NCT01399996

Brief Summary

In the proposed work we will evaluate the efficacy of a probiotic bacterium (Bifidobacterium animalis subsp. lactis BB12) delivered in a yogurt smoothie (organism to be added before or after fermentation) or as a supplement (tablet) by assessing 1) bowel habits (transit time); 2) the ecology of the bacterial community in the GIT; and 3) immune status of healthy human volunteers. In addition to providing information about the relative efficacy of the delivery vehicles on probiotic function it will provide novel information about the influence of the yogurt smoothie alone (control) on all the parameters measured.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
36

participants targeted

Target at P50-P75 for phase_1

Timeline
Completed

Started Apr 2012

Typical duration for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

July 19, 2011

Completed
3 days until next milestone

First Posted

Study publicly available on registry

July 22, 2011

Completed
8 months until next milestone

Study Start

First participant enrolled

April 1, 2012

Completed
2.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 1, 2014

Completed
1 month until next milestone

Study Completion

Last participant's last visit for all outcomes

August 1, 2014

Completed
Last Updated

August 21, 2023

Status Verified

August 1, 2023

Enrollment Period

2.2 years

First QC Date

July 19, 2011

Last Update Submit

August 16, 2023

Conditions

Keywords

probioticmicrobiotatransit timeimmunityimmune functionconstipationbowel function

Outcome Measures

Primary Outcomes (1)

  • Gastrointestinal transit time

    Gastrointestinal transit time is the amount of time it takes for food to travel through the digestive tract to be excreted. It will be measured using the SmartPill wireless motility capsule.

    Weeks 4, 10, 16, 22

Secondary Outcomes (2)

  • Change from baseline in the fecal microbiota profile at 4 weeks following each of the 4 interventions and 1 free living period

    Weeks 4, 10, 16, 22 and 28

  • Change from baseline in immune status at 4 weeks following each of the 4 interventions and 1 free living period

    Weeks 4, 10, 16, 22, 28

Study Arms (4)

Yogurt smoothie without probiotic.

PLACEBO COMPARATOR
Dietary Supplement: Yogurt smoothie without probiotic

Probiotic added post fermentation.

EXPERIMENTAL
Dietary Supplement: Yogurt smoothie with probiotic added post fermentation

Probiotic added pre-fermentation.

EXPERIMENTAL
Dietary Supplement: Yogurt smoothie with probiotic added pre-fermentation

A capsule containing the probiotic.

EXPERIMENTAL
Dietary Supplement: A capsule containing the probiotic

Interventions

A daily 8 oz (240 mg) serving will provide 10x\^y5 cfu/ml of the probiotic (Bifidobacterium animalis subsp. lactis BB12).

Also known as: Yogurt
Yogurt smoothie without probiotic.

A daily 8 oz (240 mg) serving will provide 10x\^y5 cfu/ml of the probiotic (Bifidobacterium animalis subsp. lactis BB12).

Also known as: Yogurt with probiotic
Probiotic added post fermentation.

A daily 8 oz (240 mg) serving will provide 10x\^y5 cfu/ml of the probiotic (Bifidobacterium animalis subsp. lactis BB12).

Also known as: Yogurt with probiotic
Probiotic added pre-fermentation.

A capsule taken daily will provide between 10x\^y9 and 10x\^y10 cfu/ml of the probiotic (Bifidobacterium animalis subsp. lactis BB12).

Also known as: Probiotic capsule
A capsule containing the probiotic.

Eligibility Criteria

Age18 Years - 40 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Generally healthy
  • Men and women
  • years of age
  • Body mass index between 20 and 35 kg/m\^2
  • Persons with irregular bowel function as determined using the Rome III criteria (1) which is used to classify functional gastrointestinal disorders.
  • Increased gastrointestinal transit time \> 60 hours.

You may not qualify if:

  • Smoking and/or use of other tobacco products
  • Blood pressure greater than 140/90 mm Hg
  • A history of myocardial infarction, stroke, diabetes mellitus, liver disease, kidney disease and thyroid disease (unless controlled by medication and blood results within the previous 6 months are provided).
  • Lactation, pregnancy or desire to become pregnant during the study
  • Use of cholesterol-lowering medication
  • Refusal to discontinue intake of putative cholesterol-lowering supplements (psyllium, fish oil capsules, soy lecithin, niacin, fiber, flax, phytoestrogens, stanol/sterol supplemented foods
  • Refusal to discontinue probiotics,nutritional supplements, herbs or vitamins
  • Vegetarianism/Veganism
  • Lactose intolerance
  • Clinical diagnosis of Inflammatory Bowel Disease (IBD), e.g. Crohn's Disease or ulcerative colitis
  • Excessive alcohol consumption (\> 14 standard drinks per week)
  • Chronic use of anti-inflammatory medications (unless able to discontinue)
  • Individuals taking stool softeners or enemas on a regular basis.
  • Allergy to polyvinyl chloride (PVC) or any other type of plastic
  • Individuals with a bowel transit time of \< 60 hours at time of screening
  • +5 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Penn State University

University Park, Pennsylvania, 16802, United States

Location

Related Publications (4)

  • Drossman DA, Dumitrascu DL. Rome III: New standard for functional gastrointestinal disorders. J Gastrointestin Liver Dis. 2006 Sep;15(3):237-41.

    PMID: 17013448BACKGROUND
  • Ba Z, Lee Y, Meng H, Kris-Etherton PM, Rogers CJ, Lewis ZT, Mills DA, Furumoto EJ, Rolon ML, Fleming JA, Roberts RF. Matrix Effects on the Delivery Efficacy of Bifidobacterium animalis subsp. lactis BB-12 on Fecal Microbiota, Gut Transit Time, and Short-Chain Fatty Acids in Healthy Young Adults. mSphere. 2021 Aug 25;6(4):e0008421. doi: 10.1128/mSphere.00084-21. Epub 2021 Jul 7.

  • Lee Y, Ba Z, Roberts RF, Rogers CJ, Fleming JA, Meng H, Furumoto EJ, Kris-Etherton PM. Effects of Bifidobacterium animalis subsp. lactis BB-12(R) on the lipid/lipoprotein profile and short chain fatty acids in healthy young adults: a randomized controlled trial. Nutr J. 2017 Jun 29;16(1):39. doi: 10.1186/s12937-017-0261-6.

  • Meng H, Lee Y, Ba Z, Peng J, Lin J, Boyer AS, Fleming JA, Furumoto EJ, Roberts RF, Kris-Etherton PM, Rogers CJ. Consumption of Bifidobacterium animalis subsp. lactis BB-12 impacts upper respiratory tract infection and the function of NK and T cells in healthy adults. Mol Nutr Food Res. 2016 May;60(5):1161-71. doi: 10.1002/mnfr.201500665. Epub 2016 Mar 1.

MeSH Terms

Conditions

Constipation

Interventions

ProbioticsYogurt

Condition Hierarchy (Ancestors)

Signs and Symptoms, DigestiveSigns and SymptomsPathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Dietary SupplementsFoodDiet, Food, and NutritionPhysiological PhenomenaFood and BeveragesCultured Milk ProductsMilkBeveragesFermented FoodsDairy Products

Study Officials

  • Penny M Kris-Etherton, PhD

    Penn State University

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
BASIC SCIENCE
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 19, 2011

First Posted

July 22, 2011

Study Start

April 1, 2012

Primary Completion

July 1, 2014

Study Completion

August 1, 2014

Last Updated

August 21, 2023

Record last verified: 2023-08

Locations