NCT01391494

Brief Summary

Enterovirus 71 (EV71), a major pathogen that is responsible for causing hand-foot-and-mouth disease (HFMD) worldwide, is a member of the Human Enterovirus species A, family Picornaviridae. Since the late 1990s, a series of large HFMD epidemics caused by EV71 have been reported in the Asia-Pacific region. Notably, there is evidence that the most severe cases from these epidemic outbreaks are associated with neurological disorders with CNS involvement caused by EV71 infection. Because of these EV71 infection-related public health issues, the research and development of EV71 vaccine candidates have been heavily promoted. Recently, several EV71 vaccine candidates have been evaluated in animals but no final results of clinical trials, including inactivated vaccine, attenuated vaccine, subunit vaccine, DNA vaccine, epitope peptide vaccine, virus-like particles (VLPs). Basing on the previous studies of elicited protection in mice and rhesus monkeys, a formalin-inactivated EV71 vaccine (Human Diploid cell, KMB-17 Cell) has been licensed by SFDA in China, Dec. 2010. The phase I clinical trial has been carried out, during four months, in Guangxi Province, China. The purpose of this study is to evaluate the safety, tolerability and immunogenicity of the formalin-inactivated EV71 vaccine in Chinese adults (from 18 to 49 years old), children (from 3 to 11 years old) and infants (from 6 to 35 months old).

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
288

participants targeted

Target at P75+ for phase_1

Timeline
Completed

Started Feb 2011

Shorter than P25 for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

February 1, 2011

Completed
4 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2011

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2011

Completed
1 month until next milestone

First Submitted

Initial submission to the registry

July 11, 2011

Completed
1 day until next milestone

First Posted

Study publicly available on registry

July 12, 2011

Completed
Last Updated

October 11, 2023

Status Verified

October 1, 2023

Enrollment Period

4 months

First QC Date

July 11, 2011

Last Update Submit

October 7, 2023

Conditions

Keywords

Human Enterovirus 71 (EV71)Hand, Foot and Mouth DiseaseInactivated VaccineHuman Diploid cellSafetyImmunogenicityAdverse reactions associated with vaccine

Outcome Measures

Primary Outcomes (6)

  • Evaluate the Safety of Inactivated EV 71 Vaccine (Human Diploid Cell, KMB-17) in Chinese Adults.

    Adverse reactions associated with vaccine were observed in Chinese Adults (from 18 to 49 years old) after the first vaccination.

    within the first 14 days after the first vaccination

  • Evaluate the Safety of Inactivated EV 71 Vaccine (Human Diploid Cell, KMB-17) in Chinese Adults.

    Adverse reactions associated with vaccine were observed in Chinese Adults (from 18 to 49 years old) after the second vaccination.

    within the first 4 days after the second vaccination

  • Evaluate the Safety of Inactivated EV 71 Vaccine (Human Diploid Cell, KMB-17) in Chinese Children.

    Adverse reactions associated with vaccine were observed in Chinese Children (from 3 to 11 years old) after the first vaccination.

    within the first 14 days after the first vaccination

  • Evaluate the Safety of Inactivated EV 71 Vaccine (Human Diploid Cell, KMB-17) in Chinese Children.

    Adverse reactions associated with vaccine were observed in Chinese Children (from 3 to 11 years old) after the second vaccination.

    within the first 4 days after the second vaccination

  • Evaluate the safety of Inactivated EV 71 Vaccine (Human Diploid Cell, KMB-17) in Chinese Infants.

    Adverse reactions associated with vaccine were observed in Chinese Infants (from 6 to 35 months old) after the first vaccination.

    within the first 28 days after the first vaccination

  • Evaluate the safety of Inactivated EV 71 Vaccine (Human Diploid Cell, KMB-17) in Chinese Infants.

    Adverse reactions associated with vaccine were observed in Chinese Infants (from 6 to 35 months old) after the second vaccination.

    within the first 28 days after the second vaccination

Secondary Outcomes (8)

  • Evaluate the seroconversion rate of anti-EV71 antibodies in serum of adults, children and infant, after first vaccination.

    within the first 14 or 28 days after the first vaccination

  • Evaluate the seroconversion rate of anti-EV71 antibodies in serum of adults, children and infant, after second vaccination.

    within the first 4 or 28 days after the second vaccination

  • Evaluate the seroconversion rate of antinuclear antibodies in serum of adults, children and infant, after first vaccination.

    within the first 14 or 28 days after the first vaccination

  • Evaluate the seroconversion rate of antinuclear antibodies in serum of adults, children and infant, after second vaccination.

    within the first 4 or 28 days after the second vaccination

  • Evaluate the abnormity change of live and kidney function indexes in serum of adults, children and infant, after first vaccination.

    within the first 14 or 28 days after the first vaccination

  • +3 more secondary outcomes

Study Arms (15)

160Eu/0.5ml in adults

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 160Eu/0.5ml in 12 adults aged 18-49 years old on day 0, 14.

