NCT00521443

Brief Summary

Various morphological abnormalities of human oocytes were reported to detrimentally affect embryo development. We observed development of frozen thawed embryos derived from oocytes with normal or abnormal morphological features to the blastocyst stage. Cytoplasmic abnormalities of the oocytes were found to negatively affect cryosurvival potential of the embryos.

Trial Health

80
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

August 24, 2007

Completed
3 days until next milestone

First Posted

Study publicly available on registry

August 27, 2007

Completed
Last Updated

August 27, 2007

Status Verified

August 1, 2007

First QC Date

August 24, 2007

Last Update Submit

August 24, 2007

Conditions

Study Arms (12)

Normal

Embryos derived from morphologically normal MII oocytes

Procedure: Cryopreservation, thawing and blastocyst culture

Irregular Shapes

Embryos derived from irregularly shaped oocytes.

Procedure: Cryopreservation, thawing and blastocyst culture

Large PVS

Embryos derived from oocytes with large perivitelline space.

Procedure: Cryopreservation, thawing and blastocyst culture

Dark Zona

Embryos derived from oocytes with dark zona pellucida

Procedure: Cryopreservation, thawing and blastocyst culture

Dark cytoplasm

Embryos derived from oocytes with dark cytoplasm

Procedure: Cryopreservation, thawing and blastocyst culture

Vacuolar cytoplasm

Embryos derived from oocytes with vacuolated cytoplasm

Procedure: Cryopreservation, thawing and blastocyst culture

Central granulation

Embryos derived from oocytes with centrally granulated cytoplasm

Procedure: Cryopreservation, thawing and blastocyst culture

Double extra

Embryos derived from oocytes with double extracytoplasmic abnormalities

Procedure: Cryopreservation, thawing and blastocyst culture

Double combined

Embryos derived from oocytes with any combination of one extracytoplasmic and one cytoplasmic anomaly

Procedure: Cryopreservation, thawing and blastocyst culture

Double cytoplasmic

Embryos derived from oocytes with any two cytoplasmic anomalies

Procedure: Cryopreservation, thawing and blastocyst culture

Triple extra

Embryos derived from oocytes with triple extracytoplasmic anomaly

Procedure: Cryopreservation, thawing and blastocyst culture

Triple combined

Embryos derived from oocytes with triple combined anomalies

Procedure: Cryopreservation, thawing and blastocyst culture

Interventions

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Central granulationDark ZonaDark cytoplasmDouble combinedDouble cytoplasmicDouble extraIrregular ShapesLarge PVSNormalTriple combinedTriple extraVacuolar cytoplasm

Eligibility Criteria

Age18 Years - 45 Years
Sexfemale
Age GroupsAdult (18-64)

You may qualify if:

  • Human embryos derived from morphologically normal or abnormal mature oocytes that fertilized normally by microinjection procedure.
  • Embryos were obtained from couples who refused to freeze their supernumerary embryos after fresh embryo transfer in an assisted reproduction treatment cycle.

You may not qualify if:

  • Embryos obtained after microinjection to immature oocytes taht were in vitro matured in culture.
  • Embryos obtained from abnormally fertilized zygotes.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

VKV American Hospital

Istanbul, 34365, Turkey (Türkiye)

Location

Related Publications (1)

  • Balaban B, Ata B, Isiklar A, Yakin K, Urman B. Severe cytoplasmic abnormalities of the oocyte decrease cryosurvival and subsequent embryonic development of cryopreserved embryos. Hum Reprod. 2008 Aug;23(8):1778-85. doi: 10.1093/humrep/den127. Epub 2008 May 12.

MeSH Terms

Interventions

Cryopreservation

Intervention Hierarchy (Ancestors)

Tissue PreservationHistocytological Preparation TechniquesCytological TechniquesClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisHistological TechniquesPreservation, BiologicalTherapeuticsInvestigative Techniques

Study Officials

  • Basak Balaban, B.Sc.

    Assisted Reproduction Unit of the VKV American Hospital of Istanbul

    STUDY DIRECTOR

Study Design

Study Type
observational
Observational Model
DEFINED POPULATION
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER

Study Record Dates

First Submitted

August 24, 2007

First Posted

August 27, 2007

Last Updated

August 27, 2007

Record last verified: 2007-08

Locations