Vaccination With Autologous Breast Cancer Cells Engineered to Secrete Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) in Metastatic Breast Cancer Patients

NCT00317603 | Completed Phase 1 Results

• 1 other identifier: 05-111
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 2

Brief Summary

The purpose of this trial is to test the safety of a vaccine made from a patient's own breast cancer cells, and determine if this vaccine will delay or stop the growth of the cancer. The vaccine is made by genetically modifying a patient's own tumor cells to secrete granulocyte-macrophage colony-stimulating factor (GM-CSF) to activate the immune response.

Conditions

Breast Cancer

Keywords

autologous vaccination; GM-CSF; adenoviral mediated gene transfer; Stage IV breast cancer

Outcome Measures

Primary Outcomes (1)

• Minimum Number of Vaccine Doses Created Using Participant Tumor Sample

  Tumor samples were obtained via malignant effusion or a surgically accessible tumor nodule of 2 cm in greatest diameter. Tumor cells were processed to single cell suspension and transduced with adenoviral vector encoding human Granulocyte-macrophage colony-stimulating factor (GM-CSF). Then, the cells washed extensively and irradiated with 10,000 cGy. Over the next 14 days, sterility cultures were tested for endotoxin and mycoplasma contamination. Individual vaccine cell dose and number varied depending on the final cell yield from vaccine production. The minimal dose was 1 x 10\^5 cells and the maximal dose was 1 x 10\^7 cells.

  Vaccine doses created and banked soon after registration, up to 8 days.

Secondary Outcomes (2)

• Number of Participants With Grade 3 or Higher Adverse Events

  Up to 58 Months

• Number of Participants With RECIST Criteria Responses

  Up to 14 Years

Study Arms (1)

Vaccine

EXPERIMENTAL

Vaccinations will be administered on days 1,8,15 and every two weeks thereafter until the supply of vaccine has been exhausted or the patient is removed from study. As indicated in 5.2.5, vaccine cell dosage will be approximately 1x10 7 , 4x10 6 , 1x10 6 , or 1x10 5 depending on the final cell yield.

Biological: Autologous, Lethally Irradiated Breast Cancer Cells

Interventions

Autologous, Lethally Irradiated Breast Cancer Cells BIOLOGICAL

Vaccine will be administered on days 1, 8, 15, 29 and then every 2 weeks until the supply of vaccine runs out

Vaccine

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Histologically confirmed Stage IV breast cancer
• Prior banked malignant effusion or significant malignant effusion for tumor harvest or surgically-accessible tumor nodule of at least 2cm in greatest diameter by physical exam, magnetic resonance imaging (MRI) or computed tomography (CT) scan
• Must have received at least one prior regimen of chemotherapy for metastatic disease
• Patients with HER2 positive tumors must have received at least one prior trastuzumab-based therapy in the metastatic setting, and may not receive trastuzumab therapy and vaccine treatment concurrently
• Patients may receive concurrent bisphosphonate therapy and/or erythropoetin therapy at any point while on study
• ECOG performance status 0 or 1
• Estimated life expectancy of greater than or equal to 6 months
• years of age or older
• Greater than 4 weeks from immunotherapy, or systemic glucocorticoid therapy
• Adequate recovery from drug-related toxicities from prior systemic therapies
• Adequate recovery from recent surgery and radiation therapy
• Greater than 6 months since bone marrow or peripheral blood stem cell transplant

You may not qualify if:

• Urgent need for cytotoxic chemotherapy, radiotherapy, or surgery in the next 60 days
• Uncontrolled active infection or illness
• Psychiatric illness/social situation that would limit study compliance
• Pregnant or nursing mothers
• Evidence of HIV infection
• Previous participation in an adenovirus-based trial
• Concurrent invasive malignancy

Sponsors & Collaborators

• Dana-Farber Cancer Institute: lead
• Brigham and Women's Hospital: collaborator

Study Sites (2)

Brigham and Women's Hospital

Boston, Massachusetts, 02115, United States

Location

Dana-Farber Cancer Institute

Boston, Massachusetts, 02115, United States

Location

Related Publications (1)

• Anderson KS, Erick TK, Chen M, Daley H, Campbell M, Colson Y, Mihm M, Zakka LR, Hopper M, Barry W, Winer EP, Dranoff G, Overmoyer B. The feasibility of using an autologous GM-CSF-secreting breast cancer vaccine to induce immunity in patients with stage II-III and metastatic breast cancers. Breast Cancer Res Treat. 2022 Jul;194(1):65-78. doi: 10.1007/s10549-022-06562-y. Epub 2022 Apr 28.

  PMID: 35482127 DERIVED

MeSH Terms

Conditions

Breast Neoplasms

Condition Hierarchy (Ancestors)

Neoplasms by Site; Neoplasms; Breast Diseases; Skin Diseases; Skin and Connective Tissue Diseases

Results Point of Contact

• Title: Beth Overmoyer
• Organization: Mass General Brigham

Beth_Overmoyer@DFCI.HARVARD.EDU

617 632 3800

Study Officials

• Beth Overmoyer, MD

  Dana-Farber Cancer Institute

  PRINCIPAL INVESTIGATOR

Publication Agreements

• PI is Sponsor Employee: Yes

Study Design

• Study Type: interventional
• Phase: phase 1
• Allocation: NA
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: April 21, 2006
• First Posted: April 25, 2006
• Study Start: January 1, 2006
• Primary Completion: May 1, 2008
• Study Completion: June 1, 2021
• Last Updated: April 1, 2022
• Results First Posted: October 14, 2021

Record last verified: 2022-03

Locations

US (2)