Immune-cell Membrane Trafficking

NCT00638521 | Completed

• 2 other identifiers: 31888-AGM078054-01
• Study Type: observational
• Target: 213
• Locations: 1 country
• Sites: 1

Brief Summary

Organ failure following trauma is a leading cause of morbidity and mortality. It appears that the development of organ failure is a direct result of an altered immune response. This altered response results in the production of circulating factors in the blood that causes direct injury to the injured patients' organs. The mechanism in which this altered immune response occurs is unknown. Based on work we have performed in our laboratory, we believe that this response is initiated on the cell membrane of particular immune cells known as macrophages. Although the cell membrane may appear uniform, it is not. The membrane is composed of specific segments that allow proteins to associate with each other forming receptors that are required for immune cell activation. These specific membrane components are composed of various lipids and cholesterol, and have been termed lipid rafts. Based on our laboratory work it appears that these lipid rafts can be altered following injury. In particular both the lipid and protein content within these raft segments may be altered allowing immune cells to become active leading to the production of factors that directly injure normal cells and organs. Thus, we plan to examine if these laboratory findings can be seen in patients suffering from trauma who develop clinical organ failure at Harborview Medical Center. If this is accomplished, this data will lead to the development of both prognostic and therapeutic interventions for the optimal care of injured patient

Conditions

Severe Trauma

Keywords

lipids; proteins; membrane; lipid raft

Outcome Measures

Primary Outcomes (1)

• Assessment of protein and lipid changes in immune cells following severe injury

  5/08 to 8/12

Secondary Outcomes (1)

• Assessment of severe injury effect on plasma and cellular lipid content

  5/08 to 8/12

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Inclusion criteria:

You may qualify if:

• age 18 years or older, blunt or penetrating trauma and
• one or more of the following: systolic blood pressure less than 90 mmHg at the scene or within one hour of arrival to the Emergency Department, 2) base deficit ≥ -6 within one hour of admission, 3) ISS greater than 25, or 4) more than 6 units of blood transfused in the first 12 hours.-

Sponsors & Collaborators

• University of Washington: lead
• National Institute of General Medical Sciences (NIGMS): collaborator

Study Sites (1)

Harborview Medical Center

Seattle, Washington, 98104, United States

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

whole blood

MeSH Terms

Conditions

Wounds and Injuries

Study Officials

• Joseph Cuschieri, MD

  University of Washington

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Study Principal Investigator

Study Record Dates

• First Submitted: March 12, 2008
• First Posted: March 19, 2008
• Study Start: June 1, 2008
• Primary Completion: August 1, 2012
• Study Completion: June 1, 2013
• Last Updated: December 11, 2013

Record last verified: 2013-12

Locations

US (1)