Biological: 160Eu/0.5ml in adults

320Eu/0.5ml in adults

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 320Eu/0.5ml in 12 adults aged 18-49 years old on day 0, 14.

Biological: 320Eu/0.5ml in adults

640Eu/0.5ml in adults

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 640Eu/0.5ml in 12 adults aged 18-49 years old on day 0, 14.

Biological: 640Eu/0.5ml in adults

1280Eu/0.5ml (without adjuvant) in adults

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 1280Eu/0.5ml (without adjuvant) in 12 adults aged 18-49 years old on day 0, 14.

Biological: 1280Eu/0.5ml (without adjuvant) in 12 adults

0Eu/0.5ml in adults

PLACEBO COMPARATOR

0Eu/0.5ml placebo in 24 adults aged 18-49 years old on day 0, 14.

Biological: 0Eu/0.5ml in adults

160Eu/0.5ml in children

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 160Eu/0.5ml in 12 children aged 3-11 years old on day 0, 14.

Biological: 160Eu/0.5ml in children

320Eu/0.5ml in children

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 320Eu/0.5ml in 12 children aged 3-11 years old on day 0, 14.

Biological: 320Eu/0.5ml in children

640Eu/0.5ml in children

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 640Eu/0.5ml in 12 children aged 3-11 years old on day 0, 14.

Biological: 640Eu/0.5ml in children

1280Eu/0.5ml (without adjuvant) in children

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 1280Eu/0.5ml (without adjuvant) in 12 children aged 3-11 years old on day 0, 14.

Biological: 1280Eu/0.5ml (without adjuvant) in children

0Eu/0.5ml in children

PLACEBO COMPARATOR

0Eu/0.5ml placebo in 24 children aged 3-11 years old on day 0, 14.

Biological: 0Eu/0.5ml in children

160Eu/0.5ml in infants

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 160Eu/0.5ml in 24 infants aged 6-35 months old on day 0, 28.

Biological: 160Eu/0.5ml in infants

320Eu/0.5ml in infants

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 320Eu/0.5ml in 24 infants aged 6-35 months old on day 0, 28.

Biological: 320Eu/0.5ml in infants

640Eu/0.5ml in infants

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 640Eu/0.5ml in 24 infants aged 6-35 months old on day 0, 28.

Biological: 640Eu/0.5ml in infants

1280Eu/0.5ml (without adjuvant) in infants

EXPERIMENTAL

inactivated EV71 vaccine (KMB-17) of 1280Eu/0.5ml (without adjuvant) in 24 infants aged 6-35 months old on day 0, 28.

Biological: 1280Eu/0.5ml (without adjuvant) in infants

0Eu/0.5ml in infants

PLACEBO COMPARATOR

0Eu/0.5ml placebo in 48 infants aged 6-35 months old on day 0, 28.

Biological: 0Eu/0.5ml in infants

Interventions

inactivated EV71 vaccine (KMB-17) of 160Eu/0.5ml in 12 adults aged 18-49 years old on day 0, 14.

160Eu/0.5ml in adults

inactivated EV71 vaccine (KMB-17) of 320Eu/0.5ml in 12 adults aged 18-49 years old on day 0, 14.

320Eu/0.5ml in adults

inactivated EV71 vaccine (KMB-17) of 640Eu/0.5ml in 12 adults aged 18-49 years old on day 0, 14.

640Eu/0.5ml in adults

inactivated EV71 vaccine (KMB-17) of 1280Eu/0.5ml (without adjuvant) in 12 adults aged 18-49 years old on day 0, 14.

1280Eu/0.5ml (without adjuvant) in adults

0Eu/0.5ml placebo in 24 adults aged 18-49 years old on day 0, 14.

0Eu/0.5ml in adults

inactivated EV71 vaccine (KMB-17) of 160Eu/0.5ml in 12 children aged 3-11 years old on day 0, 14.

