NCT00812019

Brief Summary

The aim of the present dose ranging study is to evaluate the safety, tolerability and immunogenicity of two doses of twelve different formulations of a Cell Culture-Derived H5N1 Subunit Influenza Virus Vaccine, adjuvanted with MF59 or non-adjuvanted, given three weeks apart and followed by a booster dose after 12 months in healthy adults 18 to 40 years of age.

Trial Health

90
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
753

participants targeted

Target at P75+ for phase_1

Timeline
Completed

Started Nov 2008

Typical duration for phase_1

Geographic Reach
2 countries

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2008

Completed
2 months until next milestone

First Submitted

Initial submission to the registry

December 18, 2008

Completed
1 day until next milestone

First Posted

Study publicly available on registry

December 19, 2008

Completed
2 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 1, 2009

Completed
1.7 years until next milestone

Study Completion

Last participant's last visit for all outcomes

November 1, 2010

Completed
Last Updated

August 24, 2020

Status Verified

August 1, 2020

Enrollment Period

4 months

First QC Date

December 18, 2008

Last Update Submit

August 20, 2020

Conditions

Keywords

H5N1 InfluenzaPandemic FluMF59 AdjuvantCell Culture-DerivedSafetyImmunogenicityVaccinationH5N1 influenza pandemic

Outcome Measures

Primary Outcomes (2)

  • immunogenicity, safety and reactogenicity of cell culture-derived H5N1 subunit influenza virus vaccines containing different amounts of antigen and adjuvant.

    52 weeks

  • identification of the optimal adjuvant-antigen dose combination considering antibody titers against the H5N1 strain observed three weeks after two intramuscular doses.

    52 weeks

Secondary Outcomes (1)

  • immunogenicity against heterologous strains of a cell culture-derived H5N1 subunit influenza virus vaccine containing different amounts of antigen and adjuvant.

    52 weeks

Study Arms (12)

3.75_0%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 3.75µg subunit influenza vaccine containing 0% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 3.75_0%MF59

3.75_25%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 3.75µg subunit influenza vaccine containing 25% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 3.75_25%MF59

3.75_50%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 3.75µg subunit influenza vaccine containing 50% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 3.75_50%MF59

3.75_100%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 3.75µg subunit influenza vaccine containing 100% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 3.75_100%MF59

7.5_0%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 7.5µg subunit influenza vaccine containing 0% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 7.5_0%MF59

7.5_25%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 7.5µg subunit influenza vaccine containing 25% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 7.5_25%MF59

7.5_50%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 7.5µg subunit influenza vaccine containing 50% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 7.5_50%MF59

7.5_100%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 7.5µg subunit influenza vaccine containing 100% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 7.5_100%MF59

15_0%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 15µg subunit influenza vaccine containing 0% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 15_0%MF59

15_25%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 15µg subunit influenza vaccine containing 25% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 15_25%MF59

15_50%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 15µg subunit influenza vaccine containing 50% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 15_50%MF59

15_100%MF59

EXPERIMENTAL

Subjects received two 0.5mL vaccinations of cell culture derived H5N1 15µg subunit influenza vaccine containing 100% of MF59 three weeks apart.

Biological: Cell Culture-Derived H5N1 Subunit Influenza Vaccine, 15_100%MF59

Interventions

Two vaccinations of Cell Culture-Derived H5N1 3.75 µg Subunit Influenza Vaccine containing 0% of MF59.

3.75_0%MF59

Two vaccinations of Cell Culture-Derived H5N1 3.75 µg Subunit Influenza Vaccine containing 25% of MF59.

3.75_25%MF59

Two vaccinations of Cell Culture-Derived H5N1 3.75 µg Subunit Influenza Vaccine containing 50% of MF59.

3.75_50%MF59

Two vaccinations of Cell Culture-Derived H5N1 3.75 µg Subunit Influenza Vaccine containing 100% of MF59.

3.75_100%MF59

Two vaccinations of Cell Culture-Derived H5N1 7.5 µg Subunit Influenza Vaccine containing 0% of MF59.

7.5_0%MF59

Two vaccinations of Cell Culture-Derived H5N1 7.5 µg Subunit Influenza Vaccine containing 25% of MF59.

7.5_25%MF59

Two vaccinations of Cell Culture-Derived H5N1 7.5 µg Subunit Influenza Vaccine containing 50% of MF59.

