Cardiomyocyte Apoptosis Following Antegrade and Retrograde Cardioplegia

NCT01185912 | Completed

• 1 other identifier: 1/2006
• Study Type: observational
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Retrograde cardioplegia during heart surgery is associated with inadequate myocardial protection. In experimental animal study retrograde cardioplegia induces more cardiomyocyte apoptosis when compared to antegrade cardioplegia. 20 volunteer patients under going elective aortic valve surgery will be divided in to two groups: 10 patients receiving only antegrade cardioplegia and 10 patients receiving only retrograde cardioplegia. Pre- and post-operatively cardiac MRI and cardiac ultrasound will be performed. During the surgery ventricular muscle samples will be taken in order to assess cardiomyocyte apoptosis. Aims of the study: 1. whether the cardiomyocyte apoptosis is significantly more induced after retrograde cardioplegia than antegrade cardioplegia, 2. whether the amount of apoptotic cardiomyocytes correlates to conventional markers of myocardial injury after cardiac surgery, 3. whether the extent of irreversible myocardial damage by apoptosis correlates to post-ischemic contractile dysfunction after cardiac surgery, as measured with echocardiography, 4. whether the amount of cardiomyocyte apoptosis correlates to long term outcome and cardiac function as measured with MRI.

Conditions

Cardiomyocyte Apoptosis

Keywords

Cardiac MRI; Cardiac ultrasound; Cardiac surgery

Study Arms (1)

Cardiomyocyte apoptosis

Elective aortic valve replacement patience

Procedure: Ventricle muscle tissue samples

Interventions

Ventricle muscle tissue samples PROCEDURE

Cardiomyocyte apoptosis

Eligibility Criteria

• Age: 60 Years - 90 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

20 patients (male and female) undergoing elective aortic valve replacement surgery due to aortic valve stenosis or combined aortic valve disease in Turku University Hospital, Department of Surgery.

You may qualify if:

• aortic valve stenosis or combined aortic valve disease

You may not qualify if:

• angiographically verified hemodynamically significant coronary artery disease impaired left ventricular function (left ventricular ejection fraction \<50%) surgery including additional procedures other than aortic valve replacement cancer

Sponsors & Collaborators

• Turku University Hospital: lead
• Finnish Foundation for Cardiovascular Research: collaborator

Study Sites (1)

Turku University Hospital, Department of Surgery

Turku, 20521, Finland

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Transmyocardial needle biopsies

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER GOV
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: MD

Study Record Dates

• First Submitted: June 24, 2010
• First Posted: August 20, 2010
• Study Start: January 1, 2008
• Primary Completion: January 1, 2010
• Study Completion: January 1, 2010
• Last Updated: May 20, 2015

Record last verified: 2015-05

Locations

FI (1)