NCT00240526

Brief Summary

To evaluate the persistence of anti-HBs antibodies up to 16, 17, 18, 19 and 20 years after administration of the first dose of the study vaccine. The Protocol Posting has been updated in order to comply with the FDA Amendment Act, Sep 2007. No additional subjects will be recruited during this long-term follow-up study and no vaccine will be administered.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
79

participants targeted

Target at P25-P50 for phase_4

Timeline
Completed

Started Oct 2003

Longer than P75 for phase_4

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

October 1, 2003

Completed
2 years until next milestone

First Submitted

Initial submission to the registry

October 13, 2005

Completed
5 days until next milestone

First Posted

Study publicly available on registry

October 18, 2005

Completed
4.4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 1, 2010

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

March 1, 2010

Completed
5 months until next milestone

Results Posted

Study results publicly available

July 15, 2010

Completed
Last Updated

December 7, 2016

Status Verified

October 1, 2016

Enrollment Period

6.4 years

First QC Date

October 13, 2005

Results QC Date

June 14, 2010

Last Update Submit

October 14, 2016

Conditions

Keywords

Hepatitis B immunoglobulinHepatitis B antibody persistence

Outcome Measures

Primary Outcomes (6)

  • Anti-hepatitis B Surface Antigen (Anti-HBs) Antibody Concentration as Measured by Enzyme-Linked Immunosorbent Assay (ELISA).

    Concentrations given as GMC expressed as milli-international unit per millilitre (mIU/mL). Note: At Year 15 and 16, a commercial ELISA was used. From Year 17 to Year 20, anti-HBs antibody concentrations were tested with a validated in-house assay with cut-off 3.3mIU/mL.

    At Years 15, 16, 17, 18, 19 and 20

  • Anti-hepatitis B Surface Antigen (Anti-HBs) Antibody Concentration as Measured by ChemiLuminescence ImmunoAssay (CLIA).

    Concentrations given as GMC expressed as milli-international unit per millilitre (mIU/mL). Note: There was a change of assay kit at Year 19 time-point, thus for the sake of bridging, blood samples corresponding to Year 19 were re-tested with new CLIA. At Year 19 and 20, anti-HBs antibody concentrations tested with the CLIA with cut-off 6.2 mIU/mL.

    At Years 19 and 20

  • Number of Subjects With Anti-hepatitis B Surface Antigen (Anti-HBs) Antibody Concentrations Above Pre-defined Cut-off Values Enzyme-Linked Immunosorbent Assay (ELISA).

    Anti-hepatitis B surface antigen (anti-HBs) antibody cut-off values assessed include 1.0 and 10 mIU/mL.

    At Years 15, 16, 17, 18, 19 and 20

  • Adjusted Number of Subjects With Anti-hepatitis B Surface Antigen (Anti-HBs) Antibody Concentrations Above Pre-defined Cut-off Values as Measured by ChemiLuminescence ImmunoAssay (CLIA)

    Anti-hepatitis B surface antigen (anti-HBs) antibody cut-off values assessed include 1.0 and 10 mIU/mL. Note: Missing CLIA anti-HBs concentrations, for subjects with ELISA results available, are estimated by multiple imputations and GMCs and number of subjects were adjusted for these imputations.

    At Years 19 and 20

  • Number of Subjects With Positive Results for Serological Markers for Hepatitis B Infection

    Serological markers for hepatitis B infection assessed are hepatitis B surface antigen (HBsAg), antibodies to hepatitis B core antigen (anti-HBc), hepatitis B e antigen (HBeAg) and antibodies to hepatitis B e antigen (anti-HBe).

    At Years 15, 16, 17, 18, 19 and 20

  • Number of Subjects With Different Hepatitis B Infection Statuses

    Categories hepatitis B (HB) infection: 1. Chronic infection: HBsAg and anti-HBc pos (pos) in more than two consecutive samples 2. False positive: single HB marker (HBsAg, HBeAg, anti-HBc) pos + all other markers negative (neg) in one sample. Consecutive time points all neg. 3. Possible subclinical breakthrough infection: One or more HB markers pos in one or more consecutive samples. 4. Isolated natural booster: \>4-fold increase of anti-HBs concentrations if \<100 mIU/mL at previous sample OR \>2- fold increase of anti-HBs concentrations if \>=100 mIU/mL at previous sample + other markers neg

    Over the entire follow up period (Final assessment of clinical significance was analyzed after the Year 20 time point)

Study Arms (4)

Engerix 4D + HBIg Group

EXPERIMENTAL

Subjects received Engerix™ (hepatitis B vaccine \[HBV\]) at Month 0, 1, 6 and 60 with hepatitis B immunoglobulins (HBIg) administered concomitantly at birth in the opposite arm.

