NCT00629018

Brief Summary

Several studies have documented that transplantation of bone marrow-derived cells (BMC) following acute myocardial infarction is associated with a reduction in infarct scar size and improvements in left ventricular function and perfusion. The available evidence in humans suggests that BMC transplantation is associated with improvements in physiologic and anatomic parameters in both acute myocardial infarction and chronic ischemic heart disease, above and beyond the conventional therapy. In particular, intracoronary application of BMC is proved to be safe and was associated with significant improvement in the left ventricular ejection fraction (LVEF) in patients with chronic heart failure. In contrast to ischemic heart failure, the data on effects of BMC transplantation in patients with dilated cardiomyopathy are limited to pre-clinical studies. In a rat model of dilated cardiomyopathy, intramyocardial delivery of pluripotent mesenchymal cells improved LVEF, possibly through induction of myogenesis and angiogenesis, as well as by inhibition of myocardial fibrosis, suggesting that the beneficial effects of stem cell transplantation in dilated cardiomyopathy may primarily be related to their ability to supply large amounts of angiogenic, antiapoptotic, and mitogenic factors. Similarly, transplantation of cocultured mesenchymal stem cells and skeletal myoblasts was shown to improve LVEF in a murine model of Chagas disease. Study Aim: To define the clinical effects of BMC transplantation in dilated cardiomyopathy in a pilot clinical study investigating the effects of intracoronary CD34+ cell transplantation on functional, structural, neurohormonal, and electrophysiologic parameters in patients with end-stage dilated cardiomyopathy.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
110

participants targeted

Target at P50-P75 for phase_2

Timeline
Completed

Started May 2006

Longer than P75 for phase_2

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

May 1, 2006

Completed
1.8 years until next milestone

First Submitted

Initial submission to the registry

February 25, 2008

Completed
9 days until next milestone

First Posted

Study publicly available on registry

March 5, 2008

Completed
5.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 1, 2013

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

April 1, 2013

Completed
2.1 years until next milestone

Results Posted

Study results publicly available

May 12, 2015

Completed
Last Updated

May 12, 2015

Status Verified

April 1, 2015

Enrollment Period

6.9 years

First QC Date

February 25, 2008

Results QC Date

April 9, 2013

Last Update Submit

April 23, 2015

Conditions

Keywords

Stem CellsHeart FailureDilated Cardiomyopathy

Outcome Measures

Primary Outcomes (2)

  • Heart Failure Mortality

    5 years

  • Changes in Left Ventricular Ejection Fraction

    Left ventricular ejection fraction measured by echocardiography

    5 years

Secondary Outcomes (4)

  • Changes in Exercise Capacity

    5 years

  • Changes in Electrophysiologic Properties of Ventricular Myocardium

    6 months

  • Changes in Plasma Inflammatory Markers

    6 months

  • Changes in Left Ventricular Function

    5 years

Study Arms (2)

SC Group

EXPERIMENTAL

SC therapy,'Bone Marrow Stimulation','CD34+ autologous stem cell transplantation': In the SC group, CD34+ cells were mobilized by granulocyte colony-stimulating factor and collected via apheresis. Patients underwent myocardial scintigraphy and cells were injected in the artery supplying segments with the greatest perfusion defect

Biological: CD34+ autologous stem cell transplantationDrug: Bone Marrow StimulationBiological: SC therapy

Controls

NO INTERVENTION

Patients receiving no cell therapy.

Interventions

Peripheral blood stem cells will be mobilized by daily subcutaneous injections of filgrastim; CD34+ cells will be collected via apheresis and labeled with technetium. Patients will undergo myocardial perfusion scintigraphy for myocardial viability assessment and the collected CD34+ cells will be injected intracoronary in the artery supplying the segments of reduced tracer accumulation

SC Group

Patients will undergo filgrastim stimulation and viability assessment using the same protocol as in Arm 1. However, in this group, no intracoronary stem cell delivery will be performed; the patients will receive placebo (saline).

Also known as: G-CSF stimulation
SC Group
SC therapyBIOLOGICAL

In the SC group, CD34+ cells were mobilized by granulocyte colony-stimulating factor and collected via apheresis. Patients underwent myocardial scintigraphy and cells were injected in the artery supplying segments with the greatest perfusion defect

SC Group

Eligibility Criteria

Age18 Years - 80 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Normal coronary angiogram
  • Left ventricular ejection fraction \< 40%
  • NYHA III or IV heart failure symptoms
  • Bone marrow reactivity (G-CSF test)
  • Presence of viable myocardium

You may not qualify if:

  • Hematologic malignancy
  • Multiorgan failure

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Ljubljana University Medical Center

Ljubljana, 1000, Slovenia

Location

Related Publications (1)

  • Vrtovec B, Poglajen G, Sever M, Lezaic L, Domanovic D, Cernelc P, Haddad F, Torre-Amione G. Effects of intracoronary stem cell transplantation in patients with dilated cardiomyopathy. J Card Fail. 2011 Apr;17(4):272-81. doi: 10.1016/j.cardfail.2010.11.007. Epub 2010 Dec 24.

MeSH Terms

Conditions

Cardiomyopathy, DilatedHeart Failure

Condition Hierarchy (Ancestors)

CardiomegalyHeart DiseasesCardiovascular DiseasesCardiomyopathiesLaminopathiesGenetic Diseases, InbornCongenital, Hereditary, and Neonatal Diseases and Abnormalities

Results Point of Contact

Title
Prof. Dr. Bojan Vrtovec
Organization
UMC Ljubljana

Study Officials

  • Guillermo Torre Amione, MD, PhD

    Methodist DeBakey Heart Center, Houston TX, USA

    STUDY DIRECTOR
  • Francois Haddad, MD

    Stanford University

    STUDY DIRECTOR

Publication Agreements

PI is Sponsor Employee
Yes

Study Design

Study Type
interventional
Phase
phase 2
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
OUTCOMES ASSESSOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
prof. dr. Bojan vrtovec

Study Record Dates

First Submitted

February 25, 2008

First Posted

March 5, 2008

Study Start

May 1, 2006

Primary Completion

April 1, 2013

Study Completion

April 1, 2013

Last Updated

May 12, 2015

Results First Posted

May 12, 2015

Record last verified: 2015-04

Locations