Effects of Dietary Proteins on Hepatic Lipid Metabolism

NCT00558740 | Completed

• 2 other identifiers: protocol 66/07/CE/FBMSNF 310000-109737
• Study Type: observational
• Target: 22
• Locations: 1 country
• Sites: 1

Brief Summary

Individuals submitted to a high-fat or a high-fructose/sucrose diet develop, over a 6 day-period, several features of the metabolic syndrome, including increased plasma triglycerides, increased intrahepatic lipids, and decreased hepatic insulin sensitivity. It has been recently observed that the increase in intrahepatic lipids observed after a high fat diet is largely prevented when protein intake is concomitantly increased. This suggests that dietary protein protects the liver against some of the deleterious effects of a high fat diet. Mechanisms underlying this effect of protein may include an increased hepatic fat oxidation. The aims of this study are:

1. 1.to evaluate the effects of dietary protein on several major pathways involved in hepatic lipid metabolism ( ketogenesis, lipid oxidation, de novo lipogenesis, VLDL-triglyceride secretion
2. 2.to determine whether the decrease in intra-hepatic lipids observed when dietary protein intake is increased are to be attributed to acute or long-term effects of proteins

Conditions

Metabolic Syndrome X

Keywords

Dietary protein; Fructose; VLDL-triglycerides; Hepatic de novo lipogenesis; Lipid oxidation

Outcome Measures

Primary Outcomes (1)

• Whole body lipid oxidation Medium chain triglyceride oxidation Long chain triglyceride oxidation VLDL-triglyceride kinetics Hepatic de novo lipogenesis whole body glucose and glycerol turnover

  acute effects of dietary proteins and after 4 days on a high protein day

Secondary Outcomes (1)

• Energy expenditure Glucagon/insulin ratio Plasma growth hormone concentrations gene expression in subcutaneous adipose tissue Plasma ketone bodies concentrations

  acute effects of dietary proteins and after 4 days on a high protein day

Study Arms (1)

healthy volunteers

Other: high protein intake

Interventions

high protein intake OTHER

acute high protein intake chronic (6-day) high protein intake acute+chronic high protein intake control

healthy volunteers

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Healthy volunteers

You may qualify if:

• age 18-30
• sex male
• BMI 19-25 kg/m2
• sedentary
• good physical health

You may not qualify if:

• family history of diabetes
• use of drugs or illicit substances
• consumption of alcohol \>50 g/week
• vegetarians
• smokers

Sponsors & Collaborators

• University of Lausanne: lead
• Insel Gruppe AG, University Hospital Bern: collaborator

Study Sites (1)

Centre Hospitalier Universitaire Vaudois

Lausanne, Canton of Vaud, CH-1011, Switzerland

Location

MeSH Terms

Conditions

Metabolic Syndrome

Condition Hierarchy (Ancestors)

Insulin Resistance; Hyperinsulinism; Glucose Metabolism Disorders; Metabolic Diseases; Nutritional and Metabolic Diseases

Study Officials

• l Tappy, MD

  University of Lausanne

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: November 14, 2007
• First Posted: November 15, 2007
• Study Start: January 1, 2009
• Primary Completion: April 1, 2009
• Study Completion: April 1, 2009
• Last Updated: February 10, 2012

Record last verified: 2009-11

Locations

CH (1)