Biological: Engerix™ -BBiological: Hepatitis B immunoglobulin (HBIg)

Engerix 3D + HBIg Group

EXPERIMENTAL

Subjects received Engerix™ (hepatitis B vaccine \[HBV\]) at Month 0, 1, and 6 with hepatitis B immunoglobulins (HBIg) administered concomitantly at birth in the opposite arm.

Biological: Engerix™ -BBiological: Hepatitis B immunoglobulin (HBIg)

Engerix 4D

EXPERIMENTAL

Subjects received Engerix™ (hepatitis B vaccine \[HBV\]) at Month 0, 1, 6 and 60.

Biological: Engerix™ -B

Engerix 3D Group

EXPERIMENTAL

Subjects received Engerix™ (hepatitis B vaccine \[HBV\]) at Month 0, 1, and 6.

Biological: Engerix™ -B

Interventions

Engerix™ -BBIOLOGICAL

3 (Groups A and C) or 4 (Groups B and D) intramuscular injections during the primary study

Engerix 3D + HBIg GroupEngerix 3D GroupEngerix 4DEngerix 4D + HBIg Group

1 intramuscular injections at birth (primary study)

Engerix 3D + HBIg GroupEngerix 4D + HBIg Group

Eligibility Criteria

Age16 Years - 20 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64)

You may qualify if:

  • Subjects who had received at least one dose of the study vaccine in the primary study
  • Written informed consent obtained from each subject before each blood sampling visit

You may not qualify if:

  • None

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

GSK Investigational Site

Bangkok, 10330, Thailand

Location

Related Publications (1)

  • Poovorawan Y, Chongsrisawat V, Theamboonlers A, Leroux-Roels G, Crasta PD, Hardt K. Persistence and immune memory to hepatitis B vaccine 20 years after primary vaccination of Thai infants, born to HBsAg and HBeAg positive mothers. Hum Vaccin Immunother. 2012 Jul;8(7):896-904. doi: 10.4161/hv.19989. Epub 2012 Jul 1.

Related Links

MeSH Terms

Conditions

Hepatitis B

Interventions

hepatitis B hyperimmune globulin

Condition Hierarchy (Ancestors)

Blood-Borne InfectionsCommunicable DiseasesInfectionsHepadnaviridae InfectionsDNA Virus InfectionsVirus DiseasesHepatitis, Viral, HumanHepatitisLiver DiseasesDigestive System Diseases

Limitations and Caveats

A decrease in the specificity of the anti-HB ELISA assay had been observed in some studies for low levels of antibody (10-100 mIU/mL). Additional tables have been generated following partial retesting and reanalysis of the results at Years 19 and 20.

Results Point of Contact

Title
GSK Response Center
Organization
GlaxoSmithKline

Study Officials

  • GSK Clinical Trials

    GlaxoSmithKline

    STUDY DIRECTOR

Publication Agreements

PI is Sponsor Employee
No
Restriction Type
OTHER
Restrictive Agreement
Yes

Study Design

Study Type
interventional
Phase
phase 4
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

October 13, 2005

First Posted

October 18, 2005

Study Start

October 1, 2003

Primary Completion

March 1, 2010

Study Completion

March 1, 2010

Last Updated

December 7, 2016

Results First Posted

July 15, 2010

Record last verified: 2016-10

Data Sharing

IPD Sharing
Will share

Patient-level data for this study will be made available through www.clinicalstudydatarequest.com following the timelines and process described on this site.

Available IPD Datasets

Clinical Study Report (100450)Access
Statistical Analysis Plan (100450)Access
Study Protocol (100450)Access
Dataset Specification (100450)Access
Individual Participant Data Set (100450)Access
Informed Consent Form (100450)Access

Locations