160Eu/0.5ml in children

inactivated EV71 vaccine (KMB-17) of 320Eu/0.5ml in 12 children aged 3-11 years old on day 0, 14.

320Eu/0.5ml in children

inactivated EV71 vaccine (KMB-17) of 640Eu/0.5ml in 12 children aged 3-11 years old on day 0, 14.

640Eu/0.5ml in children

inactivated EV71 vaccine (KMB-17) of 1280Eu/0.5ml (without adjuvant) in 12 children aged 3-11 years old on day 0, 14.

1280Eu/0.5ml (without adjuvant) in children

0Eu/0.5ml placebo in 24 children aged 3-11 years old on day 0, 14.

0Eu/0.5ml in children

inactivated EV71 vaccine (KMB-17) of 160Eu/0.5ml in 24 infants aged 6-35 months old on day 0, 28.

160Eu/0.5ml in infants

inactivated EV71 vaccine (KMB-17) of 320Eu/0.5ml in 24 infants aged 6-35 months old on day 0, 28.

320Eu/0.5ml in infants

inactivated EV71 vaccine (KMB-17) of 640Eu/0.5ml in 24 infants aged 6-35 months old on day 0, 28.

640Eu/0.5ml in infants

inactivated EV71 vaccine (KMB-17) of 1280Eu/0.5ml (without adjuvant) in 24 infants aged 6-35 months old on day 0, 28.

1280Eu/0.5ml (without adjuvant) in infants

0Eu/0.5ml placebo in 48 infants aged 6-35 months old on day 0, 28.

0Eu/0.5ml in infants

Eligibility Criteria

Age6 Months - 49 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64)

You may qualify if:

  • Healthy subjects (18-49 years old adults) as established by medical history and clinical examination
  • The subjects oneself or their legal guardian must be aware of this vaccines
  • Voluntarily participate in the study and signed Informed Consent Form
  • Subjects with temperature ≤ 37.0℃
  • With the ability and objective to comply with the requirements of the protocol
  • Persist for a 2-month visit and receive blood tests according to program requirements

You may not qualify if:

  • Subject who has a clinical diagnosis history of Hand, Foot and Mouth Disease (HFMD)
  • Allergy or serious side-effects to a vaccine or any ingredient of vaccine
  • Epilepsy, seizures, convulsions, neurological illness
  • Congenital or hereditary immunodeficiency
  • Autoimmune disease
  • Severe malnutrition or dysgenopathy
  • Asthma, thyroidectomy, angioneurotic edema, diabetes or cancer
  • Asplenia, functional asplenia, and any circumstances leading to the asplenia or splenectomy
  • Clinical diagnosis of coagulopathy (such as clotting factor deficiency, coagulation disorders, platelet abnormalities), significant bruising or blood clotting disorder
  • Acute illness or acute exacerbation of chronic disease in last 7 days
  • Any prior administration of immunodepressant or corticosteroids in last 6 months
  • Any prior administration of blood products in last 3 months
  • Any prior administration of live-attenuated vaccine in last 28 days or 1 months
  • Any prior administration of subunit or inactivated vaccines in last 14 days
  • Under the anti-TB prevention or therapy
  • +63 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Guangxi Provincial Center for Diseases Control and Prevention

Nanning, Guangxi, 530028, China

Location

Related Publications (1)

  • Liu L, Zhang Y, Wang J, Zhao H, Jiang L, Che Y, Shi H, Li R, Mo Z, Huang T, Liang Z, Mao Q, Wang L, Dong C, Liao Y, Guo L, Yang E, Pu J, Yue L, Zhou Z, Li Q. Study of the integrated immune response induced by an inactivated EV71 vaccine. PLoS One. 2013;8(1):e54451. doi: 10.1371/journal.pone.0054451. Epub 2013 Jan 23.

Related Links

MeSH Terms

Conditions

Hand, Foot and Mouth Disease

Condition Hierarchy (Ancestors)

Coxsackievirus InfectionsEnterovirus InfectionsPicornaviridae InfectionsRNA Virus InfectionsVirus DiseasesInfections

Study Officials

  • Zhaojun Mo, Master

    Guangxi Provincial Center for Diseases Control and Prevention

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 11, 2011

First Posted

July 12, 2011

Study Start

February 1, 2011

Primary Completion

June 1, 2011

Study Completion

June 1, 2011

Last Updated

October 11, 2023

Record last verified: 2023-10

Locations