Biological Specimen Collection for Laboratory Methods Development and Training Purposes

NCT00025831 | Completed

• 2 other identifiers: 02001702-C-0017
• Study Type: observational
• Target: 200
• Locations: 1 country
• Sites: 1

Brief Summary

This study will collect biological samples-buccal cells, blood sample, skin sample-to be used in developing and testing laboratory methods for measuring and analyzing genes. Such methods can be used for research on identifying genetic factors that may affect a person's cancer risk. All individuals age 21 and older may participate in this study. Participants will provide one or more of the following samples:

• Buccal (mouth) cells - obtained by swishing a small amount of mouthwash in the mouth or by swabbing or bushing the inside of the cheek with a swab or brush.
• Blood - obtained by pricking the finger and collecting the drops or by blood drawing through a needle placed in an arm vein.
• Skin - obtained by a punch biopsy on the inner upper arm. For this procedure, the skin is anesthetized and a small piece of skin is removed with a sharp instrument similar to a cookie cutter. The wound is then covered and held together with a sterile bandage. A small scar, approximately 1/8-inch long, will be left. Most participants will be asked to provide only a buccal sample; blood and/or skin samples will be requested from a few participants. The blood and skin samples may be used to grow cell lines; that is, to make them grow indefinitely for research uses.

Conditions

Healthy

Keywords

Buccal; DNA; Pooling; Genotyping; Mouthwash; Healthy Volunteer; HV; Normal Control

Eligibility Criteria

• Age: 21 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• All individuals age 21 and older will be eligible.

You may not qualify if:

• Participants who have volunteered for previous DCEG studies within the past three years in which buccal or blood samples were collected will be excluded because the samples collected in the current study may be combined with some previously collected samples and duplication of subjects is to be avoided.
• Individuals with known active oral disease will be excluded from the buccal cell collection component, and those known to form keloids will be excluded from the skin biopsy component.

Sponsors & Collaborators

• National Cancer Institute (NCI): lead

Study Sites (1)

National Cancer Institute (NCI)

Bethesda, Maryland, 20892, United States

Location

Related Publications (1)

• Buetow KH, Edmonson M, MacDonald R, Clifford R, Yip P, Kelley J, Little DP, Strausberg R, Koester H, Cantor CR, Braun A. High-throughput development and characterization of a genomewide collection of gene-based single nucleotide polymorphism markers by chip-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):581-4. doi: 10.1073/pnas.98.2.581. Epub 2001 Jan 2.

  PMID: 11136232 BACKGROUND

Related Links

• NIH Clinical Center Detailed Web Page

Study Design

• Study Type: observational
• Sponsor Type: NIH

Study Record Dates

• First Submitted: October 26, 2001
• First Posted: October 29, 2001
• Study Start: October 1, 2001
• Study Completion: September 1, 2005
• Last Updated: March 4, 2008

Record last verified: 2005-09

Locations

US (1)