Analysis of Expression of Nitric Oxide Syntheses and Their Functional Role in Human Urothelium

NCT00253006 | Terminated

• 1 other identifier: UA_H_Ka-05104-m
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

Painful bladder syndrome (PBS)/interstitial cystitis (IC) may be due to the actions of nitric oxide (NO) in the bladder tissue. NO is a gaseous substance with a very short half-life, synthesized by a group of NO-synthase-enzymes in many tissues. The goal of this study is to illuminate bladder tissue production of NO in individuals with PBS and healthy individuals, by quantification of NO and NO-enzyme expression by different molecular biological methods.

Conditions

Interstitial Cystitis; Bladder Diseases; Urinary Incontinence

Keywords

NO; mRNA-expression; pathophysiology; painful bladder syndrome

Interventions

Tissue examination for protein- and mRNA-expression of NO PROCEDURE

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• PBS/IC according to International Continence Society (ICS)
• Cystoscopy with bladder distension and deep biopsies within last 5 years
• Cystometry within last 5 years
• Normal urine culture within last month
• Benign non-invasive bladder disease
• No PBS/IC
• Normal urine culture
• No functional bladder disease of known nature (ex. infravesical obstruction)

You may not qualify if:

• Age under 18 years
• Inability to understand instructions
• Pregnancy and lactation

Sponsors & Collaborators

• Copenhagen University Hospital at Herlev: lead
• Zealand University Hospital: collaborator

Study Sites (1)

Copenhagen University Hospital at Herlev

Herlev, DK- 2730, Denmark

Location

Related Publications (3)

• Birder LA, Wolf-Johnston A, Buffington CA, Roppolo JR, de Groat WC, Kanai AJ. Altered inducible nitric oxide synthase expression and nitric oxide production in the bladder of cats with feline interstitial cystitis. J Urol. 2005 Feb;173(2):625-9. doi: 10.1097/01.ju.0000145900.22849.1d.

  PMID: 15643277 BACKGROUND

• Bouchelouche K, Nordling J. Recent developments in the management of interstitial cystitis. Curr Opin Urol. 2003 Jul;13(4):309-13. doi: 10.1097/00042307-200307000-00007.

  PMID: 12811295 BACKGROUND

• Logadottir YR, Ehren I, Fall M, Wiklund NP, Peeker R, Hanno PM. Intravesical nitric oxide production discriminates between classic and nonulcer interstitial cystitis. J Urol. 2004 Mar;171(3):1148-50; discussion 50-1. doi: 10.1097/01.ju.0000110501.96416.40.

  PMID: 14767289 BACKGROUND

MeSH Terms

Conditions

Cystitis, Interstitial; Urinary Bladder Diseases; Urinary Incontinence

Interventions

Proteins

Condition Hierarchy (Ancestors)

Cystitis; Urologic Diseases; Female Urogenital Diseases; Female Urogenital Diseases and Pregnancy Complications; Urogenital Diseases; Male Urogenital Diseases; Urination Disorders; Lower Urinary Tract Symptoms; Urological Manifestations; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Amino Acids, Peptides, and Proteins

Study Officials

• Pierre Bouchelouche, MD

  Roskilde County University Hospital at Koege, Dept. of Clinical Biochemistry

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: DEFINED POPULATION
• Time Perspective: OTHER
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: November 11, 2005
• First Posted: November 15, 2005
• Study Start: January 1, 2006
• Study Completion: January 1, 2008
• Last Updated: October 9, 2006

Record last verified: 2006-10

Locations

DK (1)