Bronchoalveolar Immune Response Determination

NCT01237145 | Completed

• 1 other identifier: BIRD01
• Study Type: observational
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to establish if cells present in lung tissue during extrinsic allergic alveolitis (EAA) are able to elicit an immune response against specific antigens. The hypothesis is: cells in BAL lavage fluid from patients with EAA will be responsive to specific antigens and this response can be measured using ELISPOT.

Conditions

Extrinsic Allergic Alveolitis

Keywords

pigeon; bird fancier; extrinsic allergic alveolitis; BAL; broncho-alveolar lavage; elispot; antigen

Outcome Measures

Primary Outcomes (1)

• ELISPOT response in cells obtained from broncho-alveolar lavage

  All elispots will be performed the same day, the end point will be available the next day

  2 days

Secondary Outcomes (1)

• Elispot response in cells from blood

  2 days

Study Arms (2)

non-EAA, pigeon

subject with BAL because of diseases not suspected to be EAA

EAA, pigeon

Bird fanciers with a typical clinical presentation suspected for pigeon induced EAA

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients suspected to have pigeon induced extrinsic allergic alveolitis will be recruited by advertisment in bird fanciers journals. Controls will be patients at the department of pulmonology and tuberculosis needing examination for other reasons

You may qualify if:

• suspicion of pigeon induced extrinsic alveolitis

You may not qualify if:

• use of oral steroids
• unable to give informed consent
• age below 18

Sponsors & Collaborators

• Bossink, A.W.J.: lead

Study Sites (1)

Diakonessenhuis Utrecht

Utrecht, 3582 KE, Netherlands

Location

Related Publications (3)

• Jafari C, Thijsen S, Sotgiu G, Goletti D, Dominguez Benitez JA, Losi M, Eberhardt R, Kirsten D, Kalsdorf B, Bossink A, Latorre I, Migliori GB, Strassburg A, Winteroll S, Greinert U, Richeldi L, Ernst M, Lange C; Tuberculosis Network European Trialsgroup. Bronchoalveolar lavage enzyme-linked immunospot for a rapid diagnosis of tuberculosis: a Tuberculosis Network European Trialsgroup study. Am J Respir Crit Care Med. 2009 Oct 1;180(7):666-73. doi: 10.1164/rccm.200904-0557OC. Epub 2009 Jul 9.

  PMID: 19590020 BACKGROUND

• Losi M, Bossink A, Codecasa L, Jafari C, Ernst M, Thijsen S, Cirillo D, Ferrarese M, Greinert U, Fabbri LM, Richeldi L, Lange C; European Tuberculosis Network TBNET. Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy. Eur Respir J. 2007 Dec;30(6):1173-9. doi: 10.1183/09031936.00067307. Epub 2007 Aug 22.

  PMID: 17715165 BACKGROUND

• Kosters K, Nau R, Bossink A, Greiffendorf I, Jentsch M, Ernst M, Thijsen S, Hinks T, Lalvani A, Lange C. Rapid diagnosis of CNS tuberculosis by a T-cell interferon-gamma release assay on cerebrospinal fluid mononuclear cells. Infection. 2008 Dec;36(6):597-600. doi: 10.1007/s15010-007-7316-0. Epub 2008 Jan 12.

  PMID: 18193383 BACKGROUND

Biospecimen

Retention: SAMPLES WITHOUT DNA

Cells obtained from Broncho alveolar lavage and blood collection

MeSH Terms

Conditions

Alveolitis, Extrinsic Allergic

Condition Hierarchy (Ancestors)

Lung Diseases, Interstitial; Lung Diseases; Respiratory Tract Diseases; Respiratory Hypersensitivity; Hypersensitivity, Immediate; Hypersensitivity; Immune System Diseases

Study Officials

• Aik Bossink, MD PhD

  Diakonessenhuis, Utrecht

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: INDIV
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Dr

Study Record Dates

• First Submitted: November 7, 2010
• First Posted: November 9, 2010
• Study Start: September 1, 2011
• Primary Completion: September 1, 2012
• Study Completion: December 1, 2012
• Last Updated: January 19, 2018

Record last verified: 2018-01

Locations

NL (1)