Procurement of Tissue Samples for Cell Cultures and Analyses

NCT00435877 | Terminated

• 2 other identifiers: 99990704407-D-N044
• Study Type: observational
• Target: 2
• Locations: 1 country
• Sites: 1

Brief Summary

This study will determine if bone marrow stromal cells can be used as a graft for regeneration of bony defects. These cells have the ability to form new bone and to support the formation of blood. Stromal cells have been identified in other tissues, such as the dermis (underside of skin), spleen, thymus and fat, but it is not known whether these cells can make bone and support blood formation as well as bone marrow stromal cells. Patients undergoing surgery at Suburban Hospital in Bethesda, Maryland, in which some of the bone, dermis, spleen, thymus or fat tissue is removed may be eligible for this study. For this study, a small piece of the tissue that is removed during surgery for examination by a pathologist will be used for research. Stromal cells from the specimen will be collected and grown in the laboratory. The genes and proteins in the different cell types will be compared, and the ability of stromal cells from the different tissues to make bone and to support the formation of blood will be studied.

Conditions

Bone Regeneration; Fracture Healing; Cell Culture Techniques; Tissue Engineering; Differentiation

Keywords

Connective Tissue; Stromal Cells; Surgical Waste; In Vivo Studies; Tissue Analysis

Eligibility Criteria

• Age: 18 Years - 80 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Any patient over the age of 18 years who is undergoing a clinically indicated or elective surgery that entails removal of bone with red marrow,periosteum, dermis, spleen, thymus, muscle and adipose tissue.
• All patients must sign a document of informed consent indicating their understanding that the specimens collected and demographic information provided is only for research purposes.

You may not qualify if:

• Any patient that has a history of metabolic bone disease.
• Any patient that has cancer of any type, or has been treated for cancer of any type.
• Any patient that has been treated with a reagent known to affect bone metabolism (bisphosphonates, steroids, vitamin D)

Sponsors & Collaborators

• National Institute of Dental and Craniofacial Research (NIDCR): lead

Study Sites (1)

Suburban Hospital

Bethesda, Maryland, 20814, United States

Location

Related Publications (3)

• Bianco P, Riminucci M, Kuznetsov S, Robey PG. Multipotential cells in the bone marrow stroma: regulation in the context of organ physiology. Crit Rev Eukaryot Gene Expr. 1999;9(2):159-73. doi: 10.1615/critreveukargeneexpr.v9.i2.30.

  PMID: 10445154 BACKGROUND

• Friedenstein AJ, Chailakhyan RK, Latsinik NV, Panasyuk AF, Keiliss-Borok IV. Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues. Cloning in vitro and retransplantation in vivo. Transplantation. 1974 Apr;17(4):331-40. doi: 10.1097/00007890-197404000-00001. No abstract available.

  PMID: 4150881 BACKGROUND

• Gronthos S, Franklin DM, Leddy HA, Robey PG, Storms RW, Gimble JM. Surface protein characterization of human adipose tissue-derived stromal cells. J Cell Physiol. 2001 Oct;189(1):54-63. doi: 10.1002/jcp.1138.

  PMID: 11573204 BACKGROUND

Study Officials

• Pamela G Robey, Ph.D.

  National Institute of Dental and Craniofacial Research (NIDCR)

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Time Perspective: PROSPECTIVE
• Sponsor Type: NIH

Study Record Dates

• First Submitted: February 15, 2007
• First Posted: February 16, 2007
• Study Start: January 29, 2007
• Study Completion: November 5, 2012
• Last Updated: December 17, 2019

Record last verified: 2012-11-05

Locations

US (1)