Effects of Parenteral L-carnitine Supplementation in Premature Neonates

NCT00841295 | Completed DMC

• 4 other identifiers: PHRI06-FL / CARNIPREMAN° EudraCT: 2007-002446-37Réf.CPP: 2007-R24Réf.Afssaps: A70583-46
• Study Type: interventional
• Target: 53
• Locations: 1 country
• Sites: 2

Brief Summary

Background: Carnitine is the essential cofactor for various enzyme activities of human metabolism, especially for the mitochondrial carnitine shuttle that transfers long-chain fatty acids as acylcarnitine esters across the inner mitochondrial membrane for Beta-oxidation and energy production. Intracellular carnitine deficiency induces an impairment of long-chain fatty acid oxidation. In human, approximately 75% of carnitine comes from the diet and 25% from endogenous liver synthesis. In the neonatal period, more specifically in the premature, liver synthesis capacity is reduced because of immaturity of the biosynthetic pathway, and carnitine levels are related to exogenous sources. Traditionally, carnitine is not added to parenteral nutrition. Indeed, without enteral feeds and carnitine supplementation of parenteral nutrition, preterm infants' plasma carnitine levels fall during the first weeks of life, particularly in subjects requiring a prolonged exclusive parenteral nutrition. The potential deleterious role of carnitine deficiency has not been clearly demonstrated in these infants. However, most patients with primary carnitine deficiency, a genetic defect of carnitine transport inducing a severe carnitine deficiency, commonly develop liver symptoms (encompassing visceral steatosis, hyperammonemia and recurrent hypoketotic hypoglycemias) and/or cardiomyopathy and myopathy. In these latter patients, carnitine supplementation improves all the symptoms. Hypothesis: Carnitine deficiency of the premature and very low birth weight infants may be one of the factors involved in the liver disease frequently associated with prolonged parenteral nutrition, and may have deleterious effects on cardiac and muscle metabolism and functions. Aims: To demonstrate beneficial effects of parenteral carnitine supplementation in premature neonates for liver, heart and muscle metabolism and functions. Study Type: Multicentric prospective and randomised study Subjects: Premature and very low birth weight neonates, defined by gestational age minor or equal to 28 weeks and/or birth weight minor or equal to 1000 grams, 80 subjects will be enrolled during 2.5 years Interventions: Arm 1 (experimental): parenteral carnitine supplementation (9 ± 1 mg/kg/d), from day 4, until than enteral nutrition provides sufficient carnitine source; Arm 2 (Placebo comparator): parenteral supplementation with an equivalent volume of sterile water.

Conditions

Complication of Prematurity

Outcome Measures

Primary Outcomes (1)

• Plasma Gamma Glutamyl Transferase level

  After 21 days of parenteral supplementation.

Secondary Outcomes (6)

• Liver function: levels of ammonemia, hyaluronic acid, bilirubin, prothrombin time test, use of ursodeoxycholic acid therapy.

  Short- (during parenteral supplementation) and long- (3 to 5 months of age) term outcome

• Cardiac function: echocardiography, EKG.

  Short- (during parenteral supplementation) and long- (3 to 5 months of age) term outcome

• Muscle integrity: CK levels.

  Short- (during parenteral supplementation) and long- (3 to 5 months of age) term outcome

• Neurological injuries: brain ultrasound and MRI.

  Short- (during parenteral supplementation, ultrasound) and long- (3 to 5 months of age, MRI) term outcome

• Respiratory immaturity

  Short- (during parenteral supplementation) and long- (3 to 5 months of age) term outcome

Study Arms (2)

Carnitine

EXPERIMENTAL

Intervention 'Parenteral L-carnitine supplementation' Parenteral carnitine supplementation (9 ± 1 mg/kg/d), from day 4, until than enteral nutrition provides sufficient carnitine source.

Drug: Parenteral L-carnitine supplementation

Controle

PLACEBO COMPARATOR

Intervention 'Parenteral supplementation with sterile water'

Drug: Parenteral supplementation with sterile water

Interventions

Parenteral L-carnitine supplementation DRUG

Parenteral carnitine supplementation (9 ± 1 mg/kg/d), from day 4, until than enteral nutrition provides sufficient carnitine source.

Carnitine

Parenteral supplementation with sterile water DRUG

Parenteral supplementation with an equivalent volume of sterile water

Controle

Eligibility Criteria

• Age: Up to 28 Weeks
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17)

You may qualify if:

• Premature newborn admitted in Intensive Care Unit,
• Gestational age minor or equal than 28 weeks and 6 days,
• Needing prolonged parenteral nutrition through a central intravenous catheter,
• Parenteral nutrition started before 6 days of life,
• Both parents (or legal tutor) gave written informed consent for their children,
• Patient affiliated to "Sécurité Sociale" of his parents.

You may not qualify if:

• Severe associated disorder, with a probable short-term death,
• Identified genetic disease,
• Polymalformative syndrome, or severe malformation (heart, brain, others…),
• Inborn error of metabolism,
• Probable transfer of the subject before 25 days of life in another hospital that do not collaborate to this study.

Sponsors & Collaborators

• University Hospital, Tours: lead

Study Sites (2)

UH Porte Madeleine

Orléans, France

Location

Hôpital Clocheville, University Hospital, Tours

Tours, France

Location

Study Officials

• François LABARTHE, MD

  University Hospital, Tours

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 10, 2009
• First Posted: February 11, 2009
• Study Start: July 1, 2008
• Primary Completion: March 1, 2013
• Study Completion: July 1, 2013
• Last Updated: October 22, 2018

Record last verified: 2018-10

Locations

FR (2)