NCT01044095

Brief Summary

This will be a randomized, open-label, pilot feasibility study of four 2-dose vaccine regimens in healthy volunteers using two commercially available seasonal influenza vaccines to compare immune responses and in vitro cross-reactivity against H5N1. Vaccine doses will be spaced by approximately 8 weeks to allow for optimal prime boost conditions. Humoral, cellular and secretory immune responses will be measured 2 and 4 weeks after each vaccine dose and compared with baseline values.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
26

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Nov 2009

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2009

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

January 6, 2010

Completed
1 day until next milestone

First Posted

Study publicly available on registry

January 7, 2010

Completed
6 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 1, 2010

Completed
1.9 years until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2012

Completed
Last Updated

July 23, 2013

Status Verified

July 1, 2013

Enrollment Period

8 months

First QC Date

January 6, 2010

Last Update Submit

July 22, 2013

Conditions

Keywords

seasonal influenza vaccineavian influenza H5N1cross reactivityprime boost

Outcome Measures

Primary Outcomes (1)

  • antibody titers

    12 weeks

Secondary Outcomes (1)

  • safety of 2 doses of seasonal influenza vaccine in healthy adults

    12 weeks

Study Arms (4)

Autologous prime boost regimen 1

ACTIVE COMPARATOR

FluMist® live intranasal vaccine (LAIV) 0.2mL (0.1mL per nostril): 2 doses separated by 8 weeks (+/- 7 days)

Biological: FluMist® live, attenuated, intranasal seasonal influenza vaccine

Autologous prime boost regimen 2

ACTIVE COMPARATOR

Fluzone® inactivated seasonal influenza virus vaccine intramuscularly: 2 doses separated by 8 weeks (+7 days)

Biological: Fluzone® inactivated influenza virus vaccine

Heterologous prime boost regimen 1

EXPERIMENTAL

FluMist® live, intranasal vaccine single dose, followed by Fluzone® inactivated influenza virus vaccine 8 weeks (+/-7 days) later

Biological: FluMist® live, attenuated, intranasal seasonal influenza vaccineBiological: Fluzone® inactivated influenza virus vaccine

Heterologous prime boost regimen 2

EXPERIMENTAL

Fluzone® inactivated seasonal influenza virus vaccine single dose, followed by FluMist® live, intranasal seasonal influenza vaccine 0.2mL 8 weeks (+/- 7 days) later

Biological: FluMist® live, attenuated, intranasal seasonal influenza vaccineBiological: Fluzone® inactivated influenza virus vaccine

Interventions

FluMist® live, attenuated, intranasal seasonal influenza vaccine 0.2mL (0.1mL per nostril)

Autologous prime boost regimen 1Heterologous prime boost regimen 1Heterologous prime boost regimen 2

Fluzone® inactivated influenza virus vaccine 0.5mL intramuscularly

Autologous prime boost regimen 2Heterologous prime boost regimen 1Heterologous prime boost regimen 2

Eligibility Criteria

Age18 Years - 49 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Aged 18-49 years.
  • Healthy as determined by the PI or designate
  • Willing to give informed consent.
  • Willing to attend follow-up appointments and undergo study procedures.
  • US passport holder residing in Bangkok
  • If military, have a letter of approval for participation from the chain of command (Unit Commander).

You may not qualify if:

  • History of hypersensitivity to eggs, egg proteins, gentamicin, gelatin or arginine.
  • Severe or life-threatening reaction to any previous vaccine.
  • History of chronic respiratory illness, including asthma and sinusitis.
  • History of heart disease
  • History of kidney disease
  • Metabolic disease such as diabetes
  • Immunocompromised as determined by the PI, or a household contact of an immunocompromised individual.
  • History of Guillain-Barre syndrome.
  • Blood disease including sickle cell disease and/or bleeding tendency (by history or based on medical records).
  • Any influenza vaccination within the previous 6 months, or any other vaccine within 30 days of either dose of influenza vaccine.
  • Pregnant woman or nursing mother or unwilling to use reliable contraception during the study period until the final day of follow-up.
  • Blood donation within the preceding 3 months, or screening hemoglobin value of \<12.5 g/dl.
  • Receipt of blood products including immunoglobulins within the preceding 3 months.
  • Receiving any of the following therapies: aspirin, warfarin, theophylline, phenytoin, aminopyrine, immunosuppressive drugs,
  • Receipt of any antiviral agents within 48 hours of vaccine administration.
  • +1 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

United States Embassy Medical Unit

Bangkok, Thailand

Location

Related Publications (1)

  • Bethell D, Saunders D, Jongkaewwattana A, Kramyu J, Thitithayanont A, Wiboon-ut S, Yongvanitchit K, Limsalakpetch A, Kum-Arb U, Uthaimongkol N, Garcia JM, Timmermans AE, Peiris M, Thomas S, Engering A, Jarman RG, Mongkolsirichaikul D, Mason C, Khemnu N, Tyner SD, Fukuda MM, Walsh DS, Pichyangkul S. Evaluation of in vitro cross-reactivity to avian H5N1 and pandemic H1N1 2009 influenza following prime boost regimens of seasonal influenza vaccination in healthy human subjects: a randomised trial. PLoS One. 2013;8(3):e59674. doi: 10.1371/journal.pone.0059674. Epub 2013 Mar 26.

Related Links

MeSH Terms

Conditions

Influenza, Human

Condition Hierarchy (Ancestors)

Respiratory Tract InfectionsInfectionsOrthomyxoviridae InfectionsRNA Virus InfectionsVirus DiseasesRespiratory Tract Diseases

Study Officials

  • Delia B Bethell, BM BCh

    Armed Forces Research Institute of Medical Sciences, Thailand

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
NONE
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Sponsor Type
OTHER GOV
Responsible Party
SPONSOR

Study Record Dates

First Submitted

January 6, 2010

First Posted

January 7, 2010

Study Start

November 1, 2009

Primary Completion

July 1, 2010

Study Completion

June 1, 2012

Last Updated

July 23, 2013

Record last verified: 2013-07

Locations