion of the branched-chain amino acids (BCAA): leucine, valine and isoleucine have an anabolic effect on muscle protein metabolism. However, the effects of BCAA intake on protein metabolism during exercise are less clear. When BCAA were supplied as single amino acids, without other amino acids and/or carbohydrates, no effects were observed on protein kinetics. On the other hand, ingestion of BCAA during running appeared to reduce the catabolic effect of running on muscle protein metabolism. These experiments were all performed with mixtures of the BCAA with or without carbohydrates but not in the form of complete meals with food protein as a basis. Therefore, it is still unknown whether a protein meal, containing a substantial amount of BCAA is beneficial during exercise by inducing an anabolic effect. Whey and Casein protein contain a substantial amount of BCAA in contrast to Soy protein. Therefore, it is hypothesized that milk-based proteins are a better and more physiological source of BCAA during exercise and will lead to more protein anabolism. Most of the available studies have been carried out in young and fit humans but there are hardly any data are available in the increasing population of the elderly. Therefore it is still unknown whether a BCAA rich protein meal can enhance the anabolic effect of exercise in older individuals. Besides sarcopenia, a substantial part of the elderly is suffering from a chronic systemic disease such as chronic obstructive pulmonary disease (COPD). COPD represents an important health care problem. COPD is the fourth leading cause of death and will be the third leading cause worldwide in 2020. Besides the local impairment, COPD is a chronic wasting disease, associated with alterations in intermediary metabolism. Substantial disturbances have been found in BCAA (and related) metabolism in these patients at rest and during exercise. It might therefore be of clinical relevance to study the metabolic effects of BCAA rich protein meals in patients with COPD at rest and during exercise.

Conditions

Chronic Obstructive Pulmonary Disease

Keywords

COPD; protein metabolism; branched-chain amino acid metabolism; exercise; protein feeding

Outcome Measures

Primary Outcomes (1)

• Change in Net whole body protein synthesis

  Net whole body protein synthesis during protein feeding and the response to a 20 min cycle exercise bout

  6 hours

Secondary Outcomes (9)

• Change in whole body protein synthesis rate

  6 hours

• Change in Leucine turnover

  6 hours

• Change in Isoleucine turnover

  6 hours

• Change in Valine turnover

  6 hours

• Change in plasma lactate concentration

  6 hours

Study Arms (4)

Caseinate protein intake

EXPERIMENTAL

18 mg protein/kg body weight caseinate and 46 mg maltodextrin / kg body weight per 20 min sip feeding

Dietary Supplement: Caseinate

Whey protein isolate intake

EXPERIMENTAL

18 mg protein/kg body weight whey protein isolate and 46 mg maltodextrin / kg body weight per 20 min sip feeding

Dietary Supplement: Whey protein isolate

Soy protein intake

EXPERIMENTAL

18 mg protein/kg body weight soy and 46 mg maltodextrin / kg body weight per 20 min sip feeding

Dietary Supplement: Soy

soy+BCAA protein intake

EXPERIMENTAL

18 mg protein/kg body weight soy+BCAA and 46 mg maltodextrin / kg body weight per 20 min sip feeding

Dietary Supplement: soy+BCAA

Interventions

Caseinate DIETARY_SUPPLEMENT

18 mg protein/kg body weight caseinate and 46 mg maltodextrin / kg body weight per 20 min sip feeding

Also known as: casein

Caseinate protein intake

Whey protein isolate DIETARY_SUPPLEMENT

18 mg protein/kg body weight whey protein isolate and 46 mg maltodextrin / kg body weight per 20 min sip feeding

Also known as: Whey

Whey protein isolate intake

Soy DIETARY_SUPPLEMENT

18 mg protein/kg body weight soy and 46 mg maltodextrin / kg body weight per 20 min sip feeding

Soy protein intake

soy+BCAA DIETARY_SUPPLEMENT

18 mg protein/kg body weight soy+BCAA and 46 mg maltodextrin / kg body weight per 20 min sip feeding

soy+BCAA protein intake

Eligibility Criteria

• Age: 45 Years+
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Irreversible chronic airflow limitation (FEV1 \<70% of predicted)
• Clinically stable condition

You may not qualify if:

• Oxygen supplementation
• Respiratory tract infection or exacerbation of his disease at least 4 weeks prior to the study
• Oral corticosteroids as maintenance medication
• Other concomitant metabolic disease (ie malignancy, cardiac failure, recent surgery, severe endocrine, hepatic or renal disorder)

Sponsors & Collaborators

• Maastricht University Medical Center: lead
• European Dairy Association (EDA), Brussels: collaborator

Study Sites (1)

Maastricht UMC

Maastricht, Netherlands

Location

Related Publications (4)

• Engelen MP, Rutten EP, De Castro CL, Wouters EF, Schols AM, Deutz NE. Supplementation of soy protein with branched-chain amino acids alters protein metabolism in healthy elderly and even more in patients with chronic obstructive pulmonary disease. Am J Clin Nutr. 2007 Feb;85(2):431-9. doi: 10.1093/ajcn/85.2.431.

  PMID: 17284740 RESULT

• Engelen MP, Rutten EP, De Castro CL, Wouters EF, Schols AM, Deutz NE. Altered interorgan response to feeding in patients with chronic obstructive pulmonary disease. Am J Clin Nutr. 2005 Aug;82(2):366-72. doi: 10.1093/ajcn.82.2.366.

  PMID: 16087980 RESULT

• Engelen MP, De Castro CL, Rutten EP, Wouters EF, Schols AM, Deutz NE. Enhanced anabolic response to milk protein sip feeding in elderly subjects with COPD is associated with a reduced splanchnic extraction of multiple amino acids. Clin Nutr. 2012 Oct;31(5):616-24. doi: 10.1016/j.clnu.2012.04.006. Epub 2012 Jun 6.

  PMID: 22682082 DERIVED

• Engelen MP, Rutten EP, De Castro CL, Wouters EF, Schols AM, Deutz NE. Casein protein results in higher prandial and exercise induced whole body protein anabolism than whey protein in chronic obstructive pulmonary disease. Metabolism. 2012 Sep;61(9):1289-300. doi: 10.1016/j.metabol.2012.03.001. Epub 2012 Apr 17.

  PMID: 22512824 DERIVED

MeSH Terms

Conditions

Pulmonary Disease, Chronic Obstructive; Motor Activity

Interventions

Caseins; Whey

Condition Hierarchy (Ancestors)

Lung Diseases, Obstructive; Lung Diseases; Respiratory Tract Diseases; Chronic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Behavior

Intervention Hierarchy (Ancestors)

Milk Proteins; Animal Proteins, Dietary; Dietary Proteins; Proteins; Amino Acids, Peptides, and Proteins; Phosphoproteins; Milk; Beverages; Diet, Food, and Nutrition; Physiological Phenomena; Dairy Products; Food; Food and Beverages

Study Officials

• Nicolaas EP Deutz, MD, PhD

  University of Arkansas

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: August 9, 2011
• First Posted: August 17, 2011
• Study Start: December 1, 2002
• Primary Completion: December 1, 2003
• Study Completion: December 1, 2004
• Last Updated: August 17, 2011

Record last verified: 2011-08

Locations

NL (1)