Effect of Broccoli Sprout on Blood Levels of Sulforaphane to Reduce Responsiveness of Immune System

NCT01357070 | Completed DMC

• 1 other identifier: 2-bao
• Study Type: interventional
• Target: 6
• Locations: 1 country
• Sites: 1

Brief Summary

The investigators suggest that inducing anti-oxidant enzymes indirectly may be an effective means of providing vascular protection. Sulforaphane, a naturally occurring compound found in green vegetables (including broccoli, brussel sprouts and cauliflower) is able to protect against cell inflammatory stress by inducing a number of anti-oxidant molecules. Targeted studies on the consumption of broccoli and related vegetables have been shown to be associated with reduced risk of coronary artery disease. In the present study the investigators want to test whether the consumption of a "broccoli smoothie" containing sulforaphane can protect white blood cells from becoming activated in the presence of an experimental stress and how long this protective effect lasts for. To do this, the investigators will be analysing inflammatory changes in blood samples taken at different times during the study.

Conditions

Healthy Volunteers

Keywords

white blood cell; broccoli; P38 Map Kinase

Outcome Measures

Primary Outcomes (1)

• Resistance of leucocytes to inflammatory activation following an experimental stress

  Participants will consume a brocco-sprout homogenate. Blood will be sampled for intracellular markers of leukocyte stress after ex-vivo stimulus with Phorbol ester / Tumour Necrosis Factor alpha. The following parameters will be recorded. 1. Reactive oxygen species detection using aminophenylfluoresceine dye 2. p38 MAP kinase induction 3. NF-kB induction

  24 hours after consumption of the third homogenate

Study Arms (2)

Brocco-sprout homogenate

ACTIVE COMPARATOR

Dietary Supplement: Brocco-sprout homogenate

Alfalfa sprout homogenate

SHAM COMPARATOR

Dietary Supplement: Alfalfa sprout homogenate

Interventions

Brocco-sprout homogenate DIETARY_SUPPLEMENT

Participants will be asked to orally consume on three consecutive days a brocco-sprout homogenate (70g dry weight).

Brocco-sprout homogenate

Alfalfa sprout homogenate DIETARY_SUPPLEMENT

Participants will be asked to orally consume on three consecutive days an alfalfa sprout homogenate.

Alfalfa sprout homogenate

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Age 18+ years
• Able to commit for the two week duration of the trial

You may not qualify if:

• Age \<18 years
• Pregnancy
• Vegetarian
• History of allergy
• Current smoker or smoking cessation within the last 3 months
• Current use of inhaled, topical or systemic corticosteroids or within the last 2 weeks
• Current use of non-steroidal anti-inflammatory use or within the last 1 week
• Current use of nutritional or multivitamin supplements
• Current participation in any other Randomised controlled trial

Sponsors & Collaborators

• Imperial College London: lead

Study Sites (1)

Cardiovascular Medicine Research Unit, Hammersmith Hospital

London, W12 0NN, United Kingdom

Location

Study Officials

• Paul C Evans, BSc MSc PhD

  Imperial College London

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: May 17, 2011
• First Posted: May 20, 2011
• Study Start: May 1, 2011
• Primary Completion: January 1, 2014
• Study Completion: January 1, 2014
• Last Updated: July 8, 2019

Record last verified: 2019-07

Data Sharing

• IPD Sharing: Will not share

Locations

GB (1)