NCT00213707

Brief Summary

Re-infection of the liver graft occurs universally following liver transplantation for HCV-induced end stage liver disease. Interestingly, the manifestation of HCV infection ranging from an asymptomatic carrier state to rapid progression to liver cirrhosis is extremely variable from one patient to another. The host and viral factors being responsible for the different course of HCV infection are poorly understood. Our study will focus on dendritic cells which are known to play a critical role in the control of viral infection. Viral factors (quasispecies evolution) will also be analysed and Th1 Th2 cytokines produced in the serum evaluated.Thirty liver transplanted patients will be included : 20 patients transplanted for HCV-related disease and 10 controls transplanted for non HCV-related disease. Blood samples will be taken just before transplantation and at different time -points up to 5 years after transplantation. Function of blood monocyte-derived DC will be evaluated. Circulating blood plasmacytoid (pDC) and myeloid DC (mDC) sub-populations will be enumerated. HCV quasispecies evolution will be analysed. This study may help to define prognostic markers on progression of hepatitis C in liver transplantation and allow the development of new immunotherapeutic drugs

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
30

participants targeted

Target at below P25 for all trials

Timeline
Completed

Started May 2003

Longer than P75 for all trials

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

May 1, 2003

Completed
2.4 years until next milestone

First Submitted

Initial submission to the registry

September 13, 2005

Completed
8 days until next milestone

First Posted

Study publicly available on registry

September 21, 2005

Completed
4.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 1, 2010

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2011

Completed
Last Updated

February 25, 2009

Status Verified

February 1, 2009

Enrollment Period

7 years

First QC Date

September 13, 2005

Last Update Submit

February 24, 2009

Conditions

Keywords

Dendritic cellsliver transplantationhepatitis C virusquasispeciesvirological markersbiochemical markersimmunological markersclinical markers

Outcome Measures

Primary Outcomes (1)

  • Function of blood circulating DC at day 7 and 1, 3, 6, 9, 12 months, 2 and 5 years after liver transplantation depending on the clinical outcome (mild or severe hepatitis, cirrhosis).

    5 years

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients for liver transplantation indicated after chronic hepatitis C.

You may qualify if:

  • Liver transplanted HCV infected patients :
  • Adults, HIV(-),HBV(-)
  • liver transplanted for an end stage liver disease associated to HCV.
  • Genotype determined
  • written agreement to participate to the study
  • Control patients :
  • Adults, HIV(-), HBV(-), HCV(-)
  • liver transplanted for a non HCV liver disease
  • written agreement to participate to the study.

You may not qualify if:

  • HIV(+), HBV(+)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Francoise Stoll-Keller

Strasbourg, France

Location

Biospecimen

Retention: SAMPLES WITH DNA

Serum peripheral blood mononuclear cells

MeSH Terms

Conditions

Hepatitis C

Condition Hierarchy (Ancestors)

Blood-Borne InfectionsCommunicable DiseasesInfectionsHepatitis, Viral, HumanVirus DiseasesFlaviviridae InfectionsRNA Virus InfectionsHepatitisLiver DiseasesDigestive System Diseases

Study Officials

  • Francoise Stoll-Keller, MD

    Hopitaux Universitaires de Strasbourg

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER

Study Record Dates

First Submitted

September 13, 2005

First Posted

September 21, 2005

Study Start

May 1, 2003

Primary Completion

May 1, 2010

Study Completion

May 1, 2011

Last Updated

February 25, 2009

Record last verified: 2009-02

Locations