Genetics of the Early and Late Response to Allergen Challenge

NCT01113697 | Completed

• 1 other identifier: H09-02114
• Study Type: observational
• Target: 520
• Locations: 1 country
• Sites: 5

Brief Summary

The investigators are investigating the early and late responses to allergen challenge. The research participants who the investigators will study (from three cohorts) will be part of independently-approved studies involving allergen challenge. Due to the uniqueness of the cohorts for novel genetic study, it is logical that the investigators should initially undertake hypothesis-generating experiments. The investigators will obtain blood samples from the participants, both pre-challenge and post-challenge. The investigators will determine gene expression and protein differences between these samples, and investigate if there are inherited genetic differences between individuals that may predict their specific responses to allergens.

Conditions

Asthma; Allergic Rhinitis

Keywords

allergen challenge; blood draw; gene expression analysis; protein analysis; genetics

Outcome Measures

Primary Outcomes (1)

• Observational trial: Early versus Dual Asthmatic Response

  8 hours post allergen challenge

Study Arms (3)

Clinical Investigator Collaborative (CIC) Asthma study cohort

Participants will be healthy volunteer research subjects with allergic asthma who will have an allergen inhalation challenge at CIC sites across Canada.

Western Red Cedar Asthma study cohort

Participants will be volunteer research subjects with Western Red Cedar asthma, who will have a plicatic acid inhalation challenge at The Lung Centre at Vancouver General Hospital.

Environmental Exposure Unit (EEU), Kingston General Hospital

Subjects will be over 19 years of age so that they qualify for studies involving allergen challenge.

Eligibility Criteria

• Age: 19 Years - 55 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

Asthmatic and/or allergic rhinitis ('hay fever') individuals, 19 years or older

You may qualify if:

• participants will be healthy volunteer research subjects with allergic asthma, or
• participants will be volunteer research subjects with Western Red Cedar asthma, or
• participants will be healthy volunteer research subjects with allergic rhinitis (hay fever)
• all participants will be over 19 years of age so that they qualify for studies involving allergen challenge

Sponsors & Collaborators

• University of British Columbia: lead
• Allergy, Genes and Environment Network (AllerGen): collaborator
• Networks of Centres of Excellence of Canada: collaborator

Study Sites (5)

University of British Columbia

Vancouver, British Columbia, Canada

Location

McMaster University

Hamilton, Ontario, Canada

Location

Queen's University

Kingston, Ontario, Canada

Location

University of Saskatchewan

Saskatoon, Saskatchewan, Canada

Location

Laval University

Québec, Canada

Location

Related Publications (4)

• Kam SH, Singh A, He JQ, Ruan J, Gauvreau GM, O'Byrne PM, Fitzgerald JM, Tebbutt SJ. Peripheral blood gene expression changes during allergen inhalation challenge in atopic asthmatic individuals. J Asthma. 2012 Apr;49(3):219-26. doi: 10.3109/02770903.2011.654300. Epub 2012 Feb 9.

  PMID: 22316092 RESULT

• Singh A, Yamamoto M, Kam SH, Ruan J, Gauvreau GM, O'Byrne PM, FitzGerald JM, Schellenberg R, Boulet LP, Wojewodka G, Kanagaratham C, De Sanctis JB, Radzioch D, Tebbutt SJ. Gene-metabolite expression in blood can discriminate allergen-induced isolated early from dual asthmatic responses. PLoS One. 2013 Jul 2;8(7):e67907. doi: 10.1371/journal.pone.0067907. Print 2013.

  PMID: 23844124 RESULT

• Yang CX, Singh A, Kim YW, Conway EM, Carlsten C, Tebbutt SJ. Diagnosis of Western Red Cedar Asthma Using a Blood-based Gene Expression Biomarker Panel. Am J Respir Crit Care Med. 2017 Dec 15;196(12):1615-1617. doi: 10.1164/rccm.201608-1740LE. No abstract available.

  PMID: 28463537 RESULT

• Singh A, Shannon CP, Kim YW, Yang CX, Balshaw R, Cohen Freue GV, Gauvreau GM, FitzGerald JM, Boulet LP, O'Byrne PM, Tebbutt SJ. Novel Blood-based Transcriptional Biomarker Panels Predict the Late-Phase Asthmatic Response. Am J Respir Crit Care Med. 2018 Feb 15;197(4):450-462. doi: 10.1164/rccm.201701-0110OC.

  PMID: 29087730 RESULT

Biospecimen

Retention: SAMPLES WITH DNA

Whole blood, plasma, white cells

MeSH Terms

Conditions

Asthma; Rhinitis, Allergic

Condition Hierarchy (Ancestors)

Bronchial Diseases; Respiratory Tract Diseases; Lung Diseases, Obstructive; Lung Diseases; Respiratory Hypersensitivity; Hypersensitivity, Immediate; Hypersensitivity; Immune System Diseases; Rhinitis; Nose Diseases; Otorhinolaryngologic Diseases

Study Officials

• Scott J. Tebbutt, Ph.D

  University of British Columbia

  PRINCIPAL INVESTIGATOR

• Gail M. Bauvreau, Ph.D

  McMaster University

  STUDY DIRECTOR

• Anne K. Ellis, MD

  Queen's University

  STUDY DIRECTOR

• Christopher R. Carlsten, MD

  University of British Columbia

  STUDY DIRECTOR

• Danuta Radzioch, Ph.D

  McGill University

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: April 28, 2010
• First Posted: April 30, 2010
• Study Start: August 1, 2009
• Primary Completion: December 1, 2023
• Study Completion: December 1, 2023
• Last Updated: December 6, 2023

Record last verified: 2023-12

Locations

CA (5)