7.5_50%MF59

Two vaccinations of Cell Culture-Derived H5N1 7.5 µg Subunit Influenza Vaccine containing 100% of MF59.

7.5_100%MF59

Two vaccinations of Cell Culture-Derived H5N1 15 µg Subunit Influenza Vaccine containing 0% of MF59.

15_0%MF59

Two vaccinations of Cell Culture-Derived H5N1 15 µg Subunit Influenza Vaccine containing 25% of MF59.

15_25%MF59

Two vaccinations of Cell Culture-Derived H5N1 15 µg Subunit Influenza Vaccine containing 50% of MF59.

15_50%MF59

Two vaccinations of Cell Culture-Derived H5N1 15 µg Subunit Influenza Vaccine containing 100% of MF59.

15_100%MF59

Eligibility Criteria

Age18 Years - 40 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy subjects aged 18-40 years, mentally competent, who have signed an informed consent form after having received a detailed explanation of the study protocol;
  • Able to understand and comply with all study procedures and to complete study diaries, to be contacted, and to be available for study visits.

You may not qualify if:

  • Receipt of another investigational agent within 4 weeks prior to enrollment or before completion of the safety follow-up period in this or in another study; unwilling to refuse participation in another clinical study through the end of this study;
  • Receipt of an H5N1 vaccine;
  • Receipt of another vaccine within 3 weeks prior to Visit 1 or planned vaccination before Visit 5, or within 3 weeks prior to Visit 7 or before Visit 9;
  • Influenza vaccination for the current season within 2 months prior to enrollment (seasonal influenza vaccination is allowed after Visit 5, but no later than 2 months before the booster dose);
  • Experience of any acute disease or infection requiring systemic antibiotic or antiviral therapy (chronic antibiotic therapy for urinary tract prophylaxis is acceptable) within 7 days prior to enrollment and prior to the booster dose or fever within 3 days prior to Visit 1 and Visit 7;
  • Pregnant or breastfeeding, or females of childbearing potential who refuse to use an acceptable method of birth control during the study period for at least 6 weeks following the booster dose; and, if sexually active, who have not used a reliable birth control method for at least two months prior to study entry;
  • Any serious disease, such as: cancer; autoimmune disease; diabetes mellitus type I; diabetes mellitus type II; diabetes relating to genetic defects/syndromes, diseases of the exocrine pancreas or infections; advanced arteriosclerotic disease; moderate or severe chronic obstructive pulmonary disease (COPD); asthma that is greater than mild in severity and/or has exacerbations more than 2 days per week; acute or progressive hepatic disease; acute or progressive renal disease; hyperthyroidism; medically significant bleeding disorders; history of neurological disorders;
  • Body mass index (BMI) ≥ 35 kg/m2 where BMI is for obese and not for high muscle mass;
  • History of (or current) drug or alcohol abuse, surgery planned during the study period or any other condition that in the investigator's opinion would interfere with safety of the subject or the evaluation of study objectives or the visit schedule;
  • Hypersensitivity to any component of the study vaccine;
  • Known or suspected impairment/alteration of immune function;
  • Members of the research staff or their relatives.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Baylor College of Medicine

Houston, Texas, 77030, United States

Location

Universitätsklinikum Gießen und Marburg GmbH

Giessen, 35392, Germany

Location

Related Publications (1)

  • Keitel W, Groth N, Lattanzi M, Praus M, Hilbert AK, Borkowski A, Tsai TF. Dose ranging of adjuvant and antigen in a cell culture H5N1 influenza vaccine: safety and immunogenicity of a phase 1/2 clinical trial. Vaccine. 2010 Jan 8;28(3):840-8. doi: 10.1016/j.vaccine.2009.10.019. Epub 2009 Oct 14.

    PMID: 19835829BACKGROUND

MeSH Terms

Conditions

Influenza, Human

Condition Hierarchy (Ancestors)

Respiratory Tract InfectionsInfectionsOrthomyxoviridae InfectionsRNA Virus InfectionsVirus DiseasesRespiratory Tract Diseases

Study Officials

  • Clinical Scientist

    Seqirus

    STUDY DIRECTOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 18, 2008

First Posted

December 19, 2008

Study Start

November 1, 2008

Primary Completion

March 1, 2009

Study Completion

November 1, 2010

Last Updated

August 24, 2020

Record last verified: 2020-08